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ID   PCM4       preliminary; circular DNA; SYN; 3983 BP.
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AC   IG0033; ATCC37174;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCM4 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCM1, pCM4, pCM7 from pBR328 & pBR327
RC   [pCM3 from pBR328 & pBR327]
RC   pPV501, pPV502 from cat gene
RA   Close T.J., Rodriguez R.L.;
RT   "Construction and characterization of the chloramphenicol-resistance
RT   gene cartridge: a new approach to the transcriptional mapping of
RT   extrachromosomal elements";
RL   Gene 20:305-316(1982).
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CC   ATCC size is 4200 bp.
CC   Contains promoterless CAT gene flanked by BamHI sites which can be
CC   used to construct other vectors. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCM4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli RR1)(E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR327)(Tn9)
CC   BR (pCM1)(pCM7)(pCM71)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Tn9 TaqI-TaqI 773bp 215..988, #V00622
FT                   SalI linker:SalI linker 710bp, cat gene
FT                   2. pBR327 SalI 3273bp 652..652
FT                   -> pCM4 3983bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             409..1069
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   from Tn9, inserted in SalI site"
FT   CDS             2985..3845
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3983 BP; 914 A; 1062 C; 1006 G; 1001 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     gcccttaaac gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa
     ttcgggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggcccgt ggccggggga ctgttgggcg
     ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag
     ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca
     cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     gtccgccatc tccagcagcc gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc
     ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     cgaggccctt tcgtcttcaa gaa
//