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ID   PCH110     preliminary; circular DNA; SYN; 7128 BP.
XX
AC   IG1053;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCH110 or pSV-lacZ - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCH101 from pMC931 & pCH6
RC   pCH102 from pMDSG & pCH101
RC   pSV2-gpt/HindIII-BglIIdelta from pSV2-gpt
RC   pCH104 from pSV2-gpt/HindIII-BglIIdelta & pCH101
RC   pCH105 from pCH104 & MMTV
RC   pCH110 or pSV-lacZ from pCH104
RC   pCH111 from pCH104
RA   Hall C.V., Jacob P.E., Ringold G.M., Lee F.;
RT   "Expression and regulation of Escherichia coli lacZ gene fusions
RT   in mammalian cells";
RL   J. Mol. Appl. Genet. 2:101-109(1983).
XX
RN   [2]
RC   [from none]
RA   Miller J.H.;
RT   ;
RL   Experiments in Molecular Genetics 0:352-355(1972).
RL   Miller J.H., Cold Spring Harbor Laboratory, New York.
XX
RN   [3]
RC   pSB1bacteria from pSV2-cat & pML2
RC   pCOL-CAT, pCalpha-CAT from chicken collagen alpha2 gene & pSB1
RA   Herbomel P., Bourachot B., Yaniv M.;
RT   "Two distinct enhancers with different cell specificities coexist
RT   in the regulatory region of polyoma";
RL   Cell 39:653-662(1984).
XX
RN   [4]
RC   from pSV2-gpt
RC   from pCH110 or pSV-lacZ
RC   from pMT11
RA   Lee F., Hall C.V., Ringold G.M., Dobson D.E., Luh J., Jacob P.E.;
RT   "Functional analysis of the steroid hormone control region of
RT   mouse mammary tumor virus";
RL   Nucleic Acids Res. 12:4191-4206(1984).
XX
RN   [5]
RC   pSC13-42 from trpS promoter
RC   pCH3 from pBR322 & pSC13-42, trpS promoter
RC   pCH6 from pCH3
RC   pCH17 from pCH6
RC   pCH22 from pMC931 & pCH6, trpS promoter
RA   Hall C.V., Yanofsky C.;
RT   "Cloning and characterization of the gene for Escherichia coli
RT   tryptophanyl-transfer ribonucleic acid synthetase";
RL   J. Bacteriol. 148:941-949(1981).
XX
RN   [6]
RC   pFJ1 from pCH110 & linker
RC   pFJ3 from pFJ1 & oligo
RC   pGX40Xba from pGX40 & linker
RC   pGX40Gal from pGX40Xba & pFJ3
RC   from pGX40Gal
RC   pFJ5 from pFJ3 & pTER5, HSV-2 immediate early 5 polyA
RC   pFJ5deltaHind from pFJ5
RC   pFJ7 from pFJ5deltaHind & HSV-2 RR2 promoter
RC   pLW51 from pLW5
RC   pFJ10 from pFJ5deltaHind & pLW51
RC   vFJ7, vFJ7tsK from pFJ7
RC   vFJ10, vFJ10tsK from pFJ10
RA   Rixon F.J., McLauchlan J.;
RT   "Insertion of DNA sequences at a unique restriction enzyme site
RT   engineered for vector purposes into the genome of herpes simplex
RT   virus type 1";
RL   J. Gen. Virol. 71:2931-2939(1990).
XX
RN   [7]
RC   pGX35, pGX40, pGX43, pGX45, pGX55, pGX156 from pAT153 & HSV-1 genome
RC   pGX168, pGX169 from pAT153 & HSV-1 genome
RA   Rixon F.J., McGeoch D.J.;
RT   "Detailed analysis of the mRNAs mapping in the short unique region
RT   of herpes simplex virus type 1";
RL   Nucleic Acids Res. 13:953-973(1985).
XX
RN   [8]
RC   pTK1 from pAT153 & HSV TK gene
RC   pTK6 from pTK1
RA   Preston C.M., McGeoch D.J.;
RT   "Identification and mapping of two polypeptides encoded within the
RT   herpes simplex virus type 1 thymidine kinase gene sequences";
RL   J. Virol. 38:593-605(1981).
XX
RN   [9]
RC   pUC8CAT from pUC8 & cat gene
RC   pLW1 from pUC8CAT & HSV-2 IE gene 4/5 promoter
RC   pDGC13 from pLW1 & HSV-2 IE gene 5 terminator
RC   pLW2 from pDGC13 & pUC9
RC   pLW3 from pLW2 & SV40 72bp repeat
RC   pLW4, pLW5 from pLW2 & HSV-1 activator
RC   pSYN1, pSYN2, pSYN3, pSYN4, pSYN5 from pLW2 & oligo
RA   Gaffney D.F., McLauchlan J., Whitton J.L., Clements J.B.;
RT   "A modular system for th eassay of transcription regulatory signals:
RT   the sequence TAATGARAT is required for herpes simplex virus immediate
RT   early gene activation";
RL   Nucleic Acids Res. 13:7847-7863(1985).
XX
RN   [10]
RC   pGX1 from pAT153 & HSV-1 ts, Vmw 175 gene
RC   pGX13 from pAT153 & HSV-1 ts K, Vmw 175 gene
RC   pGX14, pGX15 from pAT153 & HSV-1 ts K revertant, Vmw 175 gene
RA   Davison M.J., Preston V.G., McGeoch D.J.;
RT   "Determination of the sequence alteration in the DNA of the herpes
RT   simplex virus type 1 temperature-sensitive mutant ts K";
RL   J. Gen. Virol. 65:859-863(1984).
