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ID   PBS7       preliminary; circular DNA; SYN; 6104 BP.
XX
AC   K01433; ATCC37280;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/E.coli plasmid vector pBS7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pIQ2 from pBS42 & pHiQ6
RC   pBSA42 from pBSA105
RC   pIQ45 from pBSA42 & pIQ2 & pHiQ6
RC   pAIQ25 from pBSA105 & pBS42 & pIQ45
RC   pBS7 from pUB110 & pBR322
RC   pAIQ120 from pAIQ25 & pBS7
RC   pLIQ-1 from pAIQ120 & pSPIF-III
RA   Yansura D.G., Henner D.J.;
RT   "Use of the Escherichia coli lac repressor and operator to control
RT   gene expression in Bacillus subtilis";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:439-443(1984).
XX
RN   [2]
RC   pBSA105 from SPO-1 phage promoter, lac but no SD
RA   Yansura D.G., Henner D.J.;
RT   "Development of an inducible promoter system for Bacillus subtilis";
RL   Biology and Biotechnology of the Bacilli 0:0-0(1983).
RL   Ganesan A.T., Hoch J.A., eds., Academic, New York.
XX
RN   [3]
RC   [lambda h80dlac from lambda & lacI repressor gene]
RC   pHI series from pMB9 & lambda h80dlac, lacI repressor gene
RC   pHiQ3, pHiQ6 from pMB9 & lambda h80dlac, lacI gene/lacIq gene
RA   Hare D.L., Sadler J.R.;
RT   "Cloning of the lacIq gene into a ColE1 plasmid";
RL   Gene 3:269-278(1978).
XX
RN   [4]
RC   pHO2 from pBR322 & lactose operator
RA   Sadler J.R., Tecklenberg M., Betz J.L., Goeddel D.V., Yansura D.G.,
RA   Caruthers M.H.;
RT   "Cloning of chemically synthesized lactose operators";
RL   Gene 1:305-321(1977).
XX
RN   [5]
RC   pOE10, pOE12 from pMB9 & lactose operator
RC   pOE38, pOE39, pOE40 from pMB9 & pOE10, lac operator
RA   Sadler J.R., Betz J.L., Tecklenberg M., Goeddel D.V., Yansura D.G.,
RA   Caruthers M.H.;
RT   "Cloning of chemically synthesized lactose operators, II.
RT   EcoRI-linkered operators";
RL   Gene 3:211-232(1978).
XX
RN   [6]
RC   pHGH207-1 from trp promoter
RC   pHGH107-11 from lac promoter
RA   De Boer H.A., Comstock L.J., Hui A., Wong E., Vasser M.;
RT   "Portable Shine-Dalgarno regions: A system for a systematic study
RT   of defined alterations of nucleotide sequences within E. coli ribosome
RT   binding sites";
RL   DNA 2:231-235(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   A Bacillus subtilis/E.coli shuttle vector.
CC   Medium is 1065 LB medium.
