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ID   PCDX       preliminary; circular DNA; SYN; 3053 BP.
XX
AC   IG0031;
XX
DT   11-DEC-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mammal/E. coli plasmid vector pcD-X (with no X) - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR322SV from pBR322 & SV40 late polyA
RC   pcDV1 from pBR322SV or pSV1
RC   pL1 from pBR322 & SV40 early promoter/splice/ori & linker
RC   [pcD-X from pcDV1]
RC   pcD-dhfr from pcDV1 & pL1 & dhfr
RC   pcD-alphaG from pcDV1 & pL1 & alpha-globin gene
RA   Okayama H., Berg P.;
RT   "A cDNA cloning vector that permits expression of cDNA inserts
RT   in mammalian cells";
RL   Mol. Cell. Biol. 3:280-289(1983).
XX
CC   NM (pcD-X)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mammal)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pcDV1)(pL1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pcDV1
FT                   tailing
FT                   2. X gene
FT                   tailing
FT                   3. pL1
FT                   tailing
FT                   -> pcD-X 3053bp [X is the gene]"
FT   rep_origin      751..751
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1509..2369)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      2853..2928
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
XX
SQ   Sequence 3053 BP; 779 A; 755 C; 740 G; 779 T; 0 other;
     gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     cctctacaaa tgtggtatgg ctgattatga tcctgcctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgccgcga ccgagttgct
     cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactactc ttcctttttc aatattattg aagcatttat cagggttatt
     gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     cctataaaaa taggcgtatc acgaggccct ttcgtcttca aggtcgacct catgtttgac
     agcttatcat cgataagctt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     agctcctcga ggaactgaaa aaccagaaag ttaactggta agtttagtct ttttgtcttt
     tatttcaggt cccggatccg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc
     tttacttcta ggcctgtacg gaagtgttac ttctgctcta aaagctgctg cag
//