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ID   PBTAC1     preliminary; circular DNA; SYN; 4590 BP.
XX
AC   IG0163; VB0070;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBTac1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKM-1 from pKO-1
RC   pPCV from pBR322 & linker
RC   pBR322tacI from pHGH207-1 & pHGH107-11 & pBR322
RC   pHGH807tacI from pBR322tacI & pHGH207-1
RC   pHGH907tacII from pBR322tacI & pHGH207-1
RC   pKM-lac from pKM-1 & pHGH107-11
RC   pKM-trp from pKM-1 & pHGH207-1
RC   pKM-tacI from pKM-1 & pHGH807tacI
RC   pKM-tacII from pKM-1 & pHGH907tacII
RA   De Boer H.A., Comstock L.J., Vasser M.;
RT   "The tac promoter: a functional hybrid derived from the trp and
RT   lac promoters";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:21-25(1983).
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RN   [2]
RC   from lambda rifd18
RC   pKK34-121 from pBR322 & E.coli rrnB promoter P1
RC   pKK35-120 from pKT21 & E.coli rrnB promoter P1
RA   Brosius J., Dull T.J., Sleeter D.D., Noller H.F.;
RT   "Gene organization and primary structure of a ribosomal RNA operon
RT   from Escherichia coli";
RL   J. Mol. Biol. 148:107-127(1981).
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RN   [3]
RC   pHGH107 from pBR322 & lacUV5 promoter & human growth hormone gene
RC   pBR322-trp from pBR322 & trp promoter
RC   pHGH207-1 from pBR322-trp & pHGH107-11, human growth hormone gene
RC   pHGH107-11 from pHGH207-1 & pHGH107
RC   pHGHtrplac from pHGH207-1 & pHGH107
RC   pBR322-tac from pBR322 & tac promoter
RC   pHGH807tac from pBR322-tac & pHGH207-1
RA   De Boer H.A., Comstock L.J., Yansura D., Heyneker H.;
RT   "Construction of a tandem trp-lac promoter and a hybrid trp-lac
RT   promoter for efficient and controlled expression of the human growth
RT   hormone gene in Escherichia coli";
RL   Promoters Structure and Function 0:462-481(1982).
RL   Rodriguez R.L., Chamberlin M.J., Praeger, New York.
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RN   [4]
RC   pHGH107-11 from lacUV5 promoter
RA   De Boer H.A., Heyneker H., Comstock L.J., Wieland A., Vasser M.,
RA   Horn T.;
RT   ;
RL   Miami Winter Symposium [From Gene to Protein] 20:0-0(1983).
RL   Ahmad F., Schultz J., Smith E.E., Whelan W.J., Academic, New York.
XX
CC   Size is 4585 in Boehringer.
CC   lac repressor regulates tac promoter.
CC   old Boehringer vector.
CC   NM (pBTac1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli JM101)(E.coli JM105)(E.coli JM107)(E.coli JM109)
CC   CP ()
CC   FN (expression regulated)
CC   SE ()
CC   PA (pKK223-3)(pBR322)
CC   BR ()
CC   OF (pBTac2)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 3872bp 375..4247
FT                   2. pKK223-3 BamHI 4584bp
FT                   fill in
FT                   remove, 4584bp-
FT                   3. pUC8, MCS
FT                   -> pBTac1 4590bp"
FT   promoter        complement(1..250)
FT                   /note="PRO E. coli tac promoter cartridge"
FT   promoter        complement(3..74)
FT                   /note="PRO E. coli tac promoter (trp -35 region and
FT                   lacUV5 -10 region)"
FT   misc_feature    257..4129
FT                   /note="from pBR322 bases 375..4247"
FT   misc_feature    258..261
FT                   /note="SIT BamHI site of pKK223-3 filled in"
FT   CDS             >257..<1500
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    260..260
FT                   /note="SIT unique ClaI"
FT   misc_binding    452..452
FT                   /note="SIT unique SphI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             3180..3968
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3624..3624
FT                   /note="SIT unique PvuI"
FT   terminator      4129..4553
FT                   /note="TER E. coli rrnB terminator T1, T2, and 5S
FT                   from EcoRGNB bases complement(6416..6840)"
FT   misc_binding    4558..4588
FT                   /note="MCS unique HindIII-PstI-SalI-BamHI-SmaI-EcoRI
FT                   from M13mp8/pUC8 polylinker"
XX
SQ   Sequence 4590 BP; 1044 A; 1268 C; 1193 G; 1085 T; 0 other;
     ttctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagccgatg
     attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa
     aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac
     ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
     gcagtcgata agctccggat cgatcctcta cgccggacgc atcgtggccg gcatcaccgg
     cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
     tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc
     atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg
     cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
     tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
     tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca cggaggcatc
     aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
     cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg
     gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaaagagttt
     gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttaatttga tgcctggcag
     tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg ttcaaatccg
     ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg
     gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat tctgttttat cagaccgctt
     ctgcgttctg atttaatctg tatcaggctg aaaatcttct ctcatccgcc aaaacagaag
     cttggctgca ggtcgacgga tccccgggaa
//