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ID   PCOR114N   preliminary; circular DNA; SYN; 4433 BP.
XX
AC   Z29588;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCOR114N - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4433
RC   pCOR112N from pCOR116
RC   pCOR114N from pCOR116
RC   pCOR116N from pCOR116
RA   Liu L., Dasgupta I., Davies J.W., Hull R.;
RT   "Modified vectors for monocot transformation toward virus resistance";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4433
RC   pAct-A from rice, act1
RC   pCOR101, pCOR102 from pBluescript II KS & pAct-A
RC   pCOR103 from pBluescript II KS & pCOR101
RC   pCOR106 from pGEM-5Zf+ & pCOR101
RC   pCOR107 from pCOR106
RC   pCOR104 from pCOR101
RC   pCOR105 from pCOR104
RC   pCOR108 from pCOR102
RC   pCOR109 from pCOR102 & pGEM-5Zf+
RC   pCOR110, pCOR111 from pCOR109 & pBluescript II KS
RC   pCOR112 from pCOR111
RC   pCOR113 from pCOR111
RC   pCOR114 from pCOR110
RC   pCOR115, pCOR116 from pCOR109
RC   pCOR117 from pCOR116
RC   pAct1-F2 from pAct1-F & pCOR103
RC   pAct1-F3 from pAct1-F2
RC   pAct1-F4 from pAct1-F2 & pRAJ275
RC   pAct1-F5 from pAct1-F2 & pRAJ275
RC   pAct1-G72 from pAct1-F & pSP72
RC   pAct1-F6 from pAct1-G72
RC   pAct1-I72 from pAct1-F2 & pSP72
RC   pAct1-F7 from pAct1-I72 & pAct1-F2
RC   pAct1-R1 from pAct1-I72 & pAct1-R
RC   pBCG from pBluescript II KS & pBI221.1
RC   pBCG-A from pBCG & pAct1-F6
RC   pBCG-A4 from pBCG-A & pAct1-F4
RC   pBCG-A5 from pBCG-A & pAct1-F5
RC   pBCG-4I from pBCG & pAct1-F6
RC   pCaMVI1CN from CaMV
RC   pCI1GusN from pAI1GusN & pCaMVI1CN
RA   McElroy D., Blowers A.D., Jenes B., Wu R.;
RT   "Construction of expression vectors based on the rice actin 1 (Act1)
RT   5' region for use in monocot transformation";
RL   Mol. Gen. Genet. 231:150-160(1991).
XX
RN   [3]
RP   1-4433
RC   pCOR114N
RA   Liu L.;
RT   ;
RL   Submitted (26-JAN-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Liu L., John Innes Institute, Virus Research, Colney Lane, Norwich,
RL   NR4 7UH, United Kingdom.
XX
CC   NCBI gi: 452346
CC   NM (pCOR114N)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pCOR114)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. rice 2200bp, Act1 5' region
FT                   -> pAct-A
FT                   1. pBluescript II KS EcoRV 2961bp 716..716, MCS
FT                   2. pAct-A EcoRV-EcoRV 2200bp, Act1 5' region
FT                   mutagenesis makes NcoI at ATG
FT                   -> pCOR102 5200bp
FT                   1. pCOR102 EagI-NcoI 2200bp, Act1 5' region
FT                   2. pGEM-5Zf+ NcoI-EagI 2997bp 38..3003..32, MCS
FT                   -> pCOR109 5200bp
FT                   1. pCOR109 XhoI-SalI 1400bp, Act1 5' region
FT                   2. pBluescript II KS XhoI 2961bp 741..741, MCS
FT                   -> pCOR110 4400bp
FT                   1. pCOR110 remove small HindIII-EagI, 4400bp
FT                   exonuclease III
FT                   mung bean nuclease
FT                   -> pCOR114 4433bp"
FT   misc_binding    1..38
FT                   /note="MCS"
FT   misc_feature    3..5
FT                   /note="start codon for gene expression"
FT   terminator      35..290
FT                   /note="plasmid Ti nos gene terminator [3]"
FT   rep_origin      586..1526
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1527..2387)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   rep_origin      2518..2974
FT                   /note="ORI phage f1 region"
FT   promoter        3202..4433
FT                   /note="PRO rice actin 1 5' region (act1 promoter) [2]"
XX
SQ   Sequence 4433 BP; 1157 A; 1096 C; 1058 G; 1122 T; 0 other;
     ccatggccgc gggatatcac tagtgcggcc gcctgcagat cgttcaaaca tttggcaata
     aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc tctagagtcg
     acgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctccaccgcg
     gtggctatcg ataccgtcga cctcgaggtc attcatatgc ttgagaagag agtcgggata
     gtccaaaata aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt
     ggtataagta aaatatcggt aataaaaggt ggcccaaagt gaaatttact cttttctact
     attataaaaa ttgaggatgt tttgtcggta ctttgatacg tcatttttgt atgaattggt
     ttttaagttt attcgcgatt tggaaatgca tatctgtatt tgagtcggtt tttaagttcg
     ttgcttttgt aaatacagag ggatttgtat aagaaatatc tttaaaaaac ccatatgcta
     atttgacata atttttgaga aaaatatata ttcaggcgaa ttccacaatg aacaataata
     agattaaaat agcttgcccc cgttgcagcg atgggtattt tttctagtaa aataaaagat
     aaacttagac tcaaaacatt tacaaaaaca acccctaaag tcctaaagcc caaagtgcta
     tgcacgatcc atagcaagcc cagcccaacc caacccaacc caacccaccc cagtgcagcc
     aactggcaaa tagtctccac ccccggcact atcaccgtga gttgtccgca ccaccgcacg
     tctcgcagcc aaaaaaaaaa aaagaaagaa aaaaaagaaa aagaaaaaca gcaggtgggt
     ccgggtcgtg ggggccggaa aagcgaggag gatcgcgagc agcgacgagg cccggccctc
     cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc
     catcccccca accctaccac caccaccacc accacctcct cccccctcgc tgccggacga
     cgagctcctc ccccctcccc ctccgccgcc gccggtaacc accccgcccc tctcctcttt
     ctttctccgt tttttttttc gtctcggtct cgatctttgg ccttggtagt ttgggtgggc
     gagagcggct tcgtcgccca gatcggtgcg cgggaggggc gggatctcgc ggctggcgtc
     tccgggcgtg agtcggcccg gatcctcgcg gggaatgggg ctctcggatg tagatcttct
     ttctttcttc tttttgtggt agaatttgaa tccctcagca ttgttcatcg gtagtttttc
     ttttcatgat ttgtgacaaa tgcagcctcg tgcggagctt ttttgtaggt aga
//