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ID   PCEP4      preliminary; circular DNA; SYN; 10410 BP.
XX
AC   IG1052; K01383;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCEP4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pcDV1/S2 from pcDV1 & linker
RC   pRSVCAT/H2 from pRSVcat & linker
RC   [pBT from BT gene]
RC   pRSV/BT from pRSVCAT/H2 & pBT
RC   pRSVPA1 from pRSV/BT & pcDV1/S2
RC   pRSVPA2 from pRSVPA1 & linker
RC   p220.2 from p201
RC   p220.2/B- from p220.2
RC   pREP2 from pRSVPA2 & p220.2/B-
RC   pREP3 from pREP2
RC   p220.2/XS from p220.2/B-
RC   pHS1PA4 from pRSVPA2
RC   pMEP4 from p220.2/XS & pHS1PA4
RC   pRSVCAT-B2 from pRSVcat & linker
RC   pRSVCAT-B2K from pRSVCAT-B2 & linker
RC   pREP4 from pRSVCAT-B2K & pMEP4
RC   pREP5 from pREP4 & oligo
RA   Groger R.K., Morrow D.M., Tykocinski M.L.;
RT   "Directional antisense and sense cDNA cloning using Epstein-Barr
RT   virus episomal expression vectors";
RL   Gene 81:285-294(1989).
XX
RN   [2]
RC   I10CAT from cat gene
RC   HS1CAT from HS1 & cat gene
RC   from pRSVcat & p220.2, EBV
RC   from pSV2-cat & p220.2, EBV
RC   from pSV0-cat & p220.2, EBV
RC   from I10CAT & p220.2, EBV
RC   from HS1CAT & p220.2, EBV
RC   from pLPV-5'-CAT & p220.2, EBV
RA   Hauer C.A., Getty R.R., Tykocinski M.L.;
RT   "Epstein-Barr virus episome-based promoter function in human
RT   myeloid cells";
RL   Nucleic Acids Res. 17:1989-2003(1989).
XX
RN   [3]
RC   lambda hMT-35 from lambda & human MT gene
RC   plasmid from pBR328 & lambda hMT-35
RC   phMTIA-BPV from plasmid & BPV & pBR322
RC   phMTKIA-BPV from plasmid & pV4
RA   Richards R.I., Heguy A., Karin M.;
RT   "Structural and functional analysis of the human
RT   metallothionein-IA gene: differential induction by metal ions and
RT   glucocorticoids";
RL   Cell 37:263-272(1984).
XX
RN   [4]
RC   pdeltapiTK from HAT gene
RC   pTKBamC from pdeltapiTK & EBV oriP
RC   pBamZRSalF from pTKBamC
RC   pSVoB-H2.9 from EBV EBNA-1 gene & SV40
RC   pHEBo2 from pUC12 & EBV oriP & hyg gene & HSV 1 tk gene terminator
RC   p154, p172, p201, p205 from pHEBo2 & EBV EBNA-1 gene
RC   p174 from p154
RC   p220.2 from p201
RA   Yates J.L., Warren N., Sugden B.;
RT   "Stable replication of plasmids derived from Epstein-Barr virus in
RT   various mammalian cells";
RL   Nature 313:812-815(1985).
XX
RN   [5]
RC   pV4 from pBR322 & pTK-46
RA   Majors J.;
RT   ;
RL   Unpublished (1984).
XX
RN   [6]
RC   pLPV-5'-CAT, pLPV-5'-CATs, pLPV-5'-CATa from pA10CAT2 & LPV 5' LTR
RC   pLPV-3'-CATs from pA10CAT2 & LPV 5' LTR
RC   pCmu-5'-CATs, pCmu-5'-CATa from pCAT2-polylinker & mouse Cmu enhancer
RA   Mosthaf L., Pawlita M., Gruss P.;
RT   "A viral enhancer element specifically active in human
RT   haematopoietic cells";
RL   Nature 315:597-600(1985).
