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ID PBS preliminary; circular DNA; SYN; 2746 BP.
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AC M77811; IG0028;
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DT 06-DEC-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pBS - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pJF3 from pUC19 & T7 oligo
RC pBS or BlueScribe from pJF3 & T3 oligo
RC pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC pBluescript KS(+) from pBS+ & linker
RC pBluescript KS(-) from pBS- & linker
RC pBST-B from pBS & pEMBL8
RC pBSITO#12 from pBST-B & pEMBL8
RC pPreB from pBSITO#12 & pBluescript SK(-)
RC lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC lambda LongD from lambda LongC
RC lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT excision properties";
RL Nucleic Acids Res. 16:7583-7600(1988).
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RN [2]
RP 1-2746
RC pBS or BlueScribe
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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RN [3]
RC pBS or BlueScribe from pJF3 & T3 oligo
RA Fernandez J.M.;
RT ;
RL Unpublished (1991).
RL Fernandez J.M., Stratagene.
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CC Old name is BlueScribe.
CC NM (pBS)(BlueScribe)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Stratagene)
CC HO (E.coli JM101)(E.coli JM109)
CC CP ()
CC FN (cloning general)(transcription)(sequencing)
CC SE (color blue/white)
CC PA (pUC19)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC19 EcoRI 2686bp 397..397
FT dephosphorylation
FT 2. oligo EcoRI-EcoRI 30bp, T7 promoter
FT -> pJF3 2720bp
FT 1. pJF3 HindIII 2720bp, pUC19 448..448
FT dephosphorylation
FT 2. oligo HindIII-HindIII 30bp, T3 promoter
FT -> pBS 2746bp"
FT promoter 402..421
FT /note="PRO bacteriophage T7"
FT misc_binding 421..484
FT /note="MCS HindIII-SphI-PstI-BspMI-SalI-XbaI-
FT BamHI-SmaI-KpnI-SacI-EcoRI"
FT promoter complement(484..499)
FT /note="PRO bacteriophage T3"
FT promoter complement(653..745)
FT /note="PRO E. coli lac gene"
FT CDS complement(987..>987)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1686..2546)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 2746 BP; 680 A; 686 C; 700 G; 680 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
gcgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttt
tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//