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ID   PBSM       preliminary; circular DNA; SYN; 3204 BP.
XX
AC   IG0026;
XX
DT   11-DEC-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pBS- - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBS- from pUC19
RA   ;
RT   ;
RL   Unpublished (1991).
XX
CC   The rescued strand will contain the non-coding strand
CC   of the beta-galactosidase (lacZ) gene.
CC   NM (pBS-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli JM101)(E.coli JM109)
CC   CP ()
CC   FN (cloning)(transcription)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBS NarI 2746bp 237..237
FT                   mung bean nuclease
FT                   alkaline phosphatase
FT                   2. pEMBL8- DraI-RsaI 456bp 897..1353,
FT                   \ f1 ori/no gene II
FT                   blunt end:blunt end
FT                   -> pBS- 3204bp"
FT   rep_origin      complement(235..689)
FT                   /note="ORI bacteriophage f1 intergenic region,
FT                   RsaI-DraI fragment (454 bp) in NarI site"
FT   promoter        complement(860..879)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    879..942
FT                   /note="MCS HindIII-SphI-PstI-BspMI-SalI-XbaI-
FT                   BamHI-SmaI-KpnI-SacI-EcoRI"
FT   promoter        complement(942..957)
FT                   /note="PRO bacteriophage T3"
FT   misc_feature    complement(987..987)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        complement(1110..1203)
FT                   /note="PRO E. coli lac gene"
FT   rep_origin      1379..1379
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2144..3044)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3204 BP; 776 A; 808 C; 802 G; 818 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgac
     gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     gcgaatttta acaaaatatt aacgtttaca atttcgccat tcgccattca ggctgcgcaa
     ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg
     gggatcctct agagtcgacc tgcaggcatg caagcttttg ttccctttag tgagggttaa
     ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     ggcgtatcac gaggcccttt cgtc
//