back Return to this vector's summary.
ID   PCDM8      preliminary; circular DNA; SYN; 4356 BP.
XX
AC   IG0331; M59925; M16445; X06296;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pCDM8 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   from pCDM8
RA   Bernot A., Auffray C.;
RT   "Primary structure and ontogeny of an avian CD3 transcript";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:2550-2554(1991).
XX
RN   [2]
RC   pcDNA-IIb from pcDNA1 & synapsin IIb
RC   from pMAMneo
RA   Han H.Q., Nichols R.A., Rubin M.R., Bahler M., Greengard P.;
RT   "Induction of formation of presynaptic terminals in neuroblastoma
RT   cells by synapsin IIb";
RL   Nature 349:697-700(1991).
XX
RN   [3]
RC   pT22 from human H-ras
RC   pIA from adenovirus E1A
RA   Prendergast G.C., Ziff E.B.;
RT   "Methylation-sensitive sequence-specific DNA binding by the c-Myc
RT   basic region";
RL   Science 251:186-189(1991).
XX
RN   [4]
RC   [pCDM8 from pBR322 & polyoma & CMV & SV40 & M13]
RC   [pcDNAI or pcDNA1 from pCDM8]
RC   from pCDM8
RA   Seed B.;
RT   "An LFA-3 cDNA encodes a phospholipid-linked membrane protein
RT   homologous to its receptor CD2";
RL   Nature 329:840-842(1987).
XX
RN   [5]
RC   from pcDNA1
RA   Thomsen G., Woolf T., Whitman M., Sokol S., Vaughan J., Vale W.,
RA   Melton D.A.;
RT   "Activins are expressed early in Xenopus embryogenesis and can
RT   induce axial mesoderm and anterior structures";
RL   Cell 63:485-493(1990).
XX
CC   NM (pCDM8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli MC1061P3)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (pcDNAI)(pcDNAIAmp)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. piVX, ori
FT                   2. M13, ori
FT                   3. supF gene
FT                   4. CMV, promoter
FT                   5. SV40, splice/polyA
FT                   6. polyoma, ori
FT                   7. SV40, ori
FT                   -> pCDM8 4356bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    587..587
FT                   /note="SIT NheI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13"
FT   misc_binding    1179..1179
FT                   /note="SIT SacII"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli supF gene"
FT   promoter        0..0
FT                   /note="PRO human cytomegalovirus (CMV)
FT                   immediate early gene"
FT   misc_binding    1790..1790
FT                   /note="SIT NdeI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-XbaI-XhoI-BstXI...-
FT                   BstXI-XhoI-PstI-NotI-XbaI"
FT   repeat_unit     0..0
FT                   /note="RPT stuffer (358 bp)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    3220..3220
FT                   /note="SIT HpaI"
FT   misc_binding    3353..3353
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI polyoma virus"
FT   misc_binding    3742..3742
FT                   /note="SIT BclI"
FT   misc_binding    4024..4024
FT                   /note="SIT ApaI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   terminator      0..0
FT                   /note="TER SV40 early gene"
FT   misc_binding    3955..3955
FT                   /note="SIT SfiI"
XX
SQ   Sequence 4356 BP; 1149 A; 1056 C; 1066 G; 1085 T; 0 other;
     ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     acggggggtt cgtgcacaga gcccagcttg gagcgaacga cctacaccga actgagatac
     ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     tgctatcttt atgatcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
     agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
     gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
     acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     tatcgaaatt aatacgactc actataggga gacccaagct tctagagatc cctcgacctc
     gagatccatt gtgctggcgc ggattcttta tcactgataa gttggtggac atattatgtt
     tatcagtgat aaagtgtcaa gcatgacaaa gttgcagccg aatacagtga tccgtgccgg
     ccctggactg ttgaacgagg tcggcgtaga cggtctgacg acacgcaaac tggcggaacg
     gttgggggtg cagcagccgg cgctttactg gcacttcagg aacaagcggg cgctgctcga
     cgcactggcc gaagccatgc tggcggagaa tcatacgctt cggtgccgag agccgacgac
     gactggcgct catttctgat cgggaatccc gcagcttcag gcaggcgctg ctcgcctacc
     gccagcacaa tggatctcga gggatcttcc atacctacca gttctgcgcc tgcaggtcgc
     ggccgcgact ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     gtgttaaact actgattcta attgttgtgg tattttagat tccaacctat ggaacttatg
     aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     gaaaggtaga agaccccaag gactttcctt cagaattggt aagttttttg agtcatgctg
     tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata
     atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttatcactg
     cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcccgcca
     tggtatcaac gccatatttc tatttacagt agggacctct tcgttgtgta ggtaccgctg
     tattcctagg gaaatagtag aggcaccttg aactgtctgc atcagccata tagcccccgc
     tgttcgactt acaaacacag gcacagtact gacaaaccca tacacctcct ctgaaatacc
     catagttgct agggctgtct ccgaactcat tacaccctac caagtgagag ctgtaatttc
     gcgatcaagg gcagcgaggg cttctccaga taaaatagct tctgccgaga gtcccgtaag
     ggtagacact tcagctaatc cctcgatgag gtctactaga atagtcagtg cggctcccat
     tttgaaaatt cacttacttg atcagcttca gaagatgggc gagggcctcc aacacagtaa
     ttttcctccc gactcttaaa atagaaaatg tcaagtcagt taaggaggaa gtggactaac
     tgacgcagct ggccgtgcga catcctcttt taattagttg ctaggcaacg ccctccagag
     ggcgtgtggt tttgcaagag gaagcaaaag cctctccacc caggcctaga atgtttccac
     ccaatcatta ctatgacaac agctgttttt tttagtatta agcagaggcc ggggacccct
     gggcccgctt actctggaga aaaagaagag aggcattgta gaggcttcca gaggcaactt
     gtcaaaacag gactgcttct atttctgtca cactgtctgg ccctgtcaca aggtccagca
     cctccatacc ccctttaata agcagtttgg gaacgggtgc gggtcttact ccgcccatcc
     cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     tttttggagg cctaggcttt tgcaaaaagc taattc
//