back Return to this vector's summary.
ID   PBRS188    preliminary; circular DNA; SYN; 4349 BP.
XX
AC   K02384;
XX
DT   01-AUG-1985 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBRS188 - complete, no Tc-promoter.
XX
KW   cloning vector; drug resistance gene; mutational analysis;
KW   promoter region; tetracycline resistance.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBRS188 from pBR322
RC   pBRS206 from pBRS188 & pOP1, lambda immunity region
RA   Rechinskii V.O., Savochkina L.P., Bibilashvili R.S.;
RT   "[Construction of a plasmid vector for the cloning of promoters]";
RL   Mol. Biol. 15:950-956(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   derived from deletion of 12 bp of pBR322.
CC   [1] inserted strong promoters from T7 and lambda into pBR322
CC   derived promoter-probe vectors. The inserted promoters, which
CC   occurred in dissimilar environments, served as promoters for the
CC   pBR322 tetracycline resistance operon.  Promoter strength was
CC   measured by Tc resistance.  Plasmids containing T7 A2 promoters
CC   appeared to confer less Tc resistance than those containing A3
CC   promoters.
CC   NCBI gi: 209317
CC   NM (pBRS188)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(promoter analysis)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII 4361bp 30..30, tet promoter
FT                   DNA polymerase I with GTP
FT                   S1 nuclease, remove 12bp 27..38
FT                   -> pBRS188 4349bp [unique EcoRI]"
FT   -               1..26
FT                   /note="pBR322 1..26 26bp"
FT   -               27..4349
FT                   /note="pBR322 39..4361 4323bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 4349 BP; 978 A; 1209 C; 1133 G; 1029 T; 0 other;
     ttctcatgtt tgacagctta tcatcggcgg tagtttatca cagttaaatt gctaacgcag
     tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
     ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt
     ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca
     atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct
     gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct
     gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
     cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac
     ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
     gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
     accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
     aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
     gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     gtatcacgag gccctttcgt cttcaagaa
//