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ID   PBS        preliminary; circular DNA; SYN; 2746 BP.
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AC   M77811; IG0028;
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DT   06-DEC-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBS - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJF3 from pUC19 & T7 oligo
RC   pBS or BlueScribe from pJF3 & T3 oligo
RC   pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC   pBluescript KS(+) from pBS+ & linker
RC   pBluescript KS(-) from pBS- & linker
RC   pBST-B from pBS & pEMBL8
RC   pBSITO#12 from pBST-B & pEMBL8
RC   pPreB from pBSITO#12 & pBluescript SK(-)
RC   lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC   lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC   lambda LongD from lambda LongC
RC   lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA   Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT   "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT   excision properties";
RL   Nucleic Acids Res. 16:7583-7600(1988).
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RN   [2]
RP   1-2746
RC   pBS or BlueScribe
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [3]
RC   pBS or BlueScribe from pJF3 & T3 oligo
RA   Fernandez J.M.;
RT   ;
RL   Unpublished (1991).
RL   Fernandez J.M., Stratagene.
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CC   Old name is BlueScribe.
CC   NM (pBS)(BlueScribe)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli JM101)(E.coli JM109)
CC   CP ()
CC   FN (cloning general)(transcription)(sequencing)
CC   SE (color blue/white)
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 EcoRI 2686bp 397..397
FT                   dephosphorylation
FT                   2. oligo EcoRI-EcoRI 30bp, T7 promoter
FT                   -> pJF3 2720bp
FT                   1. pJF3 HindIII 2720bp, pUC19 448..448
FT                   dephosphorylation
FT                   2. oligo HindIII-HindIII 30bp, T3 promoter
FT                   -> pBS 2746bp"
FT   promoter        402..421
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    421..484
FT                   /note="MCS HindIII-SphI-PstI-BspMI-SalI-XbaI-
FT                   BamHI-SmaI-KpnI-SacI-EcoRI"
FT   promoter        complement(484..499)
FT                   /note="PRO bacteriophage T3"
FT   promoter        complement(653..745)
FT                   /note="PRO E. coli lac gene"
FT   CDS             complement(987..>987)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1686..2546)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 2746 BP; 680 A; 686 C; 700 G; 680 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     gcgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttt
     tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//