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ID   PCM7       preliminary; circular DNA; SYN; 4060 BP.
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AC   IG0034; ATCC37173;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCM7 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCM1, pCM4, pCM7 from pBR328 & pBR327
RC   [pCM3 from pBR328 & pBR327]
RC   pPV501, pPV502 from cat gene
RA   Close T.J., Rodriguez R.L.;
RT   "Construction and characterization of the chloramphenicol-resistance
RT   gene cartridge: a new approach to the transcriptional mapping of
RT   extrachromosomal elements";
RL   Gene 20:305-316(1982).
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RN   [2]
RC   plasmid from pBR325 & yeast 2 micron
RC   pYT11-LEU2 from plasmid & pYeleu10, yeast LEU2 gene
RA   Cohen J.D., Eccleshall T.R., Needleman R.B., Federoff H.,
RA   Buchferer B.A., Marmur J.;
RT   "Functional expression in yeast of the Escherichia coli plasmid
RT   gene coding for chloramphenicol acetyltransferase";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:1078-1082(1980).
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RN   [3]
RC   JR66 from cat gene, CAT I
RC   s-a from cat gene, CAT II
RC   R387 from cat gene, CAT III
RA   Zaidenzaig Y., Shaw W.V.;
RT   "Affinity and hydrophobic chromatography of three variants of
RT   chloramphenicol acetyltransferases specified by R factors in
RT   Escherichia coli";
RL   FEBS Lett. 62:266-271(1976).
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CC   ATCC size is 4200 bp.
CC   CAT is expressed in both prokaryotes and eukaryotes.
CC   Contains promoterless CAT gene flanked by HindIII sites which can
CC   be used to construct other vectors. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCM7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)(ATCC)
CC   HO (E.coli RR1)(E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR327)(Tn9)
CC   BR (pCM1)(pCM4)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Tn9 TaqI-TaqI 773bp 215..988, #V00622
FT                   HindIII linker:HindIII linker 787bp, cat gene
FT                   2. pBR327 HindIII 3273bp 30..30
FT                   -> pCM7 4060bp"
FT   CDS             29..816
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   promoterless and terminatorless,
FT                   from Tn9 HindIII-HindIII fragment"
FT   RBS             >29..<280
FT                   /note="RBS E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_feature    >29..<280
FT                   /note="ATG E. coli CAT gene start codon"
FT   misc_binding    581..581
FT                   /note="SIT NcoI"
FT   misc_feature    >581..<816
FT                   /note="TAA"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 4060 BP; 935 A; 1078 C; 1018 G; 1029 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttcgacga gattttcagg agctaaggaa
     gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atcgcatcgt
     aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa
     gtgataataa gcggatgaat ggcagaaatt cgtcgaagct ttaatgcggt agtttatcac
     agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
     gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggcccgtggc
     cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
     tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggccgc
     gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//