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ID   PBRH4      preliminary; circular DNA; SYN; 4363 BP.
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AC   M10366; ATCC37071;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBRH4 - complete, promoter.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-71
RC   pIM4 from pBR322 & CaMV genome
RC   pBRH4 from pBR322 & linker
RC   pMM40, pMM10, pMM116, pMM140, pMM128 from pBRH4 & CaMV
RA   McKnight T.D., Meagher R.B.;
RT   "Isolation and mapping of small cauliflower mosaic virus DNA
RT   fragments active as promoters in Escherichia coli";
RL   J. Virol. 37:673-682(1981).
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RN   [2]
RC   pBRH4 from pBR322 & linker
RA   Heyneker H.L.;
RT   ;
RL   Unpublished (1981).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   This is a promoter cloning plasmid vector using expression of
CC   tetracycline resistance for selection. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 208965
CC   NM (pBRH4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-HindIII 31bp
FT                   \ 4360..4361..30, tet promoter/4330bp
FT                   :S1 nuclease
FT                   :EcoRI linker 8bp tgaattca
FT                   -> pBRH4 4000bp"
FT   -               1..29
FT                   /note="pBR322 1..29 29bp
FT                   HindIII = A^AGCTT
FT                   \           tg"
FT   -               30..31
FT                   /note="tg 2bp
FT                   \      tg
FT                   EcoRI = G^AATTC"
FT   -               32..4363
FT                   /note="pBR322 30..4361 4332bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet);
FT                   reporter gene"
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SQ   Sequence 4363 BP; 983 A; 1210 C; 1135 G; 1035 T; 0 other;
     ttctcatgtt tgacagctta tcatcgatat gagctttaat gcggtagttt atcacagtta
     aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
     ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
     ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta
     tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc
     cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg
     accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg
     atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
     gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
     tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
     catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga
     ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg
     attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
     cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa
     gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
     acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg
     ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
     ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
     gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
     cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg
     caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt
     gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac
     aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc
     gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga
     ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
     ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
     acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
     acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa
//