back Return to this vector's summary.
ID   PBRM       preliminary; circular DNA; SYN; 2014 BP.
XX
AC   ATCC37283;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBRM - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBRM from pBR322 & linker
RC   pSVE3 from SV40 & pBRM
RC   pSVELL9 from pSVE3 & SV40
RC   pSVL5 from pBRM & SV40 late region
RC   pHA 2-29 from pBR322 & virus A/WSN/33 HA gene
RC   SV-HA1, SV-HA2, SV-HA3, SV-HA4 from pBRM & pHA 2-29 & linker
RA   Hartman J.R., Nayak D.P., Fareed G.C.;
RT   "Human influenza virus hemagglutinin is expressed in monkey cells
RT   using simian virus 40 vectors";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:233-237(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   A 2 Kb derivative of pBR322.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBRM)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HaeII-EcoRI 2008bp 2352..4360,
FT                   \ ori/amp gene
FT                   \ HaeII = 237 418 439 499 553 780 1210 1649 1732 2354
FT                   \ 2724
FT                   blunt end:blunt end
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   remove small BamHI-BamHI 6bp
FT                   -> pBRM 2014bp"
FT   -               1..2008
FT                   /note="pBR322 2352..4359 2008bp
FT                   EcoRI = G^AATTC
FT                   BamHI =   G^GATCC"
FT   -               2009..2014
FT                   /note="ggatcc 6bp
FT                   BamHI = G^GATCC
FT                   HaeII =   RGCGC^Y"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2014 BP; 515 A; 510 C; 476 G; 513 T; 0 other;
     tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     cgtatcacga ggccctttcg tcttcaaggg atcc
//