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ID PBS7 preliminary; circular DNA; SYN; 6104 BP.
XX
AC K01433; ATCC37280;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Bacillus/E.coli plasmid vector pBS7 - complete.
XX
KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pIQ2 from pBS42 & pHiQ6
RC pBSA42 from pBSA105
RC pIQ45 from pBSA42 & pIQ2 & pHiQ6
RC pAIQ25 from pBSA105 & pBS42 & pIQ45
RC pBS7 from pUB110 & pBR322
RC pAIQ120 from pAIQ25 & pBS7
RC pLIQ-1 from pAIQ120 & pSPIF-III
RA Yansura D.G., Henner D.J.;
RT "Use of the Escherichia coli lac repressor and operator to control
RT gene expression in Bacillus subtilis";
RL Proc. Natl. Acad. Sci. U.S.A. 81:439-443(1984).
XX
RN [2]
RC pBSA105 from SPO-1 phage promoter, lac but no SD
RA Yansura D.G., Henner D.J.;
RT "Development of an inducible promoter system for Bacillus subtilis";
RL Biology and Biotechnology of the Bacilli 0:0-0(1983).
RL Ganesan A.T., Hoch J.A., eds., Academic, New York.
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RN [3]
RC [lambda h80dlac from lambda & lacI repressor gene]
RC pHI series from pMB9 & lambda h80dlac, lacI repressor gene
RC pHiQ3, pHiQ6 from pMB9 & lambda h80dlac, lacI gene/lacIq gene
RA Hare D.L., Sadler J.R.;
RT "Cloning of the lacIq gene into a ColE1 plasmid";
RL Gene 3:269-278(1978).
XX
RN [4]
RC pHO2 from pBR322 & lactose operator
RA Sadler J.R., Tecklenberg M., Betz J.L., Goeddel D.V., Yansura D.G.,
RA Caruthers M.H.;
RT "Cloning of chemically synthesized lactose operators";
RL Gene 1:305-321(1977).
XX
RN [5]
RC pOE10, pOE12 from pMB9 & lactose operator
RC pOE38, pOE39, pOE40 from pMB9 & pOE10, lac operator
RA Sadler J.R., Betz J.L., Tecklenberg M., Goeddel D.V., Yansura D.G.,
RA Caruthers M.H.;
RT "Cloning of chemically synthesized lactose operators, II.
RT EcoRI-linkered operators";
RL Gene 3:211-232(1978).
XX
RN [6]
RC pHGH207-1 from trp promoter
RC pHGH107-11 from lac promoter
RA De Boer H.A., Comstock L.J., Hui A., Wong E., Vasser M.;
RT "Portable Shine-Dalgarno regions: A system for a systematic study
RT of defined alterations of nucleotide sequences within E. coli ribosome
RT binding sites";
RL DNA 2:231-235(1983).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC A Bacillus subtilis/E.coli shuttle vector.
CC Medium is 1065 LB medium.
CC NM (pBS7)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli MM294)(Bacillus subtilis)(E.coli)
CC HO (Bacillus subtilis BD170)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUB110)(pBR322)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUB110 BamHI 4548bp 4500..4500, 5' to neo
FT 2. pBR322 Sau3A/MboI-Sau3A/MboI 1556bp 1668..3224, ori
FT -> pBS7 6104bp"
FT - 1..4499
FT /note="pUB110 1..4499 4499bp
FT BamHI = G^GATCC
FT MboI = Sau3AI = ^GATC"
FT - 4500..6055
FT /note="pBR322 1668..3223 1556bp
FT MboI = Sau3AI = ^GATC
FT BamHI = G^GATCC"
FT - 6056..6104
FT /note="pUB110 4500..4548 49bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-XbaI-PvuII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI Bacillus pUB110"
FT CDS 0..0
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT); neomycin resistance gene (neo)"
XX
SQ Sequence 6104 BP; 1682 A; 1429 C; 1030 G; 1963 T; 0 other;
gaattcctta gtgctttcat agattaaact cacatcacgc tttaaatcgc ttattttaga
ctttaaagac ttgttttctt caagcaactc attataatca tttacatttt cattaaatcg
ctctacaaga ccactatatt tttctttaac ttgcccatgt tctttactta attttttata
ttctctcgcc atatcagtac tcatgagatt tctaacatgc tgttttaacc tatcgttatc
tctcgcagca gtcactaagt ttttataatc acgctccgat ataacaacat ttttggttgg
tttcttttct gttttcatta tttcttttcc caaaccaaac atggactttt cacccgttgg
cacttcaaca cttttcatgt gtcgtttcgc tggtacttct aaatctgatt taactttatc
gctataagca gtccattcat cttttttaac tgctaaattt ttttctagaa aatcaatctc
tttttccaaa gtttgttttt taaatttagc tgtctcaata tgtttacggt cagagccacg
ttcaccacgc ttcaactcaa aaccctgttt tttcatatgc tcggggaatt tatcttgtag
ccataacagt tcttgacgat taaacacatt ttttccttgc agttttccat cacgcatagg
cacaacacct aaatgcatgt gaggggtttg ctcatcatta tgaactgttg cataagcaat
attttgcttg ccatatcgtt cggaaaataa tttataactt tcctcaaaaa atcgtttttg
ttctcctgga tccagttgct caaaaaaatc tcggtcagat gttactagca actcatttac
aagaacagca tctttcctcc gtttttcttg tacctgtttt ttgtgattca ataatttctt
tgacacgttc gttgtaatca atatttttat catttttcaa atcataattt tcacgtgttc