XX
RN   [7]
RC   p2I from pUC6.2
RC   pI10 from p2I & pSV0-cat
RA   Resendez E., Attenello J.W., Grafsky A., Chang C.S., Lee A.S.;
RT   "Calcium ionophore A23187 induces expression of glucose-regulated
RT   genes and their heterologous fusion genes";
RL   Mol. Cell. Biol. 5:1212-1219(1985).
XX
RN   [8]
RC   from pTK-46 & hMTIIA gene
RC   pHSI from pTK-109 & hMTIIA gene
RC   pHSI-TK from pHS1 & HSV tk gene
RC   pHSIdeltaX-TK from pHSI-TK & linker
RC   pUCAT2 from pA10CAT2 & pUC8
RC   25.6B/TK-109 from pTK-109 & hMTIIA gene
RC   MTX/TK-109 from pTK-109 & hMTIIA gene
RC   TK-109/MTX from pTK-109 & hMTIIA gene
RC   SVE/TK-109 from SV40 & HSV tk gene
RA   Haslinger A., Karin M.;
RT   "Upstream promoter element of the human metallothionein-IIA gene
RT   can act like an enhancer element";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:8572-8576(1985).
XX
RN   [9]
RC   pTK-CAT from pSV2-cat & HSV-1 tk promoter
RC   pTK-CAT-14C from pTK-CAT & MoMSV LTR
RA   Miksicek R., Heber A., Schmid W., Danesch U., Posseckert G.,
RA   Beato M., Schutz G.;
RT   "Glucocorticoid responsiveness of the transcriptional enhancer of
RT   Moloney murine sarcoma virus";
RL   Cell 46:283-290(1986).
XX
RN   [10]
RC   pNEO3 from neo gene
RC   pNE/12 from pNEO3 & rat GRP78 gene
RC   pUC6.2 from pUC8 & rat GRP78 gene
RA   Attenello J.W., Lee A.S.;
RT   "Regulation of a hybrid gene by glucose and temperature in hamster
RT   fibroblasts";
RL   Science 226:187-190(1984).
XX
RN   [11]
RC   p3C5 from pBR322 & rat GRP78 gene
RC   p4A3 from pBR322 & rat GRP94 gene
RC   p3A10 from pBR322
RA   Lee A.S., Delegeane A.M., Baker V., Chow P.C.;
RT   "Transcriptional regulation of two genes specificaly induced by
RT   glucose starvation in a hamster fibroblast cell line";
RL   J. Biol. Chem. 258:597-603(1983).
XX
RN   [12]
RC   from p205-GTI
RA   Stary A., Sarasin A.;
RT   "Simian virus 40 (SV40) large T antigen-dependent amplification of
RT   an Epstein-Barr virus-SV40 hybrid shuttle vector integrated into
RT   the human HeLa cell genome";
RL   J. Gen. Virol. 73:1679-1685(1992).
XX
RN   [13]
RC   p205-GTI from SV40 & p205, EBV
RA   Stary A., James M.R., Sarasin A.;
RT   "High recombination rate of an Epstein-Barr virus-simian virus 40
RT   hybrid shuttle vector in human cells";
RL   J. Virol. 63:3837-3843(1989).
XX
RN   [14]
RC   pLas from SV40 T antigen/early promoter & amp gene
RA   Daya-Grosjean L., James M.R., Drougard C., Sarasin A.;
RT   "An immortalized xeroderma pigmentosum, group C, cell line which
RT   replicates SV40 shuttle vectors";
RL   Mutat. Res. 183:185-196(1987).
XX
RN   [15]
RC   pKMT11 from SV40 T antigen & mouse MT promoter & kan gene
RA   Gerard R.D., Gluzman Y.;
RT   "New host cell system for regulated simina virus 40 DNA replication";
RL   Mol. Cell. Biol. 5:3231-3240(1985).
XX
RN   [16]
RC   pTK-46 from HSV tk gene
RC   pTK-109 from HSV tk gene
RA   McKnight S.L., Gavis E.R., Kingsbury R., Axel R.;
RT   "Analysis of transcriptional regulatory signals of the HSV
RT   thymidine kinase gene: identification of an upstream control
RT   region";
RL   Cell 25:385-398(1981).
