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ID   PCANTAB5   preliminary; circular DNA; SYN; 4472 BP.
XX
AC   IG1156;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCANTAB5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   M13KO7 from M13
RA   Vieira J., Messing J.;
RT   "Production of single-stranded plasmid DNA";
RL   Meth. Enzymol. 153:3-11(1987).
XX
CC   NM (pCANTAB5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pCANTAB5 4472bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4472 BP; 1103 A; 1064 C; 1129 G; 1176 T; 0 other;
     gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     ctgaagatca gttgggtgct cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     cgtgcataca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     accatgatta cgccaagctt tggagccttt tttttggaga ttttcaacgt gaaaaaatta
     ttattcgcaa ttcctttagt tgttcctttc tatgcggccc agccggccat ggcccaggtc
     caactgcagg agctcgagat caaacgggcg gccgcagaaa ctgttgaaag ttgtttagca
     aaacctcata cagaaaattc atttactaac gtctggaaag acgacaaaac tttagatcgt
     tacgctaact atgagggctg tctgtggaat gctacaggcg ttgtggtttg tactggtgac
     gaaactcagt gttacggtac atgggttcct attgggcttg ctatccctga aaatgagggt
     ggtggctctg agggtggcgg ttctgagggt ggcggttctg agggtggcgg tactaaacct
     cctgagtacg gtgatacacc tattccgggc tatacttata tcaaccctct cgacggcact
     tatccgcctg gtactgagca aaaccccgct aatcctaatc cttctcttga ggagtctcag
     cctcttaata ctttcatgtt tcagaataat aggttccgaa ataggcaggg tgcattaact
     gtttatacgg gcactgttac tcaaggcact gaccccgtta aaacttatta ccagtacact
     cctgtatcat caaaagccat gtatgacgct tactggaacg gtaaattcag agactgcgct
     ttccattctg gctttaatga ggatccattc gtttgtgaat atcaaggcca atcgtctgac
     ctgcctcaac ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct
     gagggtggcg gctctgaggg tggcggttct gagggtggcg gctctgaggg tggcggttcc
     ggtggcggct ccggttccgg tgattttgat tatgaaaaaa tggcaaacgc taataagggg
     gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat
     tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt
     gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc
     ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttctttgcct
     cagtcggttg aatgtcgccc ttatgtcttt ggcgctggta aaccatatga attttctatt
     gattgtgaca aaataaactt attccgtggt gtctttgcgt ttcttttata tgttgccacc
     tttatgtatg tattttcgac gtttgctaac atactgcgta ataaggagtc ttaataagaa
     ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     ccttacgcat ctgtgcggta tttcacaccg catataaatt gtaaacgtta atattttgtt
     aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg ttccagtttg
     gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg ggtcgaggtg
     ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     acagggcgcg tactatggtt gctttgacgg gtgcagtctc agtacaatct gctctgatgc
     cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     gaggttttca ccgtcatcac cgaaacgcgc ga
//