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ID   PBRINT     preliminary; circular DNA; SYN; 6015 BP.
XX
AC   L25311;
XX
DT   20-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBRINT - complete.
XX
KW   cloning vector; ampicillin resistance; lacZ gene;
KW   beta-d-galactoside galactohydrolase; bla gene; cat gene;
KW   beta-galactosidase; beta-lactamase; beta-lactamase transporter;
KW   chloramphenicol acetyltransferase; chloramphenicol resistance;
KW   penicillin amido-beta-lactam hydrolase.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6015
RC   plasmid from pBR322 & lacZ gene
RC   pBRINT from plasmid & pCAT19
RA   Balbas P., Alvarado X., Bolivar F., Valle F.;
RT   "pBRINT: Vector for chromosomal insertion of cloned DNA";
RL   Gene 136:211-213(1993).
XX
CC   NCBI gi: 408948
CC   NM (pBRINT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. E. coli lacZ gene 2966bp, AA8 to end
FT                   -> plasmid
FT                   1. plasmid EcoRV, lacZ gene 1117bp + 1849bp
FT                   2. pCAT19 957bp, cat gene
FT                   \ pBR325 BstBI/AsuII-BstBI/AsuII 952bp
FT                   \ 4356..5308, Tn9 cat gene
FT                   -> pBRINT 6015bp"
FT   misc_binding    1114..1133
FT                   /note="MCS"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lac);
FT                   partial"
XX
SQ   Sequence 6015 BP; 1457 A; 1545 C; 1576 G; 1437 T; 0 other;
     cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
     gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
     tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt
     acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
     aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
     tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
     ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
     gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
     attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
     gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
     ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
     gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
     cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
     ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
     tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
     ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
     gatgagcaga cgatggtgca ggattgcagg tccggtaccc ggggatcctc tagagtccga
     ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
     taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg
     ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gcgacctgca
     atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat
     ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt
     gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg
     atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc
     tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc
     aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc
     accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg
     ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt
     tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg
     tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg
     attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg
     ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca
     gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc
     atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg
     gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa
     ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg
     ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc
     gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac
     gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt
     tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa
     ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta
     agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat
     taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg
     attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc
     taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat
     acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta
     aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt
     gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac
     ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc
     cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg
     ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatgcg gtgtgaaata
     ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     cttcaaggaa ttgat
//