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ID   PCKSP6     preliminary; circular DNA; SYN; 2966 BP.
XX
AC   L08861; VB0121;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCKSP6 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2966
RC   pCKSP6
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [2]
RC   pCKSP6 from pSP64
RC   pCKSP6d3, pCKSP6d4, pCKSP6d5, pCK-X, pCK+X, pCK+Xd from pCKSP6
RA   Kang C., Wu C.W.;
RT   "Studies on SP6 promoter using a new plasmid vector that allows
RT   gene insertion at the transcription initiation site";
RL   Nucleic Acids Res. 15:2279-2294(1987).
XX
RN   [3]
RC   pXbs201 from pBR322 & pXbsdelta5'-50
RC   from pXbs201
RA   Bogenhagen D.F., Sakonju S., Brown D.D.;
RT   "A control region in the center of the 5S RNA gene directs
RT   specific initiation of transcription: II. The 3' border of the
RT   region";
RL   Cell 19:27-35(1980).
XX
RN   [4]
RC   lambda 3a1.b from lambda gt10 & X. laevis TFIIIA gene
RC   pUC3a1.b from pUC13 & lambda 3a1.b
RA   Ginsberg A.M., King B.O., Roeder R.G.;
RT   "Xenopus 5S gene transcription factor, TFIIIA: characterization of
RT   a cDNA clone and measurement of RNA levels throughout
RT   development";
RL   Cell 39:479-489(1984).
XX
RN   [5]
RC   pXbs1 from pBR322 & X. borealis somatic 5S RNA gene
RC   pXbsdelta5'-50 from pBR322 & linker & pXbs1, X. borealis 5S RNA gene
RA   Sakonju S., Bogenhagen D.F., Brown D.D.;
RT   "A control region in the center of the 5S RNA gene directs
RT   specific initiation of transcription: I. The 5' border of the
RT   region";
RL   Cell 19:13-25(1980).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pSP64 according to the cloning strategy by F.
CC   Pfeiffer.
CC   Deletion of some bases from pSP64 brings the BamHI site directly
CC   to the SP6 RNA polymerase transcription start.
CC   NM (pCKSP6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSP64)(pUC12)(PromSP6)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP64 remove SphI-BamHI 466bp
FT                   \ 2568..2999..35, Sp6 prom/MCS/2533bp
FT                   2. pSP64 SphI-BamHI 466bp 2568..2999..35, Sp6 prom/MCS
FT                   SphI-HphI 434bp 2568..2999..3, Sp6 prom/no MCS
FT                   :T4 DNA polymerase
FT                   -> pCKSP6 2966bp"
FT   misc_binding    complement(1..24)
FT                   /note="MCS BamHI-XbaI-SalI-PstI-HindIII
FT                   M13mp10/pUC12-polylinker, partial"
FT   misc_feature    complement(1..253)
FT                   /note="from pUC12"
FT   misc_feature    complement(254..2442)
FT                   /note="from pUC12"
FT   misc_feature    complement(2442..2721)
FT                   /note="from pBR322"
FT   misc_feature    2722..2966
FT                   /note="from bacteriophage Sp6"
FT   promoter        2948..2966
FT                   /note="PRO bacteriophage Sp6 promoter"
FT   misc_feature    1..1
FT                   /note="start of Sp6-RNA synthesis"
FT   CDS             1198..1986
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2966 BP; 735 A; 765 C; 751 G; 715 T; 0 other;
     ggatccccgg gcgagctcga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     ctatgcggca tcagagcaga ttgtactgag agtgcaccat atcgacgctc tcccttatgc
     gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt
     tccggggtgc ggaacggcgt taccagaaac tcagaaggtt cgtccaacca aaccgactct
     gacggcagtt tacgagagag atgatagggt ctgcttcagt aagccagatg ctacacaatt
     aggcttgtac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
     acacatacga tttaggtgac actata
//