back Return to this vector's summary.
ID   PCHL1      preliminary; circular DNA; SYN; 7499 BP.
XX
AC   A13313; A13314;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pCHL1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7499
RC   pCHL1 from pBR322
RA   Ratti G., Comanducci M., Ricci S., Garuti G., Cosco E.;
RT   "Synthetic oligonucleotides useful for the determination of
RT   Chlamydia trachomatis in a biological sample";
RL   Unpublished (1986).
RL   European Patent No. 0336412-A 24 dated 11-OCT-1989.
RL   SCLAVO S.p.A.
XX
CC   NCBI gi: 583024
CC   NM (pCHL1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pCHL1 7499bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(88..1080)
FT                   /note="GEN orf 8; NCBI gi: 583025"
FT   CDS             1179..2534
FT                   /note="GEN orf 1; NCBI gi: 583026"
FT   CDS             2528..3592
FT                   /note="GEN orf 2; NCBI gi: 583027"
FT   CDS             3654..4448
FT                   /note="GEN orf 3; NCBI gi: 583028"
FT   CDS             4518..4826
FT                   /note="GEN orf 4; NCBI gi: 583029"
FT   CDS             4917..5648
FT                   /note="GEN orf 5; NCBI gi: 583030"
FT   CDS             5645..6388
FT                   /note="GEN orf 6; NCBI gi: 583031"
FT   CDS             6620..7402
FT                   /note="GEN orf 7; NCBI gi: 583032"
XX
SQ   Sequence 7499 BP; 2454 A; 1284 C; 1433 G; 2328 T; 0 other;
     ggtaagtcct ctagtacaaa cacccccaat attgtgatat aattaaaatt atattcatat
     tctgttgcca gaaaaaacac ttttaggcta tattagagcc atcttctttg aagcgttgtc
     ttctcgagaa gatttatcgt acgcaaatat catctttgcg gttgcgtgtc ctgtgacctt
     cattatgtcg gagtctgagc accctaggcg tttgtactcc gtcacagcgg ttgctcgaag
     cacgtgcggg gttatcttaa aagggattgc agcttgtagt cctgcttgag agaacgtgcg
     ggcgatttgc cttaacccca ccatttttcc ggagcgagtt acgaagacaa aacctcttcg
     ttgaccgatg tactcttgta gaaagtgcat aaacttctga ggataagtta taataatcct
     cttttctgtc tgacggttct taagctggga gaaagaaatg gtagcttgtt ggaaacaaat
     ctgactaatc tccaagctta agacttcaga ggagcgttta cctccttgga gcattgtctg
     ggcgatcaac caatcccggg cattgatttt ttttagctct tttaggaagg acgctgtttg
     caaactgttc atcgcatctg tttttactat ttccctggtt ttaaaaaatg ttcgactatt
     ttcttgttta gaaggttgcg ctatagcgac tattccttga gtcatcctgt ttaggaatct
     tgttaaggaa atatagcttg ctgctcgaac ttgtttagta ccttcggtcc aagaagtctt
     ggcagaggaa acttttttaa tcgcatctag aattagatta tgatttaaaa gggaaaactc
     ttgcagattc atatccaagg acaatagacc aatcttttct aaagacaaaa aagatcctcg
     atatgatcta caagtatgtt tgttgagtga tgcggtccaa tgcataataa cttcgaataa
     ggagaagctt ttcatgcgtt tccaatagga ttcttggcga atttttaaaa cttcctgata
     agacttttcg ctatattcta acgacatttc ttgctgcaaa gataaaatcc ctttacccat
     gaaatccctc gtgatataac ctatccgtaa aatgtcctga ttagtgaaat aatcaggttg
     ttaacaggat agcacgctcg gtattttttt atataaacat gaaaactcgt tccgaaatag
     aaaatcgcat gcaagatatc gagtatgcgt tgttaggtaa agctctgata tttgaagact
     ctactgagta tattctgagg cagcttgcta attatgagtt taagtgttct catcataaaa
     acatattcat agtatttaaa tacttaaaag acaatggatt acctataact gtagactcgg
     cttgggaaga gcttttgcgg cgtcgtatca aagatatgga caaatcgtat ctcgggttaa
     tgttgcatga tgctttatca aatgacaagc ttagatccgt ttctcatacg gttttcctcg
     atgatttgag cgtgtgtagc gctgaagaaa atttgagtaa tttcattttc cgctcgttta
     atgagtacaa tgaaaatcca ttgcgtagat ctccgtttct attgcttgag cgtataaagg
     gaaggcttga cagtgctata gcaaagactt tttctattcg cagcgctaga ggccggtcta
