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ID   PCDNAI     preliminary; circular DNA; SYN; 4033 BP.
XX
AC   IG1047; M59925; M16445; X06296;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pcDNAI - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pCDM8 from pBR322 & polyoma & CMV & SV40 & M13]
RC   [pcDNAI or pcDNA1 from pCDM8]
RC   from pCDM8
RA   Seed B.;
RT   "An LFA-3 cDNA encodes a phospholipid-linked membrane protein
RT   homologous to its receptor CD2";
RL   Nature 329:840-842(1987).
XX
CC   NM (pcDNAI)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli MC1061P3)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pCDM8)
CC   BR (pcDNAIAmp)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCDM8 4356bp
FT                   -> pcDNAI 4033bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    587..587
FT                   /note="SIT NheI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13"
FT   misc_binding    1179..1179
FT                   /note="SIT SacII"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli supF gene"
FT   promoter        0..0
FT                   /note="PRO human cytomegalovirus (CMV)
FT                   immediate early gene"
FT   misc_binding    1790..1790
FT                   /note="SIT NdeI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BamHI-BstXI-EcoRI-PstI-EcoRV-
FT                   BstXI-NotI-XhoI-SphI-NsiI-XbaI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    2892..2892
FT                   /note="SIT HpaI"
FT   rep_origin      0..0
FT                   /note="ORI polyoma virus"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   terminator      0..0
FT                   /note="TER SV40 early gene"
FT   misc_binding    3955..3955
FT                   /note="SIT SfiI"
XX
SQ   Sequence 4033 BP; 1082 A; 971 C; 959 G; 1021 T; 0 other;
     ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
     agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
     gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
     acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag ctcggatcca
     ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc
     gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag gatctttgtg
     aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     catcaatgta tcttatcatg tctggatcat cccgccatgg tatcaacgcc atatttctat
     ttacagtagg gacctcttcg ttgtgtaggt accgctgtat tcctagggaa atagtagagg
     caccttgaac tgtctgcatc agccatatag cccccgctgt tcgacttaca aacacaggca
     cagtactgac aaacccatac acctcctctg aaatacccat agttgctagg gctgtctccg
     aactcattac accctccaaa gtcagagctg taatttcgcc atcaagggca gcgagggctt
     ctccagataa aatagcttct gccgagagtc ccgtaagggt agacacttca gctaatccct
     cgatgaggtc tactagaata gtcagtgcgg ctcccatttt gaaaattcac ttacttgatc
     agcttcagaa gatggcggag ggcctccaac acagtaattt tcctcccgac tcttaaaata
     gaaaatgtca agtcagttaa gcaggaagtg gactaactga cgcagctggc cgtgcgacat
     cctcttttaa ttagttgcta ggcaacgccc tccagagggc gtgtggtttt gcaagaggaa
     gcaaaagcct ctccacccag gcctagaatg tttccaccca atcattacta tgacaacagc
     tgtttttttt agtattaagc agaggccggg gacccctggg cccgcttact ctggagaaaa
     agaagagagg cattgtagag gcttccagag gcaacttgtc aaaacaggac tgcttctatt
     tctgtcacac tgtctggccc tgtcacaagg tccagcacct ccataccccc tttaataagc
     agtttgggaa cgggtgcggg tcttactccg cccatcccgc ccctaactcc gcccagttcc
     gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     aaaaagctaa ttc
//