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ID   PCM71      preliminary; circular DNA; SYN; 3370 BP.
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AC   IG0035;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCM71 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBR350 from pBR322
RC   pEB1 from pBR328 & pUB110
RC   pCM71 from pCM1 & pCM7
RC   pGR1 from pUB110 & pBR350
RC   pGR71 from pGR1 & pCM71
RC   pGR71- series from pGR71
RA   Goldfarb D.S., Doi R.H., Rodriguez R.L.;
RT   "Expression of Tn9-derived chloramphenicol resistance in Bacillus
RT   subtilis";
RL   Nature 293:309-311(1981).
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CC   pCM71 is a smaller vector than pCM1 or pCM7 and is used in
CC   constructing pGR71, which has a HindIII site directly in front
CC   of the CAT gene.
CC   NM (pCM71)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR327)(Tn9)
CC   BR (pCM1)(pCM4)(pCM7)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCM1 EcoRI-NruI 3127bp 1683..3982..828,
FT                   \ ori/amp/cat
FT                   2. pCM7 HindIII-EcoRI 251bp 30..281, cat gene
FT                   \ pCM7 HindIII = 30 817
FT                   -> pCM71 3370bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             9..454
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   from Tn9, inserted in SalI site"
FT   CDS             2094..2954
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 3370 BP; 813 A; 874 C; 821 G; 862 T; 0 other;
     ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     gtgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcgt cgaccgatgc
     ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggcc gcgttgctgg
     cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     ggcgtatcac gaggcccttt cgaattcttc aagtttgaca gcttatcatc gataagcttc
     gacgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     aaataagcac aagttttatc cggcctttga attcacattc ttgcccgcct gatgaatgct
     catccggaaa
//