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ID PCDNAINEO preliminary; circular DNA; SYN; 6969 BP.
XX
AC IG1050;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pcDNAINeo - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pGEM1-SKR from pGEM-1 & SKR gene
RC pcDNA1-SKR from pcDNA1 & pGEM1-SKR
RC from pRc/CMV & pcDNA1-SKR, SKR gene
RC [pcDNAINeo from pcDNAI & neo gene]
RA DeBernardi M.A., Seki T., Brooker G.;
RT "Inhibition of cAMP accumulation by intracellular calcium
RT mobilization in C6-2B cells stably transfected with substance K
RT receptor cDNA";
RL Proc. Natl. Acad. Sci. U.S.A. 88:9257-9261(1991).
XX
RN [2]
RC from pcDNAINeo
RA Seykora J.T., Ravetch J.V., Aderem A.;
RT "Cloning and molecular characterization of the murine macrophage
RT 68-kDa protein kinase C substrate and its regulation by
RT bacterial lipopolysaccharide";
RL Proc. Natl. Acad. Sci. U.S.A. 88:2505-2509(1991).
XX
RN [3]
RC pcDNAINeo-PSF from pcDNAINeo & PSF gene
RC RSV.5gpt-PSF from RSV.5(gpt) & PSF gene
RA Spies T., DeMars R.;
RT "Restored expression of major histocompatibility class I molecules
RT by gene transfer of a putative peptide transporter [see comments]";
RL Nature 351:323-324(1991).
XX
CC NM (pcDNAINeo)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (E.coli MC1061P3)(mammal)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pcDNAI)
CC BR (pcDNAIAmp)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pcDNAI 4033bp
FT 2. Tn5 BglII-BglII 2784bp 1516..4300, neo gene
FT -> pcDNAINeo 6969bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage M13"
FT misc_binding 1179..1179
FT /note="SIT SacII"
FT CDS complement(0..0)
FT /note="GEN E. coli supF gene"
FT promoter 0..0
FT /note="PRO human cytomegalovirus (CMV)
FT immediate early gene"
FT misc_binding 1790..1790
FT /note="SIT NdeI"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="MCS HindIII-BamHI-BstXI-EcoRV-
FT BstXI-NotI-XhoI-NsiI-XbaI"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT rep_origin 0..0
FT /note="ORI SV40"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT promoter 0..0
FT /note="PRO Rous sarcoma virus (RSV) long terminal
FT repeat (LTR)"
FT misc_binding 3470..3470
FT /note="SIT PvuI"
FT misc_binding 3939..3939
FT /note="SIT BglII"
FT CDS 0..0
FT /note="ANT E. coli neomycin phosphotransferase II gene
FT (NPTII), neomycin resistance gene (neo)"
FT misc_binding 4940..4940
FT /note="SIT XmaI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 5962..5962
FT /note="SIT ClaI"
FT rep_origin 0..0
FT /note="ORI polyoma virus"
FT misc_feature 0..0
FT /note="SPL SV40 splicing sequences"
FT terminator 0..0
FT /note="TER SV40 early gene"
XX
SQ Sequence 6969 BP; 1809 A; 1656 C; 1739 G; 1765 T; 0 other;
ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag ctcggatcca
ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc
gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag gatctttgtg
aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
catcaatgta tcttatcatg tctggatctc ccgatcccct atggtgcact ctcagtacaa
tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg
ttaggagtcc cctcaggata tagtagtttc gcttttgcat agggaggggg aaatgtagtc
ttatgcaata cacttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
attaggaagg caacagacag gtctgacatg gattggacga accactgaat tccgcattgc
agagataatt gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca
cattggtgtg cacctcctaa gctagctttg ccgcaagcac tcagggcgca agggctgcta
aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg
aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc
agcagcggct atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc
gctttggtcc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
atgtatctta tcatgtctgg atcgatcccg ccatggtatc aacgccatat ttctatttac
agtagggacc tcttcgttgt gtaggtaccg ctgtattcct agggaaatag tagaggcacc
ttgaactgtc tgcatcagcc atatagcccc cgctgttcga cttacaaaca caggcacagt
actgacaaac ccatacacct cctctgaaat acccatagtt gctagggctg tctccgaact
cattacaccc tccaaagtca gagctgtaat ttcgccatca agggcagcga gggcttctcc
agataaaata gcttctgccg agagtcccgt aagggtagac acttcagcta atccctcgat
gaggtctact agaatagtca gtgcggctcc cattttgaaa attcacttac ttgatcagct
tcagaagatg gcggagggcc tccaacacag taattttcct cccgactctt aaaatagaaa
atgtcaagtc agttaagcag gaagtggact aactgacgca gctggccgtg cgacatcctc
ttttaattag ttgctaggca acgccctcca gagggcgtgt ggttttgcaa gaggaagcaa
aagcctctcc acccaggcct agaatgtttc cacccaatca ttactatgac aacagctgtt
ttttttagta ttaagcagag gccggggacc cctgggcccg cttactctgg agaaaaagaa
gagaggcatt gtagaggctt ccagaggcaa cttgtcaaaa caggactgct tctatttctg
tcacactgtc tggccctgtc acaaggtcca gcacctccat acccccttta ataagcagtt
tgggaacggg tgcgggtctt actccgccca tcccgcccct aactccgccc agttccgccc
attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
agctaattc
//