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ID   PCOS5      preliminary; circular DNA; SYN; 7286 BP.
XX
AC   U13206;
XX
DT   14-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli vector pCOS5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7286
RC   pCOS5 from lambda, cos
RA   Connell T.D., Martone A.J., Holmes R.K.;
RT   "A new mobilizable cosmid vector for use in Vibrio cholerae and
RT   other gram-negative bacteria";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-7286
RC   pCOS5
RA   Connell T.D.;
RT   ;
RL   Submitted (09-AUG-1994) by:
RL   Connell T.D., University at Buffalo, Department of Microbiology,
RL   3435 Main Street, Buffalo, NY 14214, USA.
XX
CC   NCBI gi: 537613
CC   NM (pCOS5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR325 5996bp, ori/amp gene/cat gene
FT                   2. lambda 1300bp, cos
FT                   -> pCOS5 7286bp"
FT   CDS             complement(528..1187)
FT                   /note="ANT E. coli chloramphenicol resistance gene
FT                   (cat)"
FT   CDS             complement(6218..7078)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
XX
SQ   Sequence 7286 BP; 1792 A; 1945 C; 1756 G; 1793 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttatcggc cagcctcgca ggcaggattc
     ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatt
     cgctataatg accccgaagc agggttatgc agcggaaaag atccgtcgac ctgcagccaa
     ggacggatcc tctagagtcg gcatttgaga agcacacggt cacactgctt ccggtagtca
     ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
     cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca
     ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
     tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggacctg
     caggcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa
     cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat
     ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
     ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
     ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc
     atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc
     atgatctgcc acattacgct cctgtccggc aaagttacct ctgccgaagt tgagtatttt
     tgctgtattt gtcataatga ctcctgttga tagatccagt aatgacctca gaactccatc
     tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatcgcagca
     acttgtcgcg ccaatcgagc catgtcgtcg tcaacgaccc cccattcaag aacagcaagc
     agcattgaga actttggaat ccagtccctc ttccacctgc tgatctgcga cttatcaacg
     ccgacagctt ccgctgtctt ctcagttcca agcattgcga ttttgttaag caacgcactc
     tcgattcgta gagcctcgtt gcgtttgttt gcacgaacca tatgtaagta tttccttaga
     taacaattga ttgaatgtat gcaaataaat gcatacacca taggtgtggt ttaatttgat
     gccctttttc agggctggaa tgtgtaagag cgggttattt atgctgttgt ttttttgtta
     ctcgggaagg gctttacctc ttccgcataa acgcttccat cagcgtttat agttaaaaaa
     atctttcggc ctgcatgaat ggccttgttg atcgcgattt gatatacgcc gagatctaac
     gggcatcagt aaagtccagc tcctgctggc ggatgacgca tgcattatgc tcgcagagat
     aaaacacgct ggaggggtca tccggcgtcc atttgaggcc aaacggcgtc tctttgtcgc
     caaatttaag atactgctcc tccccgcaat gcgggcaggc aacatgaaaa cgcataaaat
     gcggggattc actggctgca cgctcaatct gacaggtgcc tctcactttt ggcgtggagc
     cacggatgga ctttggccag accgagcctt caatacgctt gtcacccagg aacgtcggag
     agccttcctg ttcaatatca tcatcaaaag cagcaagttc atcataaccc gccacatcca
     ccgacttttc acggtagttt tttgccgctt taccgcccag gcaccagaag ccacgcccat
     tagtgaaacg cttcatggtg agcgtgttaa cccggtgctt tttgccatac cacggggcca
     gcgccagcag cgacggaata tcacgaatag tcggctcaac gtgggttttc ataaagttct
     cggcatcacc atccgtcggc aaccagataa gggtgttgcg ctgcttatgc tctataaagt
     aggcataaac acccagcagc attttggaat aaccgacacg ggcagacttc accacattca
     cctcacggat gtagtcgctg cccatcgcat tcatgatggc ccgctgaaag ggcagtgttt
     cccagcgccc ttcctggtat gcggattctt tcgggagata gtaattagca tccgcccatt
     caacggcggt ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc
     gcagcctgtt atcctgactg ttcgatatat tcactcagca accccggtat cagttcatcc
     agcgcggctg ctttgttcat ggctttgatg atatcccgtt tcaggaaatc aacatgtcgg
     ttttccagtt ccggaaaacg ccgctgcacc gacaggggga gcccgtcgag aatactggca
     atttcacctg cgatccgcga cagcacgaaa gtacagaatg cggttaccac cacttcagcg
     gagtctctgg cattcttcag ttcctgtgcg tcggcctgcg cacgcgtaag tcgatggcgt
     tcgtactcaa tagttcctgg ctggggatct gcctcgctgg cctgccgcag ttcttcaacc
     tcccggcgca gcttttcgtt ctcaatttca gcatcccttt cggcatacca ttttatgacg
     gcggcagagt cataaagcac ctcattaccc ttgccaccgc ctcgcagaac gggcattccc
     tgttcctgcc agttctgaat ggtacggata ctcgcaccga aaatgtcagc cagctgcttt
     ttgttgactt ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca
     aaaagcctcg ctttcagcac ctgtcgtttc ctttcttttc agagggtatt ttaaataaaa
     acattaagtt atgacgaaga agaacggaaa cgccttaaac cggaaaattt tcataaatag
     cgaaaacccg cgaggtcgcc gccccgtaac ctgtcggatc accggaaagg acccgtaaag
     tgataatgat tatcatctac atatcacaac gtgcgtggag gccatcaaac cacgtcaaat
     aatcaattat gacgcaggta tcgtattaat agatctgcat cgcaggatgc tgctggctac
     cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg
     catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta
     cccccatgaa cagaaattcc cccttacacg gaggcatcaa gtgaccaaac aggaaaaaac
     cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa
     cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga
     gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     tccgcttcct cgctcactga ctcgctgctc tcggtcgttc ggctgcggcg agcggtatca
     gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaa
//