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LOCUS CVU47119 4008 bp DNA circular SYN 20-FEB-1996
DEFINITION Cloning vector pCI, mammalian expression vector, complete sequence.
ACCESSION U47119
NID g1197672
KEYWORDS .
SOURCE Cloning vector pCI.
ORGANISM Cloning vector pCI
artificial sequence; cloning vectors.
REFERENCE 1 (bases 1 to 4008)
AUTHORS Brondyk,W.H. and Groskreutz,D.J.
TITLE pCI and pSI Mammalian Expression Vectors
JOURNAL Promega Notes 49, 7-11 (1994)
REFERENCE 2 (bases 1 to 4008)
AUTHORS Brondyk,W.H. and Groskreutz,D.J.
TITLE Direct Submission
JOURNAL Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation,
5445 East Cheryl Parkway, Madison, WI 53711, USA
COMMENT
NCBI gi: 1197672
FEATURES Location/Qualifiers
source 1..4008
/organism="Cloning vector pCI"
promoter 1..795
/note="Cytomegalovirus immediate-early enhancer/promoter
region"
intron 857..989
/note="chimeric intron"
promoter 1034..1052
/note="T7 promoter"
misc_feature 1051
/note="T7 promoter transcription start site"
misc_feature 1052..1104
/note="multiple cloning sites"
polyA_signal 1111..1332
/note="SV40 late polyadenylation signal"
rep_origin 1422..1877
/note="phage f1 region"
CDS 2316..3176
/gene="ampR"
/note="NCBI gi: 1197673"
/codon_start=1
/function="ampicillin resistance"
/product="beta-lactamase"
/db_xref="PID:g1197673"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1017 a 956 c 957 g 1078 t
ORIGIN
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc
1081 tctagagtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
1141 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
1201 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
1261 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
1321 acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa tagcgaagag gcccgcaccg
1381 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
1441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
1501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
1561 tcaagctcta aatcgggggc tccctttagg gttccgattt agagctttac ggcacctcga
1621 ccgcaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
1681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
1741 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
1801 ggcctattgg ttaaaaaatg agctgattta acaaatattt aacgcgaatt ttaacaaaat
1861 attaacgttt acaatttcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
1921 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
1981 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
2041 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
2101 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
2161 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
2221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
2341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
2401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
2461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
2521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
2581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
2641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
2701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
2761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
2821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
2881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
2941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
3001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
3061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
3121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
3181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
3241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
3301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
3361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
3421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
3481 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
3541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
3601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
3661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
3721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
3781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
3841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
3901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
3961 ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatct
//