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ID   PCAT       preliminary; circular DNA; SYN; 3439 BP.
XX
AC   IG9866;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCAT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pBR328
RC   pGS8 from plasmid & vaccinia virus TK gene
RC   pMM1 from pUC9 & vaccinia virus TK gene
RC   pMM2 from pUC9 & vaccinia virus TK gene
RC   pMM3 from pMM1 & pMM2
RC   pMM4 from pMM3
RC   pMM5 from pMM4
RC   pGS15 from pUC9 & pAG4
RC   pGS19 from pGS15 & linker
RC   pGS20, pGS21 from pGS19 & pGS8
RC   [pGS30 from cat gene]
RC   pCAT from pBR328 & pUC7
RC   plasmid2 from pMM4 & pCAT
RC   plasmid3 from pGS20 & pCAT
RC   plasmid4 from pGS21 & pCAT
RC   vC1 from plasmid2 & vaccinia virus
RC   vC24 from plasmid2 & vaccinia virus
RC   vC30 from plasmid3 & vaccinia virus
RC   vC31 from plasmid3 & vaccinia virus
RA   Mackett M., Smith G.L., Moss B.;
RT   "General method for production and selection of infectious
RT   vaccinia virus recombinants expressing foreign genes";
RL   J. Virol. 49:857-864(1984).
XX
RN   [2]
RC   pCAT19 from pBR325 & pUC19
RA   Fuqua W.C.;
RT   "An improved chloramphenicol resistance gene cassette for
RT   site-directed marker replacement mutagenesis";
RL   Biotechniques 12:223-225(1992).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pCAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR328)(pUC7)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR328 TaqI-TaqI 773bp 3448..4221, cat gene
FT                   2. pUC7 remove AccI-AccI 12bp 247..259, MCS/2662bp
FT                   -> pCAT 3435bp"
FT   -               1..248
FT                   /note="pUC7 1..248 248bp
FT                   AccI = GT^MK AC
FT                   TaqI =     T^CGA"
FT   -               249..1023
FT                   /note="pBR328 3448..4222 775bp
FT                   TaqI =  T^CG A
FT                   AccI =    GT^MKAC"
FT   -               1024..3439
FT                   /note="pUC7 259..2674 2416bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-HindIII-HincII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3439 BP; 865 A; 841 C; 851 G; 882 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattccccgg
     atccgtcgcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
     cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     tcgcgacgga tccggggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     tgagcgagga agcggaaga
//