CC   NM (pBS7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(Bacillus subtilis)(E.coli)
CC   HO (Bacillus subtilis BD170)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUB110)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUB110 BamHI 4548bp 4500..4500, 5' to neo
FT                   2. pBR322 Sau3A/MboI-Sau3A/MboI 1556bp 1668..3224, ori
FT                   -> pBS7 6104bp"
FT   -               1..4499
FT                   /note="pUB110 1..4499 4499bp
FT                   BamHI =        G^GATCC
FT                   MboI = Sau3AI = ^GATC"
FT   -               4500..6055
FT                   /note="pBR322 1668..3223 1556bp
FT                   MboI = Sau3AI = ^GATC
FT                   BamHI =        G^GATCC"
FT   -               6056..6104
FT                   /note="pUB110 4500..4548 49bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-XbaI-PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
XX
SQ   Sequence 6104 BP; 1682 A; 1429 C; 1030 G; 1963 T; 0 other;
     gaattcctta gtgctttcat agattaaact cacatcacgc tttaaatcgc ttattttaga
     ctttaaagac ttgttttctt caagcaactc attataatca tttacatttt cattaaatcg
     ctctacaaga ccactatatt tttctttaac ttgcccatgt tctttactta attttttata
     ttctctcgcc atatcagtac tcatgagatt tctaacatgc tgttttaacc tatcgttatc
     tctcgcagca gtcactaagt ttttataatc acgctccgat ataacaacat ttttggttgg
     tttcttttct gttttcatta tttcttttcc caaaccaaac atggactttt cacccgttgg
     cacttcaaca cttttcatgt gtcgtttcgc tggtacttct aaatctgatt taactttatc
     gctataagca gtccattcat cttttttaac tgctaaattt ttttctagaa aatcaatctc
     tttttccaaa gtttgttttt taaatttagc tgtctcaata tgtttacggt cagagccacg
     ttcaccacgc ttcaactcaa aaccctgttt tttcatatgc tcggggaatt tatcttgtag
     ccataacagt tcttgacgat taaacacatt ttttccttgc agttttccat cacgcatagg
     cacaacacct aaatgcatgt gaggggtttg ctcatcatta tgaactgttg cataagcaat
     attttgcttg ccatatcgtt cggaaaataa tttataactt tcctcaaaaa atcgtttttg
     ttctcctgga tccagttgct caaaaaaatc tcggtcagat gttactagca actcatttac
     aagaacagca tctttcctcc gtttttcttg tacctgtttt ttgtgattca ataatttctt
     tgacacgttc gttgtaatca atatttttat catttttcaa atcataattt tcacgtgttc
     gctcatggtc aatatcatca ttcgttctac tttttcgctc tctttgatta tgaaattgca
     tgccttttag tccagctgat ttcacttttt gcattctaca aactgcataa ctcatatgta
     aatcgctcct ttttaggtgg cacaaatgtg aggcattttc gctctttccg gcaaccactt
     ccaagtaaag tataacacac tatactttat attcataaag tgtgtgctct gcgaggctgt
     cggcagtgcc gaccaaaacc ataaaacctt taagaccttt ctttttttta cgagaaaaaa
     gaaacaaaaa aacctgccct ctgccacctc agcaaagggg ggttttgctc tcgtgctcgt
     ttaaaaatca gcaagggaca ggtagtattt tttgagaaga tcactcaaaa aatctccacc
     tttaaaccct tgccaatttt tattttgtcc gttttgtcta gcttaccgaa agccagactc
     agcaagaata aaatttttat tgtctttcgg ttttctagtg taacggacaa aaccactcaa
     aataaaaaag atacaagaga ggtctctcgt atcttttatt cagcaatcgc gcccgattgc
     tgaacagatt aataatagat tttagctttt tatttgttga aaaaagctaa tcaaattgtt
     gtcgggatca attactgcaa agtctcgttc atcccaccac tgatctttta atgatgtatt
     ggggtgcaaa atgcccaaag gcttaatatg ttgatataat tcatcaattc cctctacttc
     aatgcggcaa ctagcagtac cagcaataaa cgactccgca cctgtacaaa ccggtgaatc
     attactacga gagcgccagc ttcatcactt gcctcccata gatgaatccg aacctcatta
     cacattagaa ctgcgaatcc atcttcatgg tgaaccaaag tgaaacctag tttatcgcaa
     taaaaaccta tactcttttt aatatccccg actggcaatg ccgggataga ctgtaacatt
     ctcacgcata aaatcccctt tcattttcta atgtaaatct attaccttat tattaattca
