back Return to this vector's summary.
ID   PCDNAII    preliminary; circular DNA; SYN; 3013 BP.
XX
AC   IG1049;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pcDNAII - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCA from pRIT2T & pcDNAII
RC   pCA-SEB from pCA & pSK401
RA   Buelow R., O'Hehir R.E., Schreifels R., Kummerehl T.J., Riley G.,
RA   Lamb J.R.;
RT   "Localization of the immunologic activity in the superantigen
RT   Staphylococcal enterotoxin B using truncated recombinant fusion
RT   proteins";
RL   J. Immunol. 148:1-6(1992).
XX
RN   [2]
RC   [pcDNAII from pcDNAI]
RC   from pcDNAII
RA   Rangnekar V.V., Waheed S., Davies T.J., Toback F.G., Rangnekar V.M.;
RT   "Antimitogenic and mitogenic actions of interleukin-1 in diverse
RT   cell types are associated with induction of gro gene expression";
RL   J. Biol. Chem. 266:2415-2422(1991).
XX
RN   [3]
RC   pSK401 from Staphylococcus enterotoxin B gene
RA   Ranelli D.M., Jones C.L., Johns M.B., Mussey G.J., Khan S.A.;
RT   "Molecular cloning of staphylococcal enterotoxin B gene in
RT   Escherichia coli and Staphylococcus aureus";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:5850-5854(1985).
XX
CC   NM (pcDNAII)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning cDNA)(deletions nested)
CC   SE ()
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pcDNAI, M13 ori/amp gene/4033bp-
FT                   -> pcDNAII 3013bp"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS ApaI-XbaI-NsiI-SphI-XhoI-XmaIII-NotI-
FT                   BstXI-EcoRV-PstI-EcoRI-BstXI-XmaIII-SpeI-BamHI-
FT                   SacI-KpnI-HindIII-NsiI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    511..511
FT                   /note="SIT AflIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1910..1910
FT                   /note="SIT SacII"
FT   misc_binding    1999..1999
FT                   /note="SIT XmnI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 3013 BP; 776 A; 738 C; 759 G; 740 T; 0 other;
     gggcgaattg ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
     gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca agcttgatgc
     atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
     ttcctgtgtg aaattgttat tcgctcacaa ttccacacaa catacgagcc ggaagcataa
     agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaagccca
     ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagcgcttac
     catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     cctccattca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     gtttgcgcaa cgttgttggc attgctacag gcatcgtggt gtcactctcg tcgtttggta
     tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     gaccgagttg ctcttgcccg gcgtcaatac gggataatag tgtatcacat agcagaactt
     taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgtatgcggt gtgaaatacc
     gcacagatgc gtaaggagaa aataccgcat caggcgaaat tgtaaacgtt aatattttgt
     taaaattcgc gttaaatatt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     gcaaaatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt gttccagttt
     ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg cggtcgaggt
     gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     acgactcact ata
//