back Return to this vector's summary.
ID   PCDV1      preliminary; circular DNA; SYN; 3082 BP.
XX
AC   IG1051; ATCC37368;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pcDV1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR322SV from pBR322 & SV40 late polyA
RC   pcDV1 from pBR322SV
RC   pBR322-SV40 from pBR322 & SV40 early prom/splice acceptor/ori
RC   pL1 from pBR322-SV40
RC   [pcD-X from pcDV1]
RC   pcD-dhfr from pcDV1 & pL1 & DHFR gene
RC   pcD-alphaG from pcDV1 & pL1 & alpha-globin gene
RA   Okayama H., Berg P.;
RT   "A cDNA cloning vector that permits expression of cDNA inserts
RT   in mammalian cells";
RL   Mol. Cell. Biol. 3:280-289(1983).
XX
RN   [2]
RC   pBR322SV from pBR322 & SV40 late polyA
RC   pcDV1 from pBR322SV
RC   pBR322-SV40 from pBR322 & SV40 early prom/splice acceptor/ori
RC   pL1 from pBR322-SV40
RA   Okayama H., Berg P.;
RT   "High efficiency cloning of full-length cDNA";
RL   Mol. Cell. Biol. 2:161-170(1982).
XX
RN   [3]
RC   from pbetaG1
RA   Efstratiadis A., Kafatos F.C., Maniatis T.;
RT   "The primary structure of rabbit beta-globin mRNA as determined
RT   from cloned DNA";
RL   Cell 10:571-585(1977).
XX
RN   [4]
RC   pBR322-SV40 from pBR322 & SV40
RA   Subramani S.;
RT   ;
RL   Unpublished (1982).
XX
RN   [5]
RC   pbetaG1 or pMB9 betaGLOBIN 1 from pMB9 & rabbit beta-globin gene
RC   pbetaG or pMB9 betaGLOBIN from pMB9 & rabbit beta-globin gene
RA   Maniatis T., Sim G.K., Efstratiadis A., Kafatos F.C.;
RT   "Amplification and characterization of a beta-globin gene
RT   synthesized in vitro";
RL   Cell 8:163-182(1976).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   pcDV1 is an oligo dT-tailed plasmid primer. It is 3088 bp untailed.
CC   It works together with oligo dG-tailed pL1 linker, which has
CC   SV40 origin, SV40 early promoter, and SV40 mRNA splicing, and has
CC   a HindIII at one end and polyG at the PstI site at the other.
CC   pL1 linker has a BglI site in SV40 ori and an XhoI and BamHI site.
CC   A 16S splice junction is at the BAmHI site.
CC   DNA ligase, RNaseH, and DNA polymerase I makes a dsDNA circle.
CC   cDNA synthesis is from polyA mRNA and is oriented,
CC   with linker 5' and polyA signal on plasmid 3' to cDNA.
CC   The SV40 segment contains KpnI and EcoRI sites for the preparation
CC   of vector-primer containing a poly(dT) tail at one end & the
CC   polyadenylation sequence from the late region of the virus.
CC   pcDV1 (ATCC 37368) and pL1 (ATCC 37369) should be used together to
CC   construct a cDNA library.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pcDV1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)(ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove HindIII-PvuII 2037bp 30..2067,
FT                   \ tet/2324bp
FT                   2. SV40 PvuII-HindIII 774bp 273..1047
FT                   \ late polyA/0.71 to 0.86 3722..4509 [787bp]
FT                   -> pBR322-SV40 3102bp
FT                   1. pBR322-SV40 remove HpaI-HpaI 20bp 502..522, 3082bp
FT                   \ SV40 HpaI 502 522 2669 3737 or SV40 KpnI 299
FT                   oligo dT tail:oligo dT tail
FT                   -> pcDV1 3088bp"
FT   -               1..2324
FT                   /note="pBR322 2067..4361..29 2324bp
FT                   HindIII = A^AGCTT"
FT   -               2325..2853
FT                   /note="SV40 522..1050 529bp complement
FT                   HpaI = GTT^AAC"
FT   -               2854..3082
FT                   /note="SV40 273..501 229bp complement
FT                   PvuII = CAG^CTG"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
XX
SQ   Sequence 3082 BP; 833 A; 771 C; 728 G; 750 T; 0 other;
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     tttcgtcttc aagaattctc atgtttgaca gcttatcatc gataagcttg agaaatggca
     ttaaaaagtg ttggacccca atgtctgggg tcaagatact gaacactgtg aacaaaggtt
     tgtactccag gaaataaaat atcatagtaa tcatctggcc tatacaaatc tacagccatt
     cctggttgtt gatataaacc aacagtagaa actttgtgat cccagtcact aaaaaatcta
     taccccactt gagcaacagc gctcacacca gtcacagttt gcagtaaagc tgcaaatcca
     gctatagcag caggagcccc agatatcaca gcataggcct gtggagtgag gcctatagca
     gcaattgcct cagaggttgt taggccttca acagtagcaa cagatgcaag ttgcacttca
     attgcagcag cggcctctcc agcagcaatt tcagctactg aaaatccagt agcagcagca
     gcttcagaca cagtagcaat taggtccccc aacagtgtta aagcagcacc catggacctg
     aaataaaaga caaaaagact aaacttacca gttaaccttt ctggtttttt gcgtttcccg
     tcaacagtat cttccccttc acaaaattgc agcaaaagct ctaaaacaaa cacaaaaagg
     cgttgagctg tttttttact ttcagtccat gacctacgaa ccttaacgga ggcctggcgt
     gacagccggc gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac
     cttctgaggc ggaaagaacc ag
//