back Return to this vector's summary.
ID   PCR1000    preliminary; circular DNA; SYN; 2922 BP.
XX
AC   IG1060;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pCR1000 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
XX
CC   NM (pCR1000)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning cDNA)(deletions nested)
CC   SE ()
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19
FT                   -> pCR1000 2922bp"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS ApaI-XbaI-NsiI-SphI-XhoI-XmaIII-NotI-
FT                   BstXI-EcoRV-PstI-EcoRI-BstXI-XmaIII-SpeI-BamHI-
FT                   SacI-KpnI-HindIII-NsiI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    511..511
FT                   /note="SIT AflIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1910..1910
FT                   /note="SIT SacII"
FT   misc_binding    1999..1999
FT                   /note="SIT XmnI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 2922 BP; 677 A; 848 C; 765 G; 632 T; 0 other;
     tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctact gaacgtgatc cccaccggaa ttgcggccgc ccaatacgca aaccgcctct
     ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     aggaaacagc tatgaccatg attacgccaa gctcaatacg actcactata gggcccggta
     ccgagctcac tagtttaatt aaaagcttat cggccgaggt gagaagggtt tcgatatcga
     gagaaacggt tctcaccgcg gccgcgaatt cactggccgt cgttttacaa cgtcgtgact
     gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     gcgaatggcg gccgcccggg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     caatatcacg ggtatccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     agtcgttgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     catggatcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     gagcaaggcg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
     caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc
     ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
     gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata
     aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg
     cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     gtttctgcgg actggctttc tacgtgttcc ggttccttta gcagcccttg cgccctgagt
     gcttgcggca gcgtgaagct aattcatggt taaatttttg ttaaatcagc tcatttttta
     accaataggc cgaaatcgca aaaatccctt aataaatcaa aagaatagcc cgagataggg
     ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     aaagggcgaa aaaccgtcta tcagggcgat ggcggatcag ct
//