gctcatggtc aatatcatca ttcgttctac tttttcgctc tctttgatta tgaaattgca
tgccttttag tccagctgat ttcacttttt gcattctaca aactgcataa ctcatatgta
aatcgctcct ttttaggtgg cacaaatgtg aggcattttc gctctttccg gcaaccactt
ccaagtaaag tataacacac tatactttat attcataaag tgtgtgctct gcgaggctgt
cggcagtgcc gaccaaaacc ataaaacctt taagaccttt ctttttttta cgagaaaaaa
gaaacaaaaa aacctgccct ctgccacctc agcaaagggg ggttttgctc tcgtgctcgt
ttaaaaatca gcaagggaca ggtagtattt tttgagaaga tcactcaaaa aatctccacc
tttaaaccct tgccaatttt tattttgtcc gttttgtcta gcttaccgaa agccagactc
agcaagaata aaatttttat tgtctttcgg ttttctagtg taacggacaa aaccactcaa
aataaaaaag atacaagaga ggtctctcgt atcttttatt cagcaatcgc gcccgattgc
tgaacagatt aataatagat tttagctttt tatttgttga aaaaagctaa tcaaattgtt
gtcgggatca attactgcaa agtctcgttc atcccaccac tgatctttta atgatgtatt
ggggtgcaaa atgcccaaag gcttaatatg ttgatataat tcatcaattc cctctacttc
aatgcggcaa ctagcagtac cagcaataaa cgactccgca cctgtacaaa ccggtgaatc
attactacga gagcgccagc ttcatcactt gcctcccata gatgaatccg aacctcatta
cacattagaa ctgcgaatcc atcttcatgg tgaaccaaag tgaaacctag tttatcgcaa
taaaaaccta tactcttttt aatatccccg actggcaatg ccgggataga ctgtaacatt
ctcacgcata aaatcccctt tcattttcta atgtaaatct attaccttat tattaattca
attcgctcat aattaatcct ttttcttatt acgcaaaatg gcccgattta agcacaccct
ttattccgtt aatgcgccat gacagccatg ataattacta atactaggag aagttaataa
atacgtaacc aacatgatta acaattatta gaggtcatcg ttcaaaatgg tatgcgtttt
gacacatcca ctatatatcc gtgtcgttct gtccactcct gaatcccatt ccagaaattc
tctagcgatt ccagaagttt ctcagagtcg gaaagttgac cagacattac gaactggcac
agatggtcat aacctgaagg aagatctgat tgcttaactg cttcagttaa gaccgaagcg
ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca tggcacctgc cattgctacc
tgtacagtca aggatggtag aaatgttgtc ggtccttgca cacgaatatt acgccatttg
cctgcatatt caaacagctc ttctacgata agggcacaaa tcgcatcgtg gaacgtttgg
gcttctaccg atttagcagt ttgatacact ttctctaagt atccacctga atcataaatc
ggcaaaatag agaaaaattg accatgtgta agcggccaat ctgattccac ctgagatgca
taatctagta gaatctcttc gctatcaaaa ttcacttcca ccttccactc accggttgtc
cattcatggc tgaactctgc ttcctctgtt gacatgacac acatcatctc aatatccgaa
tagggcccat cagtctgacg accaagagag ccataaacac caatagcctt aacatcatcc
ccatatttat ccaatattcg ttccttaatt tcatgaacaa tcttcattct ttcttctcta
gtcattatta ttggtccatt cactattctc attccctttt cagataattt tagatttgct
tttctaaata agaatatttg gagagcaccg ttcttattca gctattaata actcgtcttc
ctaagcatcc ttcaatcctt ttaataacaa ttatagcatc taatcttcaa caaactggcc
cgtttgttga actactcttt aataaaataa tttttccgtt cccaattcca cattgcaata
atagaaaatc catcttcatc ggctttttcg tcatcatctg tatgaatcaa atcgccttct
tctgtgtcat caaggtttaa ttttttatgt atttctttta acaaaccacc ataggagatt
aaccttttac ggtgtaaacc ttcctccaaa tcagacaaac gtttcaaatt cttttcttca
tcatcggtca taaaatccgt atcctttaca ggatattttg cagtttcgtc aattgccgat
tgtatatccg atttatattt atttttcggt cgaatcattt gaacttttac atttggatca
tagtctaatt tcattgcctt tttccaaaat tgaatccatt gtttttgatt cacgtagttt
tctgtattct taaaataagt tggttccaca cataccaata catgcatgtg ctgattataa
gaattatctt tattatttat tgtcacttcc gttgcacgca taaaaccaac aagattttta
ttaatttttt tatattgcat cattcggcga aatccttgag ccatatctga caaactctta
tttaattctt cgccatcata aacattttta actgttaatg tgagaaacaa ccaacgaact
gttggctttt gtttaataac ttcagcaaca accttttgtg actgaatgcc atgtttcatt
gctctcctcc agttgcacat tggacaaagc ctggatttac aaaaccacac tcgatacaac
tttctttcgc ctgtttcacg attttgttta tactctaata tttcagcaca atcttttact
ctttcagcct ttttaaattc aagaatatgc agaagttcaa agtaatcaac attagcgatt
ttcttttctc tccatggtct cacttttcca ctttttgtct tgtccactaa aacccttgat
ttttcatctg aataaatgct actattagga cacataatat taaaagaaac ccccatctat
ttagttattt gtttagtcac ttataacttt aacagatggg gtttttctgt gcaaccaatt
ttaagggttt tcaatacttt aaaacacata cataccaaca cttcaacgca cctttcagca
actaaaataa aaatgacgtt atttctatat gtatcaagat aagaaagaac aagttcaaaa
ccatcaaaaa aagacacctt ttcaggtgct ttttttattt tataaactca ttccctgatc
tcgacttcgt tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg
ttctaaatcg tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccg
atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag
cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt
ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc
atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag
gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctacctta
aaaacgtttt taaaggcttt taagccgtct gtacgttcct taag
//