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ID   PCDNAIAMP  preliminary; circular DNA; SYN; 4801 BP.
XX
AC   IG1048; M59925; M16445; X06296;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pcDNAIAmp - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pCDM8 from pBR322 & polyoma & CMV & SV40 & M13]
RC   [pcDNAI or pcDNA1 from pCDM8]
RC   from pCDM8
RA   Seed B.;
RT   "An LFA-3 cDNA encodes a phospholipid-linked membrane protein
RT   homologous to its receptor CD2";
RL   Nature 329:840-842(1987).
XX
CC   NM (pcDNAIAmp)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pCDM8)
CC   BR (pcDNAI)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pcDNAI 4033bp
FT                   2. pBR322 800bp, amp gene
FT                   -> pcDNAIAmp 4801bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    587..587
FT                   /note="SIT NheI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1794..1794
FT                   /note="SIT PvuI"
FT   promoter        0..0
FT                   /note="PRO human cytomegalovirus (CMV)
FT                   immediate early gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BamHI-BstXI-EcoRI-EcoRV-
FT                   BstXI-NotI-XhoI-SphI-NsiI-XbaI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    3816..3816
FT                   /note="SIT HpaI"
FT   rep_origin      0..0
FT                   /note="ORI polyoma virus"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   terminator      0..0
FT                   /note="TER SV40 early gene"
FT   misc_binding    4880..4880
FT                   /note="SIT SfiI"
XX
SQ   Sequence 4801 BP; 1300 A; 1126 C; 1133 G; 1242 T; 0 other;
     ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
     agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
     gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
     acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     aagggatttt agacaggaac ggtacgccag ctggattacc aaagggcctc gtgatacgcc
     tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     aacttcattt ttaatttctc tagcgcgttg acattgatta ttgactagtt attaatagta
     atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
     acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc
     gccagtgtgc tggaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct
     agagggccct attctatagt gtcacctaaa tgctagagga tctttgtgaa ggaaccttac
     ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat
     agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     ttatcatgtc tggatcatcc cgccatggta tcaacgccat atttctattt acagtaggga
     cctcttcgtt gtgtaggtac cgctgtattc ctagggaaat agtagaggca ccttgaactg
     tctgcatcag ccatatagcc cccgctgttc gacttacaaa cacaggcaca gtactgacaa
     acccatacac ctcctctgaa atacccatag ttgctagggc tgtctccgaa ctcattacac
     cctccaaagt cagagctgta atttcgccat caagggcagc gagggcttct ccagataaaa
     tagcttctgc cgagagtccc gtaagggtag acacttcagc taatccctcg atgaggtcta
     ctagaatagt cagtgcggct cccattttga aaattcactt acttgatcag cttcagaaga
     tggcggaggg cctccaacac agtaattttc ctcccgactc ttaaaataga aaatgtcaag
     tcagttaagc aggaagtgga ctaactgacg cagctggccg tgcgacatcc tcttttaatt
     agttgctagg caacgccctc cagagggcgt gtggttttgc aagaggaagc aaaagcctct
     ccacccaggc ctagaatgtt tccacccaat cattactatg acaacagctg ttttttttag
     tattaagcag aggccgggga cccctgggcc cgcttactct ggagaaaaag aagagaggca
     ttgtagaggc ttccagaggc aacttgtcaa aacaggactg cttctatttc tgtcacactg
     tctggccctg tcacaaggtc cagcacctcc ataccccctt taataagcag tttgggaacg
     ggtgcgggtc ttactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaatt
     c
//