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ID   PCRII      preliminary; circular DNA; SYN; 3932 BP.
XX
AC   IG1150;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pCRII - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
XX
CC   NM (pCRII)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning cDNA)(deletions nested)
CC   SE ()
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19
FT                   -> pCRII 3932bp"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS ApaI-XbaI-NsiI-SphI-XhoI-XmaIII-NotI-
FT                   BstXI-EcoRV-PstI-EcoRI-BstXI-XmaIII-SpeI-BamHI-
FT                   SacI-KpnI-HindIII-NsiI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    511..511
FT                   /note="SIT AflIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1910..1910
FT                   /note="SIT SacII"
FT   misc_binding    1999..1999
FT                   /note="SIT XmnI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 3932 BP; 916 A; 1011 C; 1064 G; 941 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcga ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
     ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
     ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc
     gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     aatcgggggc tccctttagg gttccgattt agagctttac ggcacctcga ccgcaaaaaa
     cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     aaccctatcg cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     ttaaaaaatg agctgattta acaaattcag ggcgcaaggg ctgctaaagg aaccggaaca
     cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
     tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     atctcctgtc atctcgcctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     gcgaggatct cgtcgtgatc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     gccgcttttc tggattcaac gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     tagcgttgga tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     acgagttctt ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     taaagttctg ctatgtgata cactattatc ccgtattgac gccgggcaag agcaactcgg
     tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     cataccaaac gacgagagtg acaccacgat gcctgtagca atgccaacaa cgttgcgcaa
     actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actgaatgga
     ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     tggtaagcgc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     cctgggcttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     gcgcagcgag tcagtgagcg aggaagcgga ag
//