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ID   PBS42      preliminary; circular DNA; SYN; 5636 BP.
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AC   ATCC37279;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/E.coli plasmid vector pBS42 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBS42 from pUB110 & pBR322 & pC194
RC   pSPIF series from pBS42 & spac-I [SPO-1 phage promoter] & interferon-A
RC   pSPIF-III from human leukocyte interferon A gene
RA   Band L., Henner D.J.;
RT   "Bacillus subtilis requires a stringent Shine-Dalgarno region
RT   for gene expression";
RL   DNA 3:17-21(1984).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   A Bacillus subtilis/E.coli shuttle vector.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pBS42)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(Bacillus subtilis)(E.coli)
CC   HO (Bacillus subtilis BD170)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUB110)(pC194)(pBR322)
CC   BR ()
CC   OF (pSPIF-I)(pSPIF-II)(pSPIF-III)(pSPIF-IV)(pSPIF-V)(pSPIF-VI)
CC   OF (pSPIF-VII)(pSPIF also from leukocyte interferon-A gene)
CC   OF (pSPIF also from spac-I from SPO-1 phage promoter, lac but no SD)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUB110 HpaII-BamHI 2600bp 1900..4500, origin
FT                   :blunt end
FT                   2. pBR322 PvuII-Sau3A/MboI 1157bp 2067..3224, origin
FT                   3. pC194 Sau3A/MboI-HpaII 1879bp 2005..2910..974, cat
FT                   -> pBS42 5636bp"
FT   -               1..2600
FT                   /note="pUB110 1900..4499 2600bp
FT                   BamHI =   G^GATCC
FT                   PvuII = CAG^CTG"
FT   -               2601..3757
FT                   /note="pBR322 2067..3223 1157bp
FT                   MboI = Sau3AI = ^GATC"
FT   -               3758..5636
FT                   /note="pC194 2005..2910..973 1879bp
FT                   HpaII = C^CGG"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="spac-I promoter"
FT   RBS             0..0
FT                   /note="RBS"
FT   CDS             0..0
FT                   /note="GEN leukocyte interferon A gene"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI/PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT Sau3A"
FT   CDS             0..0
FT                   /note="ANT Bacillus chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   misc_binding    0..0
FT                   /note="SIT HpaII"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
XX
SQ   Sequence 5636 BP; 1643 A; 1214 C; 874 G; 1905 T; 0 other;
     gtgaaaccta gtttatcgca ataaaaacct atactctttt taatatcccc gactggcaat
     gccgggatag actgtaacat tctcacgcat aaaatcccct ttcattttct aatgtaaatc
     tattacctta ttattaattc aattcgctca taattaatcc tttttcttat tacgcaaaat
     ggcccgattt aagcacaccc tttattccgt taatgcgcca tgacagccat gataattact
     aatactagga gaagttaata aatacgtaac caacatgatt aacaattatt agaggtcatc
     gttcaaaatg gtatgcgttt tgacacatcc actatatatc cgtgtcgttc tgtccactcc
     tgaatcccat tccagaaatt ctctagcgat tccagaagtt tctcagagtc ggaaagttga
     ccagacatta cgaactggca cagatggtca taacctgaag gaagatctga ttgcttaact
     gcttcagtta agaccgaagc gctcgtcgta taacagatgc gatgatgcag accaatcaac
     atggcacctg ccattgctac ctgtacagtc aaggatggta gaaatgttgt cggtccttgc
     acacgaatat tacgccattt gcctgcatat tcaaacagct cttctacgat aagggcacaa
     atcgcatcgt ggaacgtttg ggcttctacc gatttagcag tttgatacac tttctctaag
     tatccacctg aatcataaat cggcaaaata gagaaaaatt gaccatgtgt aagcggccaa
     tctgattcca cctgagatgc ataatctagt agaatctctt cgctatcaaa attcacttcc
     accttccact caccggttgt ccattcatgg ctgaactctg cttcctctgt tgacatgaca
     cacatcatct caatatccga atagggccca tcagtctgac gaccaagaga gccataaaca
     ccaatagcct taacatcatc cccatattta tccaatattc gttccttaat ttcatgaaca
     atcttcattc tttcttctct agtcattatt attggtccat tcactattct cattcccttt
     tcagataatt ttagatttgc ttttctaaat aagaatattt ggagagcacc gttcttattc
     agctattaat aactcgtctt cctaagcatc cttcaatcct tttaataaca attatagcat