XX
RN   [11]
RC   pMT11 from MMTV
RA   Huang H., Moore K.;
RT   ;
RL   Unpublished (1984).
XX
CC   NM (pCH110)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)(monkey COS cells)
CC   CP ()
CC   FN (expression shuttle)(promoter analysis)
CC   SE ()
CC   PA (pBR322)(SV40)(E.coli lacZ)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1 6646bp
FT                   2. E. coli, trpS gene
FT                   -> pSC13-42 30000bp
FT                   1. pBR322 BamHI 4361bp 376..376
FT                   2. pSC13-42 BamHI-BamHI 3800bp, trpS gene
FT                   -> pCH3 8200bp
FT                   1. pCH3 BamHI-EcoRI 3800bp, trpS gene
FT                   2. pCH3 BamHI-EcoRI 4000bp, pBR322
FT                   -> pCH6 7800bp
FT                   1. ColE1 6646bp
FT                   2. E. coli, lacZ gene
FT                   -> pMC81
FT                   1. pMC81
FT                   2. pACYC177
FT                   -> pMC275
FT                   1. pMC275
FT                   -> pMC874 [kan, unique BamHI in lacZ]
FT                   1. ColE1 6646bp
FT                   2. Tn3 BspHI-BspHI 1008bp 3898..4906, amp gene/#V00613
FT                   -> pACYC999
FT                   1. pMC874, lacZ
FT                   2. pACYC999, p15A ori
FT                   -> pMC931
FT                   1. pMC931
FT                   2. pCH6
FT                   -> pCH22 14600bp
FT                   1. pCH22 BglII 14600bp, 3500bp 3' to lacZ
FT                   2. pCH22 HaeII-HaeII 200bp, KpnI
FT                   -> pCH101 14800bp
FT                   1. pSV2-gpt BglII 5500bp
FT                   HindIII linker 6bp aagctt
FT                   -> pSV2-gpt/HindIII-BglIIdelta 5000bp
FT                   1. pSV2-gpt/HindIII-BglIIdelta KpnI 5000bp
FT                   2. pCH101 KpnI-KpnI 7000bp, lacZ gene
FT                   -> pCH104 12000bp
FT                   1. pCH104 remove HpaI-AvaI 5000bp,
FT                   \ SV40 splice/5' lacY gene
FT                   -> pCH110 7128bp"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   misc_binding    1..1
FT                   /note="SIT unique HindIII"
FT   CDS             0..0
FT                   /note="GEN E. coli xanthine-guanine
FT                   phosphoribosyl-transferase (gpt) gene (40 codons)"
FT   misc_feature    0..0
FT                   /note="ATG E. coli gpt gene start codon"
FT   misc_binding    204..204
FT                   /note="SIT unique KpnI"
FT   CDS             0..0
FT                   /note="GEN E. coli trpS gene (38 codons)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    3736..3736
FT                   /note="SIT unique BamHI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 7128 BP; 1710 A; 1849 C; 1817 G; 1752 T; 0 other;
     aagcttgatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     attgccgtaa gccgtggcgg tctggtaccg gtgggtgaag accagaaaca gcacctcgaa
     ctgagccgcg atattgccca gcgtttcaac gcgctgtatg gcgagatcga tcccgtcgtt
     ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
     cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
     ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
     gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
     catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa
     tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt
     tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
     gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
     tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
     cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
     accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca
     ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
     gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc
     ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
     attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
     acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
     cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg
     gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg
     cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
     ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg
     ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga
     gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
     cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
     cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct
     aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg
     gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt
     gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac
     cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt
     ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
     ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg
     gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
     gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
     gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
     cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag
     ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt
     ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
     aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
     aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt
     gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt
     atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
     gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag
     gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
     actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
     ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
     tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
     agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat
     atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc
     gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca
     tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa cacaaacttt
     tggatgttcg gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt
     ttcccgattt ggctacatga catcaaccat atcagcaaaa gtgatacggg tattattttt
     gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa
     ctcggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     tatcttatca tgtctggatc cccaggaagc tcctctgtgt cctcataaac cctaacctcc
     tctacttgag aggacattcc aatcataggc tgcccatcca ccctctgtgt cctcctgtta
     attaggtcac ttaacaaaaa ggaaattggg taggggtttt tcacagaccg ctttctaagg
     gtaattttaa aatatctggg aagtcccttc cactgctgtg ttccagaagt gttggtaaac
     agcccacaaa tgtcaacagc agaaacatac aagctgtcag ctttgcacaa gggcccaaca
     ccctgctcat caagaagcac tgtggttgct gtgttagtaa tgtgcaaaac aggaggcaca
     ttttccccac ctgtgtaggt tccaaaatat ctagtgtttt catttttact tggatcagga
     acccagcact ccactggata agcattatcc ttatccaaaa cagccttgtg gtcagtgttc
     atctgctgac tgtcaactgt agcatttttt ggggttacag tttgagcagg atatttggtc
     ctgtagtttg ctaacacacc ctgcagctcc aaaggttccc caccaacagc aaaaaaatga
     aaatttgacc cttgaatggg ttttccagca ccattttcat gagttttttg tgtccctgaa
     tgcaagttta acatagcagt taccccaata acctcagttt taacagtaac agcttcccac
     atcaaaatat ttccacaggt taagtcctca tttaaattag gcaaaggaat tcttgaagac
     gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagct
//