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ID   PCS19      preliminary; circular DNA; SYN; 9344 BP.
XX
AC   M98046; ATCC77409;
XX
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pCS19 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCS19 from pBR322 & URA3 gene & trp terminator & CEN15 & cI gene/pR
RA   Sengstag C.G., Hinnen A.;
RT   "The sequence of the Saccharomyces cerevisiae gene PHO2 codes for
RT   a regulatory protein with unusual aminoacid composition";
RL   Nucleic Acids Res. 15:233-246(1987).
XX
RN   [2]
RP   1-9344
RC   pCS19 from pBR322 & URA3 gene & trp terminator & CEN15 & cI gene/pR
RA   Sengstag C.G.;
RT   "The sequence of Saccharomyces cerevisiae cloning vector pCS19
RT   allowing direct selection for DNA inserts";
RL   Gene 124:141-142(1993).
XX
CC   Shuttle vector for construction of genomic libraries that permits
CC   direct selection of recombinants. Insertion into the BclI site
CC   inactivates the cI repressor, allowing expression of tetracycline
CC   resistance in recombinants.
CC   Propagation in a dam- host is necessary for cleavage at the BclI
CC   site.
CC   Vector contains the following restriction sites (approximate kb
CC   from nt 1):  BamHI--5.2, 6.4; BglII--0.6; EcoRI--0.0, 4.46;
CC   HindIII--0.84, 2.0, 4.21, 4.33; PstI--0.63, 1.8, 8.59;
CC   SalI--5.48; SmaI--0.9, 4.16; XbaI--2.43; XhoI--3.75.
CC   The order of the major features in the plasmid is: EcoRI - ARS1 -
CC   SmaI - URA3 - XbaI - CEN15 - XhoI - BclI/cI - lambda PR - tetR -
CC   trp terminator - BamHI - pMB1 ori - ampR.  [2]
CC   Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: C.G.Sengstag
CC   NM (pCS19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)(shuttle)(positive selection for inserts)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YRp7 5738bp
FT                   2. yeast, PHO5
FT                   -> YRp7-PHO5
FT                   1. YRp7-PHO5 HindIII 5127..5127
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> plasmid
FT                   1. plasmid XbaI-BamHI, YRp7 306..-..5556
FT                   2. yeast XbaI-BglII 1000bp, CEN15
FT                   -> plasmid2
FT                   1. plasmid2 ClaI, CEN15
FT                   2. XhoI linker 6bp ctcgag
FT                   -> pCS14
FT                   1. pUC18 remove PstI-BamHI 14bp 416..430, MCS/2672bp
FT                   2. pUN121 PstI-BamHI, lambda cI gene/pR
FT                   -> plasmid3
FT                   1. plasmid3 PstI 416..416
FT                   XhoI linker 6bp ctcgag
FT                   -> plasmid4
FT                   1. plasmid4 remove small PvuI-XhoI
FT                   2. pCS14 PvuI-XhoI, CEN15
FT                   -> plasmid5
FT                   1. plasmid5 remove small PvuI-BamHI
FT                   2. pUN121 PvuI-BamHI, ori
FT                   -> plasmid6
FT                   1. pBR322 AvaI 4361bp 1426..1426, 3' to tet gene
FT                   2. E. coli HincII-SmaI 158bp, trp terminator
FT                   -> plasmid7 4519bp
FT                   1. plasmid6 remove small PvuI-SalI, 6099bp
FT                   3. plasmid7 PvuI-SalI 3245bp 652..-..3739, trp term
FT                   -> pCS19 9344bp"
FT   rep_origin      591..601
FT                   /note="ORI ARS"
FT   misc_feature    982..1782
FT                   /note="ANT URA3 gene"
FT   repeat_region   3216..3465
FT                   /standard_name="CEN15 centromere;
FT                   rpt_type=other"
FT   misc_feature    3980..4690
FT                   /gene="GEN cI gene
FT                   tetracycline repressor"
FT   CDS             4914..6104
FT                   /note="ANT E. coli tetracyline resistance (tet)"
FT   attenuator      6262..6401
FT                   /gene="trpA"
FT   misc_feature    8281..9138
FT                   /note="ANT E. coli ampicillin resistance (amp)"
FT   misc_binding    0..0
FT                   /note="SIT BclI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 9344 BP; 2510 A; 2234 C; 2042 G; 2558 T; 0 other;
     aattccacat gttaaaatag tgaaggagca tgttcggcac acagtggacc gaacgtgggg
     taagtgcact agggtccggt taaacggatc tcgcattgat gaggcaacgc taattatcaa
     catatagatt gttatctatc tgcatgaaca cgaaatcttt acttgacgac ttgaggctga
