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ID   PCF20      preliminary; circular DNA; SYN; 4898 BP.
XX
AC   IG0093;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pCF20 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pZf18R from pUC18 & pDM1, f1
RC   pZf18U from pUC18 & pDM1, f1
RC   pZf19R from pUC19 & pDM1, f1
RC   pZf19U from pUC19 & pDM1, f1
RC   pTZ18R from pZf18R & oligo, T7 promoter
RC   pTZ18U from pZf18U & oligo, T7 promoter
RC   pTZ19R from pZf19R & oligo, T7 promoter
RC   pTZ19U from pZf19U & oligo, T7 promoter
RC   pTP6 from pSP64-f1- & pTZ19R
RC   pC2A from pTZ18R & pP-450PBc2
RC   [pCF20 from pTZ18R]
RA   Mead D.A., Szczesna-Skorupa E., Kemper B.;
RT   "Single-stranded DNA blue T7 promoter plasmids: a versatile
RT   tandem promoter system for cloning and protein engineering";
RL   Protein Engineering 1:67-74(1986).
XX
CC   NM (pCF20)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli JM103)(E.coli NM522)
CC   CP ()
CC   FN (cloning)(sequencing)(mutagenesis)(transcription)
CC   SE ()
CC   PA (pTZ18R)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove, 4361bp-
FT                   2. pTZ18R 2861bp
FT                   -> pCF20 4898bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    255..255
FT                   /note="SIT unique EcoRI"
FT   misc_binding    280..280
FT                   /note="SIT unique BanIII"
FT   misc_binding    442..442
FT                   /note="SIT unique EcoRV"
FT   misc_binding    486..486
FT                   /note="SIT unique NheI"
FT   misc_binding    632..632
FT                   /note="SIT unique BamHI"
FT   misc_binding    879..879
FT                   /note="SIT unique EcoNI"
FT   misc_binding    908..908
FT                   /note="SIT unique SalI"
FT   misc_binding    1196..1196
FT                   /note="SIT unique Eco52I"
FT   misc_binding    1229..1229
FT                   /note="SIT unique NruI"
FT   misc_binding    1610..1610
FT                   /note="SIT unique BsmI"
FT   misc_binding    1626..1626
FT                   /note="SIT unique StyI"
FT   misc_binding    1682..1682
FT                   /note="SIT unique AvaI"
FT   misc_binding    1701..1701
FT                   /note="SIT unique MscI"
FT   misc_binding    1921..1921
FT                   /note="SIT unique MroI"
FT   misc_binding    1925..1925
FT                   /note="SIT unique BsaBI"
FT   misc_binding    2332..2332
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   misc_binding    2730..2730
FT                   /note="SIT unique DraIII"
FT   misc_binding    2733..2733
FT                   /note="SIT unique BsaAI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3402..3402
FT                   /note="SIT unique ScaI"
FT   misc_binding    3813..3813
FT                   /note="SIT unique BsaI"
FT   misc_binding    4359..4359
FT                   /note="SIT unique AlwNI"
FT   rep_origin      2615..2615
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    4773..4773
FT                   /note="SIT unique AflIII"
XX
SQ   Sequence 4898 BP; 1139 A; 1318 C; 1287 G; 1154 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaatttaata
     cgactcacta tagggaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
     agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
     tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
     tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc
     ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     ggcccgttgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
     gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
     ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca
     tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
     cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
     gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
     tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
     tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
     cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
     gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
     tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat tcccccttac
     acggaggcat caagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
     aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
     catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgga cctgcaggca
     tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg aaattgtaaa
     cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     gaccgagcgc agcgagtcag tgagcgagga agcggaag
//