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ID   PCATCONTR  preliminary; circular DNA; SYN; 4750 BP.
XX
AC   X65321; IG0150;
XX
DT   11-APR-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCAT-Control - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCAT-Control, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [2]
RP   1-4750
RC   pCAT-Control
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
CC   pCAT-Control has promoter and enhancer. It has 4752 bp.
CC   NM (pCAT-Control)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli HB101)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (pBR322)
CC   BR (pCAT-Enhancer)(pCAT-Basic)(pCAT-Promoter)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp
FT                   2. oligo, MCS
FT                   3. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   4. SV40, small t antigen
FT                   -> pCAT-Control 4750bp"
FT   primer_bind     359..375
FT                   /note="PRI bacteriophage M13 forward"
FT   misc_binding    396..396
FT                   /note="SIT double EcoRI"
FT   misc_binding    403..403
FT                   /note="SIT unique BglII"
FT   promoter        408..610
FT                   /note="PRO SV40 genes"
FT   misc_feature    642..642
FT                   /note="ATG"
FT   CDS             642..1301
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    855..855
FT                   /note="SIT double EcoRI"
FT   CDS             1302..2242
FT                   /note="GEN SV40 small T antigen"
FT   misc_feature    1391..2241
FT                   /note="GEN SV40 small T antigen"
FT   promoter        2243..2479
FT                   /note="PRO SV40 enhancer"
FT   misc_feature    2438..2483
FT                   /note="MCS BamHI-XbaI-SalI-AccI-PstI-SphI"
FT   primer_bind     2531..2547
FT                   /note="PRI bacteriophage M13 reverse"
FT   CDS             complement(3690..4550)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4750 BP; 1241 A; 1103 C; 1133 G; 1273 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagatctgc atctcaatta
     gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     caaaaagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg
     aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag
     aaattcgccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     gtatcttatc atgtctggat ctgaaccatg gagcggagaa tgggcggaac tgggcggagt
     taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     taactgacac acattccaca gcagatcctc tagagtcgac ctgcaggcat gcaagcttgg
     cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta agagttcttg aagtggtggc ctaactacgg
     ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     aagagttggt agctcttgat ccggcaaaca accaccgctg gtagcggtgg tttttttgtt
     tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     ccctttcgtc
//