     attcgctcat aattaatcct ttttcttatt acgcaaaatg gcccgattta agcacaccct
     ttattccgtt aatgcgccat gacagccatg ataattacta atactaggag aagttaataa
     atacgtaacc aacatgatta acaattatta gaggtcatcg ttcaaaatgg tatgcgtttt
     gacacatcca ctatatatcc gtgtcgttct gtccactcct gaatcccatt ccagaaattc
     tctagcgatt ccagaagttt ctcagagtcg gaaagttgac cagacattac gaactggcac
     agatggtcat aacctgaagg aagatctgat tgcttaactg cttcagttaa gaccgaagcg
     ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca tggcacctgc cattgctacc
     tgtacagtca aggatggtag aaatgttgtc ggtccttgca cacgaatatt acgccatttg
     cctgcatatt caaacagctc ttctacgata agggcacaaa tcgcatcgtg gaacgtttgg
     gcttctaccg atttagcagt ttgatacact ttctctaagt atccacctga atcataaatc
     ggcaaaatag agaaaaattg accatgtgta agcggccaat ctgattccac ctgagatgca
     taatctagta gaatctcttc gctatcaaaa ttcacttcca ccttccactc accggttgtc
     cattcatggc tgaactctgc ttcctctgtt gacatgacac acatcatctc aatatccgaa
     tagggcccat cagtctgacg accaagagag ccataaacac caatagcctt aacatcatcc
     ccatatttat ccaatattcg ttccttaatt tcatgaacaa tcttcattct ttcttctcta
     gtcattatta ttggtccatt cactattctc attccctttt cagataattt tagatttgct
     tttctaaata agaatatttg gagagcaccg ttcttattca gctattaata actcgtcttc
     ctaagcatcc ttcaatcctt ttaataacaa ttatagcatc taatcttcaa caaactggcc
     cgtttgttga actactcttt aataaaataa tttttccgtt cccaattcca cattgcaata
     atagaaaatc catcttcatc ggctttttcg tcatcatctg tatgaatcaa atcgccttct
     tctgtgtcat caaggtttaa ttttttatgt atttctttta acaaaccacc ataggagatt
     aaccttttac ggtgtaaacc ttcctccaaa tcagacaaac gtttcaaatt cttttcttca
     tcatcggtca taaaatccgt atcctttaca ggatattttg cagtttcgtc aattgccgat
     tgtatatccg atttatattt atttttcggt cgaatcattt gaacttttac atttggatca
     tagtctaatt tcattgcctt tttccaaaat tgaatccatt gtttttgatt cacgtagttt
     tctgtattct taaaataagt tggttccaca cataccaata catgcatgtg ctgattataa
     gaattatctt tattatttat tgtcacttcc gttgcacgca taaaaccaac aagattttta
     ttaatttttt tatattgcat cattcggcga aatccttgag ccatatctga caaactctta
     tttaattctt cgccatcata aacattttta actgttaatg tgagaaacaa ccaacgaact
     gttggctttt gtttaataac ttcagcaaca accttttgtg actgaatgcc atgtttcatt
     gctctcctcc agttgcacat tggacaaagc ctggatttac aaaaccacac tcgatacaac
     tttctttcgc ctgtttcacg attttgttta tactctaata tttcagcaca atcttttact
     ctttcagcct ttttaaattc aagaatatgc agaagttcaa agtaatcaac attagcgatt
     ttcttttctc tccatggtct cacttttcca ctttttgtct tgtccactaa aacccttgat
     ttttcatctg aataaatgct actattagga cacataatat taaaagaaac ccccatctat
     ttagttattt gtttagtcac ttataacttt aacagatggg gtttttctgt gcaaccaatt
     ttaagggttt tcaatacttt aaaacacata cataccaaca cttcaacgca cctttcagca
     actaaaataa aaatgacgtt atttctatat gtatcaagat aagaaagaac aagttcaaaa
     ccatcaaaaa aagacacctt ttcaggtgct ttttttattt tataaactca ttccctgatc
     tcgacttcgt tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg
     ttctaaatcg tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccg
     atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag
     cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt
     ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc
     atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag
     gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
     gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
     gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctacctta
     aaaacgtttt taaaggcttt taagccgtct gtacgttcct taag
//