     tttatgatat attctcacag tcagaaattg gagtgctggc tcgtataaaa aaaagacgag
     caacgttctc tgagaatcaa aattctttct ttgatgcctt cccaacagga tacaaggata
     ttgatgataa aggagttatc ttagctaaag gtaatttcgt gattatagca gctaggccat
     ctatagggaa aactgcttta gctatagaca tggcgataaa tcttgcggtt actcaacagc
     gtagagttgg tttcctatct ctagaaatga gcgcaggtca aattgttgag cggattattg
     ctaatttaac aggaatatct ggtgaaaaat tacaaagagg ggatctctct aaagaagaat
     tattccgagt agaagaagct ggagaaacag ttagagaatc acatttttat atctgcagtg
     atagtcagta taagcttaat ttaatcgcga atcagatccg gttgctgaga aaagaagatc
     gagtagacgt aatatttatc gattacttgc agttgatcaa ctcatcggtt ggagaaaatc
     gtcaaaatga aatagcagat atatctagaa ccttaagagg tttagcctca gagctaaaca
     ttcctatagt ttgtttatcc caactatcta gaaaagttga ggatagagca aataaagttc
     ccatgctttc agatttgcga gacagcggtc aaatagagca agacgcagat gtgattttgt
     ttatcaatag gaaggaatcg tcttctaatt gtgagataac tgttgggaaa aatagacatg
     gatcggtttt ctcttcggta ttacatttcg atccaaaaat tagtaaattc tccgctatta
     aaaaagtatg gtaaattata gtaactgcca cttcatcaaa agtcctatcc accttgaaaa
     tcagaagttt ggaagaagac ctggtcaatc tattaagata tctcccaaat tggctcaaaa
     tgggatggta gaagttatag gtcttgattt tctttcatct cattaccatg cattagcagc
     tatccaaaga ttgctgactg caacgaatta caaggggaac acaaaagggg ttgttttatc
     cagagaatca aatagttttc aatttgaagg atggatacca agaatccgtt ttacaaaaac
     tgaattctta gaggcttatg gagttaagcg gtataaaaca tccagaaata agtatgagtt
     tagtggaaaa gaagctgaaa ctgctttaga agccttatac catttaggac atcaaccgtt
     tttaatagtg gcaactagaa ctcgatggac taatggaaca caaatagtag accgttacca
     aactctttct ccgatcatta ggatttacga aggatgggaa ggtttaactg acgaagaaaa
     tatagatata gacttaacac cttttaattc accatctaca cggaaacata aagggttcgt
     tgtagagcca tgtcctatct tggtagatca aatagaatcc tactttgtaa tcaagcctgc
     aaatgtatac caagaaataa aaatgcgctt cccaaatgca tcaaagtatg cttacacatt
     tatcgactgg gtgattacag cagctgcgaa aaagagacga aaattaacta aggataattc
     ttggccagaa aacttgttct taaacgttaa cgttaaaagt cttgcatata ttttaaggat
     gaatcggtac atttgtacaa ggaactggaa aaaaatcgag ttagctatcg ataaatgtat
     agaaatcgcc attcagcttg gttggttatc tagaagaaaa cgcattgaat ttctggattc
     ttctaaactc tctaaaaaag aaattctata tctaaataaa gagcgttttg aagaaataac
     taagaaatct aaagaacaaa tggaacaatt agaacaagaa tctattaatt aatagcaaac
     ttgaaactaa aaacctaatt tatttaaagc tcaaaataaa aaagagtttt aaaatgggaa
     attctggttt ttatttgtat aacactcaaa actgcgtctt tgctgataat atcaaagttg
     ggcaaatgac agagccgctc aaggaccagc aaataatcct tgggacaaca tcaacacctg
     tcgcagccaa aatgacagct tctgatggaa tatctttaac agtctccaat aatccatcaa
     ccaatgcttc tattacaatt ggtttggatg cggaaaaagc ttaccagctt attctagaaa
     agttgggaga tcaaattctt ggtggaattg ctgatactat tgttgatagt acagtccaag
     atattttaga caaaatcaca acagaccctt ctctaggttt gttgaaagct tttaacaact
     ttccaatcac taataaaatt caatgcaacg ggttattcac tcccaggaac attgaaactt
     tattaggagg aactgaaata ggaaaattca cagtcacacc caaaagctct gggagcatgt
     tcttagtctc agcagatatt attgcatcaa gaatggaagg cggcgttgtt ctagctttgg
     tacgagaagg tgattctaag ccctacgcga ttagttatgg atactcatca ggcgttccta
     atttatgtag tctaagaacc agaattatta atacaggatt gactccgaca acgtattcat
     tacgtgtagg cggtttagaa agcggtgtgg tatgggttaa tgccctttct aatggcaatg
     atattttagg aataacaaat acttctaatg tatctttttt ggaggtaata cctcaaacaa
     acgcttaaac aatttttatt ggatttttct tataggtttt atatttagag aaaaaagttc
     gaattacggg gtttgttatg caaaataaaa gcaaagtgag ggacgatttt attaaaattg
     ttaaagatgt gaaaaaagat ttccccgaat tagacctaaa aatacgagta aacaaggaaa
