The pCI and pSI Mammalian Expression Vectors are designed to promote constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the two vectors is the enhancer/promoter region controlling the expression of the inserted gene. The pSI Expression Vector contains the simian virus 40 (SV40) enhancer and early promoter region, whereas the pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region.

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LOCUS       CVU47119     4008 bp    DNA   circular  SYN       20-FEB-1996
DEFINITION  Cloning vector pCI, mammalian expression vector, complete sequence.
ACCESSION   U47119
NID         g1197672
KEYWORDS    .
SOURCE      Cloning vector pCI.
  ORGANISM  Cloning vector pCI
            artificial sequence; cloning vectors.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Brondyk,W.H. and Groskreutz,D.J.
  TITLE     pCI and pSI Mammalian Expression Vectors
  JOURNAL   Promega Notes 49, 7-11 (1994)
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Brondyk,W.H. and Groskreutz,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
COMMENT
            NCBI gi: 1197672
FEATURES               Location/Qualifiers
     source          1..4008
                     /organism="Cloning vector pCI"
     promoter        1..795
                     /note="Cytomegalovirus immediate-early enhancer/promoter
                     region"
     intron          857..989
                     /note="chimeric intron"
     promoter        1034..1052
                     /note="T7 promoter"
     misc_feature    1051
                     /note="T7 promoter transcription start site"
     misc_feature    1052..1104
                     /note="multiple cloning sites"
     polyA_signal    1111..1332
                     /note="SV40 late polyadenylation signal"
     rep_origin      1422..1877
                     /note="phage f1 region"
     CDS             2316..3176
                     /gene="ampR"
                     /note="NCBI gi: 1197673"
                     /codon_start=1
                     /function="ampicillin resistance"
                     /product="beta-lactamase"
                     /db_xref="PID:g1197673"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT     1017 a    956 c    957 g   1078 t
ORIGIN      
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc
     1081 tctagagtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     1141 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1201 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1261 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     1321 acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa tagcgaagag gcccgcaccg
     1381 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
     1441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1561 tcaagctcta aatcgggggc tccctttagg gttccgattt agagctttac ggcacctcga
     1621 ccgcaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1741 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     1801 ggcctattgg ttaaaaaatg agctgattta acaaatattt aacgcgaatt ttaacaaaat
     1861 attaacgttt acaatttcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     1921 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     1981 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     2041 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     2101 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     2161 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3481 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3961 ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatct
//