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ID   PCDNAINEO  preliminary; circular DNA; SYN; 6969 BP.
XX
AC   IG1050;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pcDNAINeo - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGEM1-SKR from pGEM-1 & SKR gene
RC   pcDNA1-SKR from pcDNA1 & pGEM1-SKR
RC   from pRc/CMV & pcDNA1-SKR, SKR gene
RC   [pcDNAINeo from pcDNAI & neo gene]
RA   DeBernardi M.A., Seki T., Brooker G.;
RT   "Inhibition of cAMP accumulation by intracellular calcium
RT   mobilization in C6-2B cells stably transfected with substance K
RT   receptor cDNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:9257-9261(1991).
XX
RN   [2]
RC   from pcDNAINeo
RA   Seykora J.T., Ravetch J.V., Aderem A.;
RT   "Cloning and molecular characterization of the murine macrophage
RT   68-kDa protein kinase C substrate and its regulation by
RT   bacterial lipopolysaccharide";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:2505-2509(1991).
XX
RN   [3]
RC   pcDNAINeo-PSF from pcDNAINeo & PSF gene
RC   RSV.5gpt-PSF from RSV.5(gpt) & PSF gene
RA   Spies T., DeMars R.;
RT   "Restored expression of major histocompatibility class I molecules
RT   by gene transfer of a putative peptide transporter [see comments]";
RL   Nature 351:323-324(1991).
XX
CC   NM (pcDNAINeo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli MC1061P3)(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pcDNAI)
CC   BR (pcDNAIAmp)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pcDNAI 4033bp
FT                   2. Tn5 BglII-BglII 2784bp 1516..4300, neo gene
FT                   -> pcDNAINeo 6969bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13"
FT   misc_binding    1179..1179
FT                   /note="SIT SacII"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli supF gene"
FT   promoter        0..0
FT                   /note="PRO human cytomegalovirus (CMV)
FT                   immediate early gene"
FT   misc_binding    1790..1790
FT                   /note="SIT NdeI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BamHI-BstXI-EcoRV-
FT                   BstXI-NotI-XhoI-NsiI-XbaI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    3470..3470
FT                   /note="SIT PvuI"
FT   misc_binding    3939..3939
FT                   /note="SIT BglII"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   misc_binding    4940..4940
FT                   /note="SIT XmaI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    5962..5962
FT                   /note="SIT ClaI"
FT   rep_origin      0..0
FT                   /note="ORI polyoma virus"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   terminator      0..0
FT                   /note="TER SV40 early gene"
XX
SQ   Sequence 6969 BP; 1809 A; 1656 C; 1739 G; 1765 T; 0 other;
     ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
     agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
     gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
     acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag ctcggatcca
     ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc
     gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag gatctttgtg
     aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     catcaatgta tcttatcatg tctggatctc ccgatcccct atggtgcact ctcagtacaa
     tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg
     ttaggagtcc cctcaggata tagtagtttc gcttttgcat agggaggggg aaatgtagtc
     ttatgcaata cacttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     attaggaagg caacagacag gtctgacatg gattggacga accactgaat tccgcattgc
     agagataatt gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca
     cattggtgtg cacctcctaa gctagctttg ccgcaagcac tcagggcgca agggctgcta
     aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg
     aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc
     agcagcggct atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc
     gctttggtcc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     atgtatctta tcatgtctgg atcgatcccg ccatggtatc aacgccatat ttctatttac
     agtagggacc tcttcgttgt gtaggtaccg ctgtattcct agggaaatag tagaggcacc
     ttgaactgtc tgcatcagcc atatagcccc cgctgttcga cttacaaaca caggcacagt
     actgacaaac ccatacacct cctctgaaat acccatagtt gctagggctg tctccgaact
     cattacaccc tccaaagtca gagctgtaat ttcgccatca agggcagcga gggcttctcc
     agataaaata gcttctgccg agagtcccgt aagggtagac acttcagcta atccctcgat
     gaggtctact agaatagtca gtgcggctcc cattttgaaa attcacttac ttgatcagct
     tcagaagatg gcggagggcc tccaacacag taattttcct cccgactctt aaaatagaaa
     atgtcaagtc agttaagcag gaagtggact aactgacgca gctggccgtg cgacatcctc
     ttttaattag ttgctaggca acgccctcca gagggcgtgt ggttttgcaa gaggaagcaa
     aagcctctcc acccaggcct agaatgtttc cacccaatca ttactatgac aacagctgtt
     ttttttagta ttaagcagag gccggggacc cctgggcccg cttactctgg agaaaaagaa
     gagaggcatt gtagaggctt ccagaggcaa cttgtcaaaa caggactgct tctatttctg
     tcacactgtc tggccctgtc acaaggtcca gcacctccat acccccttta ataagcagtt
     tgggaacggg tgcgggtctt actccgccca tcccgcccct aactccgccc agttccgccc
     attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     agctaattc
//