     aagtaacttt cttaaattct cccttagaac tctaccataa aagtgtctca ctaattctag
     gactgcttca acaaatagaa aactctttag gattattccc agactctcct gttcttgaaa
     aattagagga taacagttta aagctaaaaa aggctttgat tatgcttatc ttgtctagaa
     aagacatgtt ttccaaggct gaatagataa cttactctaa cgttggagtt gatttgcaca
     ccttagtttt ttgctctttt aagggaggaa ctggaaaaac aacactttct ctaaacgtgg
     gatgcaactt ggcccaattt ttagggaaaa aagtgttact tgctgaccta gacccgcaat
     ccaatttatc ttctggattg ggggctagtg tcagaagtaa ccaaaaaggc ttacacgaca
     tagtatacac atcaaacgat ttaaaatcaa tcatttgcga aacaaaaaaa gatagtgtgg
     acctaattcc tgcatcattt ttatccgaac agtttagaga attggatatt catagaggac
     ctagtaacaa cttaaagtta tttctgaatg agtactgcgc tcctttttat gacatctgca
     taatagacac tccacctagc ctaggagggt taacgaaaga agcttttgtt gcaggagaca
     aattaattgc ttgtttaact ccagaacctt tttctattct agggttacaa aagatacgtg
     aattcttaag ttcggtcgga aaacctgaag aagaacacat tcttggaata gctttgtctt
     tttgggatga tcgtaactcg actaaccaaa tgtatataga cattatcgag tctatttaca
     aaaacaagct tttttcaaca aaaattcgtc gagatatttc tctcagccgt tctcttctta
     aagaagattc tgtagctaat gtctatccaa attctagggc cgcagaagat attctgaagt
     taacgcatga aatagcaaat attttgcata tcgaatatga acgagattac tctcagagga
     caacgtgaac aaactaaaaa aagaagcgaa tgtctttttt aaaaaaaatc aaactgccgc
     ttctttagat tttaagaaga cgcttccttc cattgaacta ttctcagcaa ctttgaattc
     tgaggaaagt cagagtttgg atcaattatt tttatcagag tcccaaaact attcggatga
     agaattttat caagaagaca tcctagcggt aaaactgctt actggtcaga taaaatccat
     acagaagcaa cacgtacttc ttttaggaga aaaaatctat aatgctagaa aaatcctgag
     taaggatcac ttctcctcaa caactttttc atcttggata gagttagttt ttagaactaa
     gtcttctgct tacaatgctc ttgcatatta cgagcttttt ataaacctcc ccaaccaaac
     tctacaaaaa gagtttcaat cgatccccta taaatccgca tatattttgg ccgctagaaa
     aggcgattta aaaaccaagg tcgatgtgat agggaaagta tgtggaatgt cgaactcatc
     ggcgataagg gtgttggatc aatttcttcc ttcatctaga aacaaagacg ttagagaaac
     gatagataag tctgattcag agaagaatcg ccaattatct gatttcttaa tagagatact
     tcgcatcatg tgttccggag tttctttgtc ctcctataac gaaaatcttc tacaacagct
     ttttgaactt tttaagcaaa agagctgatc ctccgtcagc tcatatatat atctattata
     tatatatatt tagggatttg attttacgag agagatttgc aactcttggt ggtagacttt
     gcaactcttg gtggtagact ttgcaactct tggtggtaga ctttgcaact cttggtggta
     gacttggtca taatggactt ttgttgaaaa atttcttaaa atcttagagc tccgattttg
     aatagctttg gttaagaaaa tgggctcgat ggctttccat aaaagtaggt tgttcttaac
     ttttggggac gcgtcggaaa tttggttatc tactttatct catctaacta gaaaaaatta
     tgcgtctggg attaactttc ttgtttcttt agagattctg gatttatcgg aaaccttgat
     aaaggctatt tctcttgacc acagcgaatc tttgtttaaa atcaagtctc tagatgtttt
     taatggaaaa gtcgtttcag aggcctctaa acaggctaga gcggcatgct acatatcttt
     cacaaagttt ttgtatagat tgaccaaggg atatattaaa cccgctattc cattgaaaga
     ttttggaaac actacatttt ttaaaatccg agacaaaatc aaaacagaat cgatttctaa
     gcaggaatgg acagtttttt ttgaagcgct ccggatagtg aattatagag actatttaat
     cggtaaattg attgtacaag ggatccgtaa gttagacgaa attttgtctt tgcgcacaga
     cgatctattt tttgcatcca atcagatttc ctttcgcatt aaaaaaagac agaataaaga
     aaccaaaatt ctaatcacat ttcctatcag cttaatggag gagttgcaaa aatacacttg
     tgggagaaat gggagagtat ttgtttctaa aatagggatt cctgtaacaa caagtcaggt
     tgcgcataat tttaggcttg cagagttcta tagtgctatg aaaaaaaaat tactcctaga
     gtacttcgtg caagcgcttt gattcattta aagcaaatag gattaaaaga tgaggaaatc
     atgcgtattt cctgtctttc atcgagacaa agtgtgtgtt cttattgttc tggggaaga
//