     ctaatcttca acaaactggc ccgtttgttg aactactctt taataaaata atttttccgt
     tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc gtcatcatct
     gtatgaatca aatcgccttc ttctgtgtca tcaaggttta attttttatg tatttctttt
     aacaaaccac cataggagat taacctttta cggtgtaaac cttcctccaa atcagacaaa
     cgtttcaaat tcttttcttc atcatcggtc ataaaatccg tatcctttac aggatatttt
     gcagtttcgt caattgccga ttgtatatcc gatttatatt tatttttcgg tcgaatcatt
     tgaactttta catttggatc atagtctaat ttcattgcct ttttccaaaa ttgaatccat
     tgtttttgat tcacgtagtt ttctgtattc ttaaaataag ttggttccac acataccaat
     acatgcatgt gctgattata agaattatct ttattattta ttgtcacttc cgttgcacgc
     ataaaaccaa caagattttt attaattttt ttatattgca tcattcggcg aaatccttga
     gccatatctg acaaactctt atttaattct tcgccatcat aaacattttt aactgttaat
     gtgagaaaca accaacgaac tgttggcttt tgtttaataa cttcagcaac aaccttttgt
     gactgaatgc catgtttcat tgctctcctc cagttgcaca ttggacaaag cctggattta
     caaaaccaca ctcgatacaa ctttctttcg cctgtttcac gattttgttt atactctaat
     atttcagcac aatcttttac tctttcagcc tttttaaatt caagaatatg cagaagttca
     aagtaatcaa cattagcgat tttcttttct ctccatggtc tcacttttcc actttttgtc
     ttgtccacta aaacccttga tttttcatct gaataaatgc tactattagg acacataata
     ttaaaagaaa cccccatcta tttagttatt tgtttagtca cttataactt taacagatgg
     ggtttttctg tgcaaccaat tttaagggtt ttcaatactt taaaacacat acataccaac
     acttcaacgc acctttcagc aactaaaata aaaatgacgt tatttctata tgtatcaaga
     taagaaagaa caagttcaaa accatcaaaa aaagacacct tttcaggtgc tttttttatt
     ttataaactc attccctgat ctcgacttcg ttcttttttt acctctcggt tatgagttag
     ttcaaattcg ttctttttag gttctaaatc gtgtttttct tggaattgtg ctgttttatc
     ctttaccttg tctacaaacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     ttttggtcat gagattatca aaaaggatct tcacctagat ccatatcctt ctttttctga
     accgacttct cctttttcgc ttctttattc caattgcttt attgacgttg agcctcggaa
     cccttaacaa tcccaaaact tgtcgaatgg tcggcttaat agctcacgct atgccgacat
     tcgtctgcaa gtttagttaa gggttcttct caacgcacaa taaattttct cggcataaat
     gcgtggtcta atttttattt ttaataacct tgatagcaaa aaatgccatt ccaatacaaa
     accacatacc tataatcgat aaccacataa cagtcataaa accactcctt tttaacaaac
     tttatcacaa gaaatattta aattttaaat gcctttattt tgaattttaa ggggcatttt
     aaagatttag gggtaaatca tatagtttta tgcctaaaaa cctacagaag cttttaaaaa
     gcaaatatga gccaaataaa tatattctaa ttctacaaac aaaaatttga gcaaattcag
     tgtcgatttt ttaagacact gcccagttac atgcaaatta aaattttcat gattttttat
     agttcctaac agggttaaaa tttgtataac gaaagtataa tgtttatata acgttagtat
     aataaagcat tttaacatta tacttttgat aatcgtttat cgtcgtcatc acaataactt
     ttaaaatact cgtgcataat tcacgctgac ctcccaataa ctacatggtg ttatcgggag
     gtcagctgtt agcacttata ttttgttatt gttcttcctc gatttcgtct atcattttgt
     gattaatttc tcttttttct tgttctgtta agtcataaag ttcactagct aaatactctt
     tttgtttcca aatataaaaa atttgataga tatattacgg ttggatcaat ttcttttaag
     taatctaaat ccccattttt taatttcttt ttagcctctt taaataatcc tgaataaact
     aatacctgtt tacctttaag tgatttataa aatgcatcaa agactttttg atttatttat
     taaataatca ctatctttac cagaatactt agccatttca tataattctt tattattatt
     ttgtcttatt ttttgaactt gaacttgtgt tatttctgaa atgcccgtta catcacgcca
     taaatctaac cattcttgtt ggctaatata atatctttta tctgtgaaat acgatttatt
     tactgcaatt aacacatgaa aatgaggatt ataatcatct ctttttttat tatatgtaat
     ctctaactta cgaacatatc cctttataac actacctact ttttttctct ttataagttt
     tctaaaagaa ttattataac gttttatttc attttctaat tcatcactca ttacattagg
     tgtagtcaaa gttaaaaaga taaactcctt tttctcttgc tgcttaatat attgcatcat
     caaagataaa cccaatgcat cttttctagc ttttctccaa gcacagacag gacaaaatcg
     atttttacaa gaattagctt tatataattt ctgtttttct aaagttttat cagctacaaa
     agacagaaat gtattgcaat cttcaactaa atccatttga ttctctccaa tatgacgttt
     aataaatttc tgaaatactt gatttctttg ttttttctca gtatactttt ccatgttata
     acacataaaa acaacttagt tttcacaaac tatgacaata aaaaaagttg ctttttcccc
     tttctatgta tgttttttac tagtcattta aaacgataca ttaataggta cgaaaaagca
     actttttttg cgcttaaaac cagtcatacc aataacttaa gggtaactag cctcgc
//