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ID   PCMVEBNA   preliminary; circular DNA; SYN; 5452 BP.
XX
AC   IG1059;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCMVEBNA - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2686
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RC   pUC18 from pUC8
RC   pUC19 from pUC9
RA   Yanisch-Perron C., Vieira J., Messing J.;
RT   "Improved M13 phage cloning vectors and host strains: nucleotide
RT   sequences of the M13mp18 and pUC19 vectors";
RL   Gene 33:103-119(1985).
XX
CC   CLONTECH sequence is given as the inverse complement of this sequence
CC   and starts at base 2295 of this sequence.
CC   NM (pCMVEBNA)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)(Invitrogen)
CC   HO (E.coli)(human fibroblast or lymphoblast cells)
CC   CP ()
CC   FN (helper)
CC   SE ()
CC   PA (EBV)(CMV)(pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. CMV, immediate early promoter
FT                   3. EBV, EBNA-1 gene
FT                   4. E. coli, lacI/lacZ genes
FT                   -> pCMVEBNA 5452bp"
FT   misc_binding    0..0
FT                   /note="SIT HgiEII"
FT   misc_binding    0..0
FT                   /note="SIT NarI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        0..0
FT                   /note="PRO human cytomegalovirus (CMV)
FT                   immediate early gene"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   misc_binding    0..0
FT                   /note="SIT BglI"
FT   misc_binding    0..0
FT                   /note="SIT FspI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT NcoI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SphI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   misc_binding    0..0
FT                   /note="SIT AflIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT Cfr101"
FT   misc_binding    0..0
FT                   /note="SIT GsuI"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT AvaII"
FT   misc_binding    0..0
FT                   /note="SIT FspI"
FT   misc_binding    0..0
FT                   /note="SIT AvaII"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT ScaI"
FT   misc_binding    0..0
FT                   /note="SIT XmnI"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
FT   misc_binding    0..0
FT                   /note="SIT AatII"
FT   misc_binding    0..0
FT                   /note="SIT DraII"
XX
SQ   Sequence 5452 BP; 1108 A; 1736 C; 1246 G; 1362 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtct
     actggggatt tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc
     tcctaacaag ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc
     tccgtcatct ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg
     aggcaaatct actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc
     gggctttgtc ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt
     ttgtaaaaag accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc
     gggtccaggg gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat
     agcaagggca gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa
     cacaccggcg acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag
     agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg
     ctttccaaac caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt
     gcttgggcct tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc
     aggctcacca tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg
     gtggaaaaat ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga
     ctgactactg ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct
     ttcacgactt ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc
     tcgaccccgg cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc
     tccacctcct gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc
     tgcccctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc
     tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
     tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     tcctgctcct gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     tcctcctgct cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc
     accgtgggtc cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc
     tctatgtctt ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc
     tcgtcctctt ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc
     gccggagcct tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt
     acctggcccc tcgtcagaca tgattcacac taaaagagat ccccgggtac ccggggatcc
     tctagagtca ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg
     cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt
     acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     gtcaataatc aatgtcaaca tggcggtaat gttggacatg agccaatata aatgtacata
     ttatgatatg gatacaacgt atgcaatggg ccaagcttgg cgtaatcatg gtcatagctg
     tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//