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ID   PBRS240    preliminary; circular DNA; SYN; 4367 BP.
XX
AC   K02385;
XX
DT   01-AUG-1985 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBRS240 - complete, pBR322 Tc-s phenotype.
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KW   cloning vector; drug resistance gene; mutational analysis;
KW   promoter region; tetracycline resistance.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-32
RC   pBRS206 from pBRS188 & pOP1, lambda immunity region
RC   pBRS242 from pBR322 & pBRS206
RC   pBRS240 from pBRS242
RC   pBRS246 from pBRS240
RC   pBRS2843, pBRS2852 from pBRS240
RC   pBRS2845, pBRS2846, pBRS2850 from pBRS240
RC   pBRS3077 from pBRS240
RC   pBRS2950 from pBRS240
RC   pBRS2849 from pBRS240
RC   pBRS2343 from pBRS188
RA   Savochkina L.P., Retchinsky V.O., Bibilashvili R.S.;
RT   "Stability of cloned promoter-containing fragments";
RL   Mol. Gen. Genet. 189:142-147(1983).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   [1] inserted strong promoters from T7 and lambda into pBR322
CC   derived promoter-probe vectors. The inserted promoters, which
CC   occurred in dissimilar environments, served as promoters for the
CC   pBR322 tetracycline resistance operon.  Promoter strength was
CC   measured by Tc resistance.  Plasmids containing T7 A2 promoters
CC   appeared to confer less Tc resistance than those containing A3
CC   promoters.
CC   NCBI gi: 208973
CC   NM (pBRS240)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(promoter analysis)
CC   SE ()
CC   PA (pBR322)(T7)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBRS242 remove EcoRI-EcoRI 99bp,
FT                   \ lacUV5 pro/4367bp
FT                   -> pBRS240 4367bp"
FT   -               1..26
FT                   /note="pBR322 1..26 26bp
FT                   ClaI = AT^CG AT
FT                   \            aatt"
FT   -               27..30
FT                   /note="aatt 4bp
FT                   \    aatt
FT                   ClaI = AT^CGAT"
FT   -               31..4367
FT                   /note="pBR322 25..4361 4337bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7 A2 or A3 promoter"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 4367 BP; 985 A; 1211 C; 1135 G; 1036 T; 0 other;
     ttctcatgtt tgacagctta tcatcgaatt cgataagctt taatgcggta gtttatcaca
     gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
     cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
     cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
     gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
     tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
     ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
     cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
     tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc
     atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg
     cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
     tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
     tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca
     gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
     acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg
     cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaa
//