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ID   PCATPROMO  preliminary; circular DNA; SYN; 4506 BP.
XX
AC   X65320; IG0153;
XX
DT   11-APR-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCAT-Promoter - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCAT-Promoter, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [2]
RP   1-4506
RC   pCAT-Promoter
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
CC   NM (pCAT-Promoter)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli HB101)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (pBR322)
CC   BR (pCAT-Enhancer)(pCAT-Basic)(pCAT-Control)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp
FT                   2. oligo, MCS
FT                   3. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   4. SV40, small t antigen
FT                   -> pCAT-Promoter 4506bp"
FT   primer_bind     359..375
FT                   /note="PRI bacteriophage M13 forward"
FT   misc_binding    396..396
FT                   /note="SIT double EcoRI"
FT   misc_binding    403..403
FT                   /note="SIT unique BglII"
FT   promoter        408..610
FT                   /note="PRO SV40 promoter"
FT   misc_feature    642..642
FT                   /note="ATG"
FT   CDS             642..1301
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    855..855
FT                   /note="SIT double EcoRI"
FT   CDS             1302..2236
FT                   /note="GEN SV40 small T antigen"
FT   misc_feature    1391..2241
FT                   /note="GEN SV40 small T antigen"
FT   misc_binding    2237..2265
FT                   /note="MCS BamHI-XbaI-SalI-AccI-PstI-SphI"
FT   misc_binding    2243..2267
FT                   /note="MCS (Hind III-Xba I)"
FT   primer_bind     2285..2301
FT                   /note="PRI bacteriophage M13 reverse"
FT   CDS             complement(3446..4306)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4506 BP; 1192 A; 1048 C; 1052 G; 1214 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagatctgc atctcaatta
     gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     caaaaagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg
     aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag
     aaattcgccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     gtatcttatc atgtctggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat
     catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtc
//