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ID   PCL640     preliminary; circular DNA; SYN; 2121 BP.
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AC   IG9964;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCL640 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCL640 from pPolyIII-I
RC   plasmid from pCL640 & linker
RC   plasmid2 from plasmid & SV40 polyA
RC   pCL642 or pHMG from plasmid2 & mouse HMGCR promoter/exon/intron
RA   Gautier C., Mehtali M., Lathe R.F.;
RT   "A ubiquitous mammalian expression vector, pHMG, based on a
RT   housekeeping gene promoter";
RL   Nucleic Acids Res. 17:8389-8389(1989).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pCL640)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pPolyIII-I)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPolyIII-I BglII 2117bp 133..133, ori/amp
FT                   fill in
FT                   -> pCL640 2121bp"
FT   -               1..136
FT                   /note="pPolyIII-I 1..136 136bp
FT                   BglII = A^GATC T
FT                   BglII =      A^GATCT"
FT   -               137..2121
FT                   /note="pPolyIII-I 133..2117 1985bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2121 BP; 518 A; 554 C; 521 G; 528 T; 0 other;
     ggatctgcgg ccgccggcct cgagggccgg atccgaattc ccgggagagc tcgatatcgc
     atgcggtacc tctagaagaa gcttggccag ctggtcgacc tgcagatccg gccctcgagg
     ccggcggccg cagatcgatc tgcgacgcga ggctggatgg ccttccccat tatgattctt
     ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     gacgaccatc agggacagct tcacggccag caaaaggcca ggaaccgtaa aaaggccgcg
     ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgtgcag gcatcgtggt
     gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     gccctttcgt cttcaagaat t
//