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ID   PCOR112N   preliminary; circular DNA; SYN; 4412 BP.
XX
AC   Z29587;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCOR112N - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4412
RC   pCOR112N from pCOR116
RC   pCOR114N from pCOR116
RC   pCOR116N from pCOR116
RA   Liu L., Dasgupta I., Davies J.W., Hull R.;
RT   "Modified vectors for monocot transformation toward virus resistance";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4412
RC   pAct-A from rice, act1
RC   pCOR101, pCOR102 from pBluescript II KS & pAct-A
RC   pCOR103 from pBluescript II KS & pCOR101
RC   pCOR106 from pGEM-5Zf+ & pCOR101
RC   pCOR107 from pCOR106
RC   pCOR104 from pCOR101
RC   pCOR105 from pCOR104
RC   pCOR108 from pCOR102
RC   pCOR109 from pCOR102 & pGEM-5Zf+
RC   pCOR110, pCOR111 from pCOR109 & pBluescript II KS
RC   pCOR112 from pCOR111
RC   pCOR113 from pCOR111
RC   pCOR114 from pCOR110
RC   pCOR115, pCOR116 from pCOR109
RC   pCOR117 from pCOR116
RC   pAct1-F2 from pAct1-F & pCOR103
RC   pAct1-F3 from pAct1-F2
RC   pAct1-F4 from pAct1-F2 & pRAJ275
RC   pAct1-F5 from pAct1-F2 & pRAJ275
RC   pAct1-G72 from pAct1-F & pSP72
RC   pAct1-F6 from pAct1-G72
RC   pAct1-I72 from pAct1-F2 & pSP72
RC   pAct1-F7 from pAct1-I72 & pAct1-F2
RC   pAct1-R1 from pAct1-I72 & pAct1-R
RC   pBCG from pBluescript II KS & pBI221.1
RC   pBCG-A from pBCG & pAct1-F6
RC   pBCG-A4 from pBCG-A & pAct1-F4
RC   pBCG-A5 from pBCG-A & pAct1-F5
RC   pBCG-4I from pBCG & pAct1-F6
RC   pCaMVI1CN from CaMV
RC   pCI1GusN from pAI1GusN & pCaMVI1CN
RA   McElroy D., Blowers A.D., Jenes B., Wu R.;
RT   "Construction of expression vectors based on the rice actin 1 (Act1)
RT   5' region for use in monocot transformation";
RL   Mol. Gen. Genet. 231:150-160(1991).
XX
RN   [3]
RP   27-282
RC   pT37H23 from pBR322 & pTiT37, NOS gene
RA   Depicker A., Stachel S., Dhaese P., Zambryski P., Goodman H.M.;
RT   "Nopaline synthase: transcript and DNA sequence";
RL   J. Mol. Appl. Genet. 1:561-573(1982).
XX
RN   [4]
RP   1-4412
RC   pCOR112N
RA   Liu L.;
RT   ;
RL   Submitted (26-JAN-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Liu L., John Innes Institute, Virus Research, Colney Lane, Norwich,
RL   NR4 7UH, United Kingdom.
XX
CC   NCBI gi: 452344
CC   NM (pCOR112N)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pCOR112)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. rice 2200bp, Act1 5' region
FT                   -> pAct-A
FT                   1. pBluescript II KS+ EcoRV 2961bp 716..716, MCS
FT                   2. pAct-A EcoRV-EcoRV 2200bp, Act1 5' region
FT                   mutagenesis makes NcoI at ATG
FT                   -> pCOR102 5200bp
FT                   1. pCOR102 EagI-NcoI 2200bp, Act1 5' region
FT                   2. pGEM-5Zf+ NcoI-EagI 2997bp 38..3003..32, MCS
FT                   -> pCOR109 5200bp
FT                   1. pCOR109 XhoI-SalI 1400bp, Act1 5' region
FT                   2. pBluescript II KS+ XhoI 2961bp 741..741, MCS
FT                   -> pCOR111 4400bp
FT                   1. pCOR111 remove small EcoRV-EcoRV, MCS/4400bp
FT                   -> pCOR112 4412bp"
FT   misc_binding    1..30
FT                   /note="MCS"
FT   misc_feature    3..5
FT                   /note="start codon for gene expression"
FT   terminator      27..282
FT                   /note="plasmid Ti nos gene terminator [3]"
FT   rep_origin      506..962
FT                   /note="ORI phage f1 region"
FT   CDS             1093..1953
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   rep_origin      1954..2894
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        3181..4412
FT                   /note="PRO rice actin 1 5' region (act1 promoter) [2]"
XX
SQ   Sequence 4412 BP; 1127 A; 1107 C; 1036 G; 1142 T; 0 other;
     ccatggccgc gggatatcga attcctgcag atcgttcaaa catttggcaa taaagtttct
     taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tctctagagc ggccgccacc
     gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac cgggcccccc
     ctcgaggtca ttcatatgct tgagaagaga gtcgggatag tccaaaataa aacaaaggta
     agattacctg gtcaaaagtg aaaacatcag ttaaaaggtg gtataagtaa aatatcggta
     ataaaaggtg gcccaaagtg aaatttactc ttttctacta ttataaaaat tgaggatgtt
     ttgtcggtac tttgatacgt catttttgta tgaattggtt tttaagttta ttcgcgattt
     ggaaatgcat atctgtattt gagtcggttt ttaagttcgt tgcttttgta aatacagagg
     gatttgtata agaaatatct ttaaaaaacc catatgctaa tttgacataa tttttgagaa
     aaatatatat tcaggcgaat tccacaatga acaataataa gattaaaata gcttgccccc
     gttgcagcga tgggtatttt ttctagtaaa ataaaagata aacttagact caaaacattt
     acaaaaacaa cccctaaagt cctaaagccc aaagtgctat gcacgatcca tagcaagccc
     agcccaaccc aacccaaccc aacccacccc agtgcagcca actggcaaat agtctccacc
     cccggcacta tcaccgtgag ttgtccgcac caccgcacgt ctcgcagcca aaaaaaaaaa
     aagaaagaaa aaaaagaaaa agaaaaacag caggtgggtc cgggtcgtgg gggccggaaa
     agcgaggagg atcgcgagca gcgacgaggc ccggccctcc ctccgcttcc aaagaaacgc
     cccccatcgc cactatatac ataccccccc ctctcctccc atccccccaa ccctaccacc
     accaccacca ccacctcctc ccccctcgct gccggacgac gagctcctcc cccctccccc
     tccgccgccg ccggtaacca ccccgcccct ctcctctttc tttctccgtt ttttttttcg
     tctcggtctc gatctttggc cttggtagtt tgggtgggcg agagcggctt cgtcgcccag
     atcggtgcgc gggaggggcg ggatctcgcg gctggcgtct ccgggcgtga gtcggcccgg
     atcctcgcgg ggaatggggc tctcggatgt agatcttctt tctttcttct ttttgtggta
     gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat
     gcagcctcgt gcggagcttt tttgtaggta ga
//