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ID   PC194      preliminary; circular DNA; PRO; 2910 BP.
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AC   V01277; J01754; ATCC37034;
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DT   15-MAR-1985 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pC194 - complete.
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KW   cloning vector.
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OS   Staphylococcus aureus
OC   Prokaryota; Bacteria; Firmicutes; Gram-positive cocci; Micrococcaceae;
OC   Staphylococcus.
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RN   [1]
RP   1-2910
RC   pHW9 from pBR322 & pC194
RC   pHW10 from pBR322 & pC194
RC   pHW11 from pBR322 & pC194
RC   pHW12 from pBR322 & pC194
RC   pHW13 from pBR322 & pC194
RA   Horinouchi S., Weisblum B.;
RT   "Nucleotide sequence and functional map of pc194, a plasmid that
RT   specifies inducible chloramphenicol resistance";
RL   J. Bacteriol. 150:815-825(1982).
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RN   [2]
RC   pSC194 or pSA2100 from pC194 & pSA0501
RC   pSA2101 from pSA2100
RC   pSC194 str-4 or pSA2107 from pSC194 or pSA2100
RC   pCM194 from pC194
RA   Iordanescu S.;
RT   "Recombinant plasmid obtained from two different, compatible
RT   staphylococcal plasmids";
RL   J. Bacteriol. 124:597-601(1975).
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RN   [3]
RC   pC194, pS194 from Staphylococcus
RC   pSA0501 or pS194 seg-I from pS194
RA   Iordanescu S., Greceanu V.;
RT   "Localisation du determinant de la streptomycino-resistance chez
RT   une souche de Staphylococcus aureus";
RL   Arch. Roum. Pathol. Exp. Microbiol. 30:463-471(1971).
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RN   [4]
RC   pWL7 from amp gene & kan gene
RA   Goebel W.;
RT   ;
RL   Unpublished (1978).
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CC   pC194 is one of several R-plasmids found in S. aureus.
CC   pC194 belongs to incompatability group 8.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pC194)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Staphylococcus aureus)(B.subtilis BD170)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (R-plasmid incompatability group 8)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Staphylococcus
FT                   -> pC194 2910bp"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus R-plasmid incompatability group 8"
FT   misc_feature    complement(117..812)
FT                   /note="GEN Bacillus ORF A"
FT   misc_feature    708..1088
FT                   /note="GEN Bacillus ORF C (replication)"
FT   CDS             1260..1910
FT                   /note="ANT Bacillus chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (cmr/cml);
FT                   Bacillus gene B"
FT   misc_feature    complement(2424..2663)
FT                   /note="GEN Bacillus ORF D"
FT   misc_binding    0..0
FT                   /note="SIT unique HaeIII"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BglI"
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SQ   Sequence 2910 BP; 971 A; 486 C; 369 G; 1084 T; 0 other;
     gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta gcctctttaa
     ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat gcatcaaaga
     ctttttgatt tatttattaa ataatcacta tctttaccag aatacttagc catttcatat
     aattctttat tattattttg tcttattttt tgaacttgaa cttgtgttat ttctgaaatg
     cccgttacat cacgccataa atctaaccat tcttgttggc taatataata tcttttatct
     gtgaaatacg atttatttac tgcaattaac acatgaaaat gaggattata atcatctctt
     tttttattat atgtaatctc taacttacga acatatccct ttataacact acctactttt
     tttctcttta taagttttct aaaagaatta ttataacgtt ttatttcatt ttctaattca
     tcactcatta cattaggtgt agtcaaagtt aaaaagataa actccttttt ctcttgctgc
     ttaatatatt gcatcatcaa agataaaccc aatgcatctt ttctagcttt tctccaagca
     cagacaggac aaaatcgatt tttacaagaa ttagctttat ataatttctg tttttctaaa
     gttttatcag ctacaaaaga cagaaatgta ttgcaatctt caactaaatc catttgattc
     tctccaatat gacgtttaat aaatttctga aatacttgat ttctttgttt tttctcagta
     tacttttcca tgttataaca cataaaaaca acttagtttt cacaaactat gacaataaaa
     aaagttgctt tttccccttt ctatgtatgt tttttactag tcatttaaaa cgatacatta
     ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat aacttaaggg
     taactagcct cgccggcaat agttaccctt attatcaaga taagaaagaa aaggattttt
     cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg tggtctttat
     tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt
     aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag
     aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
     tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
     tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
     acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
     taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
     tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa
     gaaggttttt atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct
     ttttcgcttc tttattccaa ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc
     caaaacttgt cgaatggtcg gcttaatagc tcacgctatg ccgacattcg tctgcaagtt
     tagttaaggg ttcttctcaa cgcacaataa attttctcgg cataaatgcg tggtctaatt
     tttattttta ataaccttga tagcaaaaaa tgccattcca atacaaaacc acatacctat
     aatcgataac cacataacag tcataaaacc actccttttt aacaaacttt atcacaagaa
     atatttaaat tttaaatgcc tttattttga attttaaggg gcattttaaa gatttagggg
     taaatcatat agttttatgc ctaaaaacct acagaagctt ttaaaaagca aatatgagcc
     aaataaatat attctaattc tacaaacaaa aatttgagca aattcagtgt cgatttttta
     agacactgcc cagttacatg caaattaaaa ttttcatgat tttttatagt tcctaacagg
     gttaaaattt gtataacgaa agtataatgt ttatataacg ttagtataat aaagcatttt
     aacattatac ttttgataat cgtttatcgt cgtcatcaca ataactttta aaatactcgt
     gcataattca cgctgacctc ccaataacta catggtgtta tcgggaggtc agctgttagc
     acttatattt tgttattgtt cttcctcgat ttcgtctatc attttgtgat taatttctct
     tttttcttgt tctgttaagt cataaagttc actagctaaa tactcttttt gtttccaaat
     ataaaaaatt tgatagatat attacggttg
//