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ID   PCATENHAN  preliminary; circular DNA; SYN; 4610 BP.
XX
AC   X65319; IG0151;
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DT   11-APR-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCAT-Enhancer - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCAT-Enhancer, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-4610
RC   pCAT-Enhancer
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
CC   NM (pCAT-Enhancer)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli HB101)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (pBR322)
CC   BR (pCAT-Basic)(pCAT-Promoter)(pCAT-Control)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp
FT                   2. oligo, MCS
FT                   3. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   4. SV40, small t antigen
FT                   -> pCAT-Enhancer 4610bp"
FT   CDS             209..1069
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   primer_bind     2213..2229
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    2242..2271
FT                   /note="MCS HindIII-PstI-SalI-AccI-XbaI"
FT   misc_feature    2315..2315
FT                   /note="ATG"
FT   CDS             2315..2974
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    2528..2528
FT                   /note="SIT double EcoRI"
FT   CDS             3064..3914
FT                   /note="GEN SV40 small T antigen"
FT   promoter        3911..4156
FT                   /note="PRO SV40 enhancer"
FT   misc_binding    4166..4166
FT                   /note="SIT double EcoRI"
FT   primer_bind     4192..4208
FT                   /note="PRI bacteriophage M13 forward"
XX
SQ   Sequence 4610 BP; 1204 A; 1065 C; 1099 G; 1242 T; 0 other;
     ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     cagcgagtca gtgagcgagg aagcggaaga gcgccaatac gcaaaccgcc tctccccgcg
     cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     gagcgcaacg caattaatgt gagttacctc actcattagg caccccaggc tttacacttt
     atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag aggatctgag
     cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     atcatgtctg gatctgaacc atggagcgga gaatgggcgg aactgggcgg agttaggggc
     gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     cacacattcc acagcagatc ccccggaatt cactggccgt cgttttacaa cgtcgtgact
     gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatcccccc ttcgccagct
     ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tacactccgc
     tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
//