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ID   PBR329     preliminary; circular DNA; SYN; 4150 BP.
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AC   J01753; L08859; VB0005; ATCC37264;
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DT   13-JUN-1985 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBR329 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4150; 160-340; 3440-3560
RC   pBR329 from pBR327 & pBR328
RA   Covarrubias L., Bolivar F.;
RT   "Construction and characterization of new cloning vehicles: VI.
RT   plasmid pBR329, a new derivative of pBR328 lacking the
RT   482-base-pair inverted duplication";
RL   Gene 17:79-89(1982).
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RN   [2]
RP   1-5996
RC   pBR329
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
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CC   Revised 02-APR-1986 by F. Pfeiffer
CC   Reference [1] only sequenced bases 160-340 and 3440-3560.
CC   The other regions are from previously published sequences for
CC   pBR327 and Tn981, as listed in figure 5 of [1].
CC   This plasmid does not carry the inverted duplication region found in
CC   pBR328.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBR329)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR328)(Tn981)(pBR325)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR327 EcoRI 3273bp 3272..3272
FT                   S1 nuclease7791634
FT                   2. pBR328 HhaI-HhaI 1364bp 3170..4534, cat gene
FT                   BAL31 nuclease:BAL31 nuclease, 877bp
FT                   -> pBR329 4150bp"
FT   CDS             complement(3708..4151)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   CDS             complement(1..216)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             317..1507
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             complement(2440..3228)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique AatI"
FT   misc_binding    0..0
FT                   /note="SIT unique AsuII"
FT   misc_binding    0..0
FT                   /note="SIT unique AvaI"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique HgiEII"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique Tth111I"
FT   misc_binding    0..0
FT                   /note="SIT unique BalI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NcoI"
FT   misc_binding    0..0
FT                   /note="SIT unique NruI"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique SphI"
FT   misc_binding    0..0
FT                   /note="SIT unique XmaIII"
FT   misc_binding    0..0
FT                   /note="SIT unique XmnI"
FT   misc_feature    complement(1..237)
FT                   /note="from pBR325"
FT   misc_feature    237..1662
FT                   /note="from pBR325"
FT   misc_feature    complement(1066..1542)
FT                   /note="from pBR325"
FT   misc_feature    1660..3498
FT                   /note="from pBR325"
FT   misc_feature    complement(3499..4151)
FT                   /note="from pBR325"
XX
SQ   Sequence 4150 BP; 970 A; 1121 C; 1021 G; 1038 T; 0 other;
     ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgatgt
     ttgacagctt atcatcgata agctttaatg cggtagttta tcacagttaa attgctaacg
     cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
     cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
     cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
     gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
     cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
     cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     catgagcgct tgtttcggcg tgggtatggt ggcaggcccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc
     acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcc
     acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
     atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
     cgtccgccat ctccagcagc cgcacgcggc gcatctcggg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattt aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtctagg catttgagaa gcacacggtc acactgcttc cggtagtcaa
     taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac
     gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
     gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     ccatacggaa
//