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ID   PCDNA3     preliminary; circular DNA; SYN; 5446 BP.
XX
AC   IG1046;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pcDNA3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCA from pRIT2T & pcDNAII
RC   pCA-SEB from pCA & pSK401
RA   Buelow R., O'Hehir R.E., Schreifels R., Kummerehl T.J., Riley G.,
RA   Lamb J.R.;
RT   "Localization of the immunologic activity in the superantigen
RT   Staphylococcal enterotoxin B using truncated recombinant fusion
RT   proteins";
RL   J. Immunol. 148:1-6(1992).
XX
RN   [2]
RC   [pcDNAII from pcDNAI]
RC   from pcDNAII
RA   Rangnekar V.V., Waheed S., Davies T.J., Toback F.G., Rangnekar V.M.;
RT   "Antimitogenic and mitogenic actions of interleukin-1 in diverse
RT   cell types are associated with induction of gro gene expression";
RL   J. Biol. Chem. 266:2415-2422(1991).
XX
CC   NM (pcDNA3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning cDNA)(deletions nested)
CC   SE ()
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pcDNAII 3013bp, M13 ori/amp gene
FT                   -> pcDNA3 5446bp"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS ApaI-XbaI-NsiI-SphI-XhoI-XmaIII-NotI-
FT                   BstXI-EcoRV-PstI-EcoRI-BstXI-XmaIII-SpeI-BamHI-
FT                   SacI-KpnI-HindIII-NsiI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    511..511
FT                   /note="SIT AflIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1910..1910
FT                   /note="SIT SacII"
FT   misc_binding    1999..1999
FT                   /note="SIT XmnI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 5446 BP; 1255 A; 1417 C; 1390 G; 1384 T; 0 other;
     gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
     gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
     ctggcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
     gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
     ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
     gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//