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ID   PCM1       preliminary; circular DNA; SYN; 3982 BP.
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AC   IG0032; ATCC37176;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCM1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCM1, pCM4, pCM7 from pBR328 & pBR327
RC   [pCM3 from pBR328 & pBR327]
RC   pPV501, pPV502 from cat gene
RA   Close T.J., Rodriguez R.L.;
RT   "Construction and characterization of the chloramphenicol-resistance
RT   gene cartridge: a new approach to the transcriptional mapping of
RT   extrachromosomal elements";
RL   Gene 20:305-316(1982).
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CC   ATCC size is 3300 bp.
CC   Contains promoterless CAT gene flanked by SalI sites which can be
CC   used to construct other vectors. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCM1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli RR1)(E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR327)(Tn9)
CC   BR (pCM4)(pCM7)(pCM71)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Tn9 TaqI-TaqI 773bp 215..988, #V00622
FT                   SalI linker:SalI linker 709bp, cat gene
FT                   2. pBR327 SalI 3273bp 651..651
FT                   -> pCM1 3982bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             684..1344
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   from Tn9, inserted in SalI site"
FT   CDS             2984..3844
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3982 BP; 914 A; 1062 C; 1005 G; 1001 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggcccgt ggccggggga ctgttgggcg
     ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgacgagat tttcaggagc
     taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
     tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gtgctacgcc
     tgaataagtg ataataagcg gatgaatggc agaaattcgt cgaccgatgc ccttgagagc
     cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac
     ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     tccgccatct ccagcagccg cacgcggcgc atctcgggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     gaggcccttt cgtcttcaag aa
//