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ID PCF20 preliminary; circular DNA; SYN; 4898 BP.
XX
AC IG0093;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pCF20 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pZf18R from pUC18 & pDM1, f1
RC pZf18U from pUC18 & pDM1, f1
RC pZf19R from pUC19 & pDM1, f1
RC pZf19U from pUC19 & pDM1, f1
RC pTZ18R from pZf18R & oligo, T7 promoter
RC pTZ18U from pZf18U & oligo, T7 promoter
RC pTZ19R from pZf19R & oligo, T7 promoter
RC pTZ19U from pZf19U & oligo, T7 promoter
RC pTP6 from pSP64-f1- & pTZ19R
RC pC2A from pTZ18R & pP-450PBc2
RC [pCF20 from pTZ18R]
RA Mead D.A., Szczesna-Skorupa E., Kemper B.;
RT "Single-stranded DNA blue T7 promoter plasmids: a versatile
RT tandem promoter system for cloning and protein engineering";
RL Protein Engineering 1:67-74(1986).
XX
CC NM (pCF20)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (non-coding)
CC TY (phagemid)
CC SP (USB)
CC HO (E.coli JM103)(E.coli NM522)
CC CP ()
CC FN (cloning)(sequencing)(mutagenesis)(transcription)
CC SE ()
CC PA (pTZ18R)(pBR322)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 remove, 4361bp-
FT 2. pTZ18R 2861bp
FT -> pCF20 4898bp"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 255..255
FT /note="SIT unique EcoRI"
FT misc_binding 280..280
FT /note="SIT unique BanIII"
FT misc_binding 442..442
FT /note="SIT unique EcoRV"
FT misc_binding 486..486
FT /note="SIT unique NheI"
FT misc_binding 632..632
FT /note="SIT unique BamHI"
FT misc_binding 879..879
FT /note="SIT unique EcoNI"
FT misc_binding 908..908
FT /note="SIT unique SalI"
FT misc_binding 1196..1196
FT /note="SIT unique Eco52I"
FT misc_binding 1229..1229
FT /note="SIT unique NruI"
FT misc_binding 1610..1610
FT /note="SIT unique BsmI"
FT misc_binding 1626..1626
FT /note="SIT unique StyI"
FT misc_binding 1682..1682
FT /note="SIT unique AvaI"
FT misc_binding 1701..1701
FT /note="SIT unique MscI"
FT misc_binding 1921..1921
FT /note="SIT unique MroI"
FT misc_binding 1925..1925
FT /note="SIT unique BsaBI"
FT misc_binding 2332..2332
FT /note="SIT unique PstI"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT misc_binding 2730..2730
FT /note="SIT unique DraIII"
FT misc_binding 2733..2733
FT /note="SIT unique BsaAI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3402..3402
FT /note="SIT unique ScaI"
FT misc_binding 3813..3813
FT /note="SIT unique BsaI"
FT misc_binding 4359..4359
FT /note="SIT unique AlwNI"
FT rep_origin 2615..2615
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 4773..4773
FT /note="SIT unique AflIII"
XX
SQ Sequence 4898 BP; 1139 A; 1318 C; 1287 G; 1154 T; 0 other;
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaatttaata
cgactcacta tagggaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc
ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
ggcccgttgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca
tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat tcccccttac
acggaggcat caagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgga cctgcaggca
tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg aaattgtaaa
cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
gaccgagcgc agcgagtcag tgagcgagga agcggaag
//