XX
CC   NM (pCEP4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (EBV)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. EBV, EBNA-1
FT                   2. SV40, polyA/tk gene
FT                   3. CMV, immediate early promoter
FT                   4. pBR322 4361bp
FT                   5. E. coli 1142bp, hyg gene
FT                   -> pCEP4 10410bp"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="MCS
FT                   BamHI-SfiI-XhoI-NotI-NheI-HindIII-NheI-PvuII-KpnI"
FT   promoter        complement(0..0)
FT                   /note="PRO human cytomegalovirus (CMV)"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   misc_binding    3142..3142
FT                   /note="SIT NruI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5486..5486
FT                   /note="SIT ClaI"
FT   misc_binding    5632..5632
FT                   /note="SIT StuI"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   misc_binding    9766..9766
FT                   /note="SIT EcoRV"
XX
SQ   Sequence 10410 BP; 2573 A; 2570 C; 2968 G; 2299 T; 0 other;
     tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
     agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
     acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
     ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttct agagatctga
     cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
     aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag
     gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga
     tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     catggcggtc atattggaca tgagccaata taaatgtaca tattatgata tagatacaac
     gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct
     aattgccaat attgattcaa tgtatagatc ttccatacct accagttctg cgcctgcagc
     aatgcaacaa cgttgcccgg atctgcgatg ataagctgtc aaacatgaga attggtcgac
     tagcttggca cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg tttggcagcc
     acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg ccatccaaaa
     accatgggtc tgtctgctca gtccagtcgt ggaccagacc ccacgcaacg cccaaaataa
     taacccccac gaaccataaa ccattcccca tgggggaccc cgtccctaac ccacggggcc
     agtggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccctattcc tttgccctcg
     gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
     cgggatctgc ggcacgctgt tgacgctgtt aagcgggtcg ctgcagggtc gctcggtgtt
     cgaggccaca cgcgtcacct taatatgcga agtggacctg ggaccgcgcc gccccgactg
     catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga
     actaaagaca tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc
     cacggggatg aagcagggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     gccatctcca gcagccgcac gcggcgcagc aaaaggccag gaaccgtaaa aaggccgcgt
     tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc
     atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc cagtagcatc
     tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat ccctaccgtc
     cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg caaatcttga
     ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg aggatggttc
     ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag gggatgaggg
     tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga gcgtcgtcta
     ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg ccaggtacag
     gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc tccggcggca
     gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga ggacgaggac
     gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga catagagatg
     gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc cacggtggaa
     caggagcagg agcaggagcg ggaggggcag gagcaggagg ggcaggagca ggaggagggg
     caggagcagg aggaggggca ggaggggcag gaggggcagg aggggcagga gcaggaggag
     gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag
     gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
     caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag
     gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag
     gggcaggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga ggggcaggag
     gggcaggagc aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag
     gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg
     caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
     caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
     gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga ggtggaggcc
     ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg ggtcgaggag
     gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt cgtgaaagag
     ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt agtcagtcat
     catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt ttccaccctg
     taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt gagcctgacg
     tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc ccaagcactg
     gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt ggaaagcatc
     gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta agagctctcc
     tggctaggag tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc ggtgtgttcg
     tatatggagg tagtaagacc tccctttaca acctaaggcg aggaactgcc cttgctattc
     cacaatgtcg tcttacacca ttgagtcgtc tcccctttgg aatggcccct ggacccggcc
     cacaacctgg cccgctaagg gagtccattg tctgttattt catggtcttt ttacaaactc
     atatatttgc tgaggttttg aaggatgcga ttaaggacct tgttatgaca aagcccgctc
     ctacctgcaa tatcagggtg actgtgtgca gctttgacga tggagtagat ttgcctccct
     ggtttccacc tatggtggaa ggggctgccg cggagggtga tgacggagat gacggagatg
     aaggaggtga tggagatgag ggtgaggaag ggcaggagtg atgtaacttg ttaggagacg
     ccctcaatcg tattaaaagc cgtgtattcc cccgcactaa agaataaatc cccagtagac
     atcatgcgtg ctgttggtgt atttctggcc atctgtcttg tcaccatttt cgtcctccca
     acatggggca attgggcata cccatgttgt cacgtcactc agctccgcgc tcaacacctt
     ctcgcgttgg aaaacattag cgacatttac ctggtgagca atcagacatg cgacggcttt
     agcctggcct ccttaaattc acctaagaat gggagcaacc agcatgcagg aaaaggacaa
     gcagcgaaaa ttcacgcccc cttgggaggt ggcggcatat gcaaaggata gcactcccac
     tctactactg ggtatcatat gctgactgta tatgcatgag gatagcatat gctacccgga
     tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga
     tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     tatagattag gatagcatat gctacccaga tatagattag gatagcatat gctatccaga
     tatttgggta gtatatgcta cccagatata aattaggata gcatatacta ccctaatctc
     tattaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
     gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     gattaggata gcctatgcta cccagatata gattaggata gcatatgcta tccagatatt
     tgggtagtat atgctaccca tggcaacatt agcccaccgt gctctcagcg acctcgtgaa
     tatgaggacc aacaaccctg tgcttggcgc tcaggcgcaa gtgtgtgtaa tttgtcctcc
     agatcgcagc aatcgcgccc ctatcttggc ccgcccacct acttatgcag gtattccccg
     gggtgccatt agtggttttg tgggcaagtg gtttgaccgc agtggttagc ggggttacaa
     tcagccaagt tattacaccc ttattttaca gtccaaaacc gcagggcggc gtgtgggggc
     tgacgcgtgc ccccactcca caatttcaaa aaaaagagtg gccacttgtc tttgtttatg
     ggccccattg gcgtggagcc ccgtttaatt ttcgggggtg ttagagacaa ccagtggagt
     ccgctgctgt cggcgtccac tctctttccc cttgttacaa atagagtgta acaacatggt
     tcacctgtct tggtccctgc ctgggacaca tcttaataac cccagtatca tattgcacta
     ggattatgtg ttgcccatag ccataaattc gtgtgagatg gacatccagt ctttacggct
     tgtccccacc ccatggattt ctattgttaa agatattcag aatgtttcat tcctacacta
     gtatttattg cccaaggggt ttgtgagggt tatattggtg tcatagcaca atgccaccac
     tgaacccccc gtccaaattt tattctgggg gcgtcacctg aaaccttgtt ttcgagcacc
     tcacatacac cttactgttc acaactcagc agttattcta ttagctaaac gaaggagaat
     gaagaagcag gcgaagattc aggagagttc actgcccgct ccttgatctt cagccactgc
     ccttgtgact aaaatggttc actaccctcg tggaatcctg accccatgta aataaaaccg
     tgacagctca tggggtggga gatatcgctg ttccttagga cccttttact aaccctaatt
     cgatagcata tgcttcccgt tgggtaacat atgctattga attagggtta gtctggatag
     tatatactac tacccgggaa gcatatgcta cccgtttagg gttaacaagg gggccttata
     aacactattg ctaatgccct cttgagggtc cgcttatcgg tagctacaca ggcccctctg
     attgacgttg gtgtagcctc ccgtagtctt cctgggcccc tgggaggtac atgtccccca
     gcattggtgt aagagcttca gccaagagtt acacataaag gcaatgttgt gttgcagtcc
     acagcatgca aagtctgctc caggatgaaa gccactcagt gttggcaaat gtgcacatcc
     atttataagg atgtcaacta cagtcagaga acccctttgt gtttggtccc cccccgtgtc
     acatgtggaa cagggcccag ttggcaagtt gtaccaacca actgaaggga ttacatgcac
     tgccccgaat acaaaacaaa agcgctcctc gtaccagcga agaaggggca gagatgccgt
     agtcaggttt agttcgtccg gcggcggggc
//