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ID   PCOR116N   preliminary; circular DNA; SYN; 4486 BP.
XX
AC   Z29589;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCOR116N - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4486
RC   pCOR112N from pCOR116
RC   pCOR114N from pCOR116
RC   pCOR116N from pCOR116
RA   Liu L., Dasgupta I., Davies J.W., Hull R.;
RT   "Modified vectors for monocot transformation toward virus resistance";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4486
RC   pAct-A from rice, act1
RC   pCOR101, pCOR102 from pBluescript II KS & pAct-A
RC   pCOR103 from pBluescript II KS & pCOR101
RC   pCOR106 from pGEM-5Zf+ & pCOR101
RC   pCOR107 from pCOR106
RC   pCOR104 from pCOR101
RC   pCOR105 from pCOR104
RC   pCOR108 from pCOR102
RC   pCOR109 from pCOR102 & pGEM-5Zf+
RC   pCOR110, pCOR111 from pCOR109 & pBluescript II KS
RC   pCOR112 from pCOR111
RC   pCOR113 from pCOR111
RC   pCOR114 from pCOR110
RC   pCOR115, pCOR116 from pCOR109
RC   pCOR117 from pCOR116
RC   pAct1-F2 from pAct1-F & pCOR103
RC   pAct1-F3 from pAct1-F2
RC   pAct1-F4 from pAct1-F2 & pRAJ275
RC   pAct1-F5 from pAct1-F2 & pRAJ275
RC   pAct1-G72 from pAct1-F & pSP72
RC   pAct1-F6 from pAct1-G72
RC   pAct1-I72 from pAct1-F2 & pSP72
RC   pAct1-F7 from pAct1-I72 & pAct1-F2
RC   pAct1-R1 from pAct1-I72 & pAct1-R
RC   pBCG from pBluescript II KS & pBI221.1
RC   pBCG-A from pBCG & pAct1-F6
RC   pBCG-A4 from pBCG-A & pAct1-F4
RC   pBCG-A5 from pBCG-A & pAct1-F5
RC   pBCG-4I from pBCG & pAct1-F6
RC   pCaMVI1CN from CaMV
RC   pCI1GusN from pAI1GusN & pCaMVI1CN
RA   McElroy D., Blowers A.D., Jenes B., Wu R.;
RT   "Construction of expression vectors based on the rice actin 1 (Act1)
RT   5' region for use in monocot transformation";
RL   Mol. Gen. Genet. 231:150-160(1991).
XX
RN   [3]
RP   1-4486
RC   pCOR116N
RA   Liu L.;
RT   ;
RL   Submitted (26-JAN-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Liu L., John Innes Institute, Virus Research, Colney Lane, Norwich,
RL   NR4 7UH, United Kingdom.
XX
CC   NCBI gi: 452348
CC   NM (pCOR116N)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pCOR116)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. rice 2200bp, Act1 5' region
FT                   -> pAct-A
FT                   1. pBluescript II KS EcoRV 2961bp 716..716, MCS
FT                   2. pAct-A EcoRV-EcoRV 2200bp, Act1 5' region
FT                   mutagenesis makes NcoI at ATG
FT                   -> pCOR102 5200bp
FT                   1. pCOR102 EagI-NcoI 2200bp, Act1 5' region
FT                   2. pGEM-5Zf+ NcoI-EagI 2997bp 38..3003..32, MCS
FT                   -> pCOR109 5200bp
FT                   1. pCOR109 remove NheI-SpeI 1400bp, Act1 5' region
FT                   2. pCOR109 SpeI-SpeI 3000bp, pBluescript II KS+ 684
FT                   \ pGEM-5Zf+ 56
FT                   -> pCOR116 4486bp"
FT   misc_binding    1..52
FT                   /note="MCS"
FT   misc_feature    3..5
FT                   /note="start codon for gene expression"
FT   terminator      49..304
FT                   /note="plasmid Ti nos gene terminator [3]"
FT   rep_origin      511..967
FT                   /note="ORI phage f1 region"
FT   CDS             1153..2013
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   rep_origin      2014..2954
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        3256..4486
FT                   /note="PRO rice actin 1 5' region (act1 promoter) [2]"
XX
SQ   Sequence 4486 BP; 1143 A; 1118 C; 1048 G; 1177 T; 0 other;
     ccatggccgc gggatatcac tagttctaga gcggccggga gcatgcctgc agatcgttca
     aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     gatctctaga gtcgacgggc ccaattcgcc ctatagtgag tcgtattaca attcactggc
     cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
     gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     aatcgggggc tccctttagg gttccgattt agagctttac ggcacctcga ccgcaaaaaa
     cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     ttaaaaaatg agctgattta acaaatattt aacgcgaatt ttaacaaaat attaacgttt
     acaatttcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     acaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtcat
     acactattat cccgtattga cgccgggcaa gagcaactcg gtcgccgggc gcggtattct
     cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     gacaccacga tgcctgtagc aatgccaaca acgttgcgca aactattaac tggcgaacta
     cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     agcctatcga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg
     agctctccca tatggtcgag gtcattcata tgcttgagaa gagagtcggg atagtccaaa
     ataaaacaaa ggtaagatta cctggtcaaa agtgaaaaca tcagttaaaa ggtggtataa
     gtaaaatatc ggtaataaaa ggtggcccaa agtgaaattt actcttttct actattataa
     aaattgagga tgttttgtcg gtactttgat acgtcatttt tgtatgaatt ggtttttaag
     tttattcgcg atttggaaat gcatatctgt atttgagtcg gtttttaagt tcgttgcttt
     tgtaaataca gagggatttg tataagaaat atctttaaaa aacccatatg ctaatttgac
     ataatttttg agaaaaatat atattcaggc gaattccaca atgaacaata ataagattaa
     aatagcttgc ccccgttgca gcgatgggta ttttttctag taaaataaaa gataaactta
     gactcaaaac atttacaaaa acaaccccta aagtcctaaa gcccaaagtg ctatgcacga
     tccatagcaa gcccagccca acccaaccca acccaaccca ccccagtgca gccaactggc
     aaatagtctc cacccccggc actatcaccg tgagttgtcc gcaccaccgc acgtctcgca
     gccaaaaaaa aaaaaagaaa gaaaaaaaag aaaaagaaaa acagcaggtg ggtccgggtc
     gtgggggccg gaaaagcgag gaggatcgcg agcagcgacg aggcccggcc ctccctccgc
     ttccaaagaa acgcccccca tcgccactat atacataccc ccccctctcc tcccatcccc
     ccaaccctac caccaccacc accaccacct cctcccccct cgctgccgga cgacgagctc
     ctcccccctc cccctccgcc gccgccggta accaccccgc ccctctcctc tttctttctc
     cgtttttttt ttcgtctcgg tctcgatctt tggccttggt agtttgggtg ggcgagagcg
     gcttcgtcgc ccagatcggt gcgcgggagg ggcgggatct cgcggctggc gtctccgggc
     gtgagtcggc ccggatcctc gcggggaatg gggctctcgg atgtagatct tctttctttc
     ttctttttgt ggtagaattt gaatccctca gcattgttca tcggtagttt ttcttttcat
     gatttgtgac aaatgcagcc tcgtgcggag cttttttgta ggtaga
//