     tggtgtttat gcaaagaaac cactgtgttt aatatgtgtc actgtttgat attactgtca
     gcgtagaaga taatagtaaa agcggttaat aagtgtattt gagataagtg tgataaagtt
     tttacagcga aaagacgata aatacaagaa aatgattacg aggatacgga gagaggtatg
     tacatgtgta tttatatact aagctgccgg cggttgtttg caagaccgag aaaaggctag
     caagaatcgg gtcattgtag cgtatgcgcc tgtgaacatt ctcttcaaca agtttgattc
     cattgcggtg aaatggtaaa agtcaacccc ctgcgatgta tattttcctg tacaatcaat
     caaaaagcca aatgatttag cattatcttt acatcttgtt attttacaga ttttatgttt
     agatctttta tgcttgcttt tcaaaaggcc tgcaggcaag tgcacaaaca atacttaaat
     aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
     gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
     aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
     ctttttcttt ccaatttttt ttttttcgtc attatagaaa tcattacgac cgagattccc
     gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
     gctcttcgca atgtcaacag tacccttagt atattctcca gtagctaggg agcccttgca
     tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ccgccgcctg
     cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
     ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
     aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
     aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
     tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
     agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
     aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
     gcaggttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
     accacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
     ctcggagatt accgaatcaa aaaaatttca aagaaaccgg aatcaaaaaa aagaacaaaa
     aaaaaaaaga tgaattgaaa agctttcggg gctctcttgc cttccaaccc agtcagaaat
     cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa
     cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag
     tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg
     cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg
     attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac
     aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca
     tataataccc agcaagtcag catcggaatc tagataaata ctatcgaaga atagttcagc
     tggtgggttc ttttaacgct tcacctgata agagtagaaa ggttgattat gttctcccgc
     caaatattga tatagtgagt gaaagtggct cccaaactac tcaactaaag gggtcgtcat
     cacccaattc tcactcttca caaaagcgat attctgaggc aaaggacgca catatctata
     acaagcgatc aaaggcagat taatgatttt tcgtggggta ttatgaaagc aatcatgatt
     gagcaaaact ttccatttgt acatatatat atatatatat atatatatct gtgtgtgtat
     aataagcaat tggccatcat ttggctaaaa agttcaagat ttcaggtagt atataagaga
     ataacttccc tcttttgcta aagaaggata tgttctattt cagccttttt gcatcataag
     agcataagcg acagaaaaca cttcgagatg agcttcgaga agttcatttc aattaacttg
     ataaatacat gtagattcat atcatcactg ttcttttcag atactagttt caaaacttcc
     ttgacagaac catttcatgt tgaataatga aaattacttt cacatgtttt agtggaaaaa
     tactgaacaa tgaaatttca atactgcata taataatttt tgttatcaaa tatatatttt
     tcggaagtca tatacattaa ataattaaaa cattattttt aaaaattaaa aaaatatatt
     ttagcaaata gtttttactt ttttttaaat gcaaaataag ttcacgtgat attattaata
     atattttacc caaaaatatg agggcatcgg aaaatttttc agtaatgaat attaatgttc
     atctgaagat agacgaaata ggaacaacaa acagcttata agcacccaat aagtgcgtta
     tgacttctga aaatccggat gtacttttat ctagggtgat aaatgtggtg agagcagcat
     catcgttagc cagtcaagat gtagactttt acaagaattt agacagggga tttagtaaag
     atccgaaagt tgcattcaac aagaatgcgc aaatatgtca acgtatttgg aagtcatctt
     atgtgcgctg ctttaatgtt ttctcatgta agcggacgtc gtctataaac ttcaaacgaa
     ggtaaaaggt tcatagcgct ttttctttgt ctgcacaaag aaatatatat taaattagca
     cgttttcgca tagaacgcaa ctgcacaatg ccaaaaaagg taaaagtgat taaaagagtt
     aattgaatag gcaatctcta aacgaatcgc ctcgaggtga tgattatcag ccagcagaga
     attaaggaaa acagacaggt ttattgagcg cttatctttc cctttatttt tgctgcggta
     agtcgcataa aaaccattct tcataattca atccatttac tatgttatgt tctgagggga
     gtgaaaattc ccctaattcg atgaagattc ttgctcaatt gttatcagct atgcgccgac
     cagaacacct tgccgatcag ccaaacgtct cttcaggcca ctgactagcg ataactttcc
     ccacaacgga acaactctca ttgcatggga tcattgggta ctgtgggttt agtggttgta
     aaaacacctg accgctatcc ctgatcagtt tcttgaaggt aaactcatca cccccaagtc
     tggctatgca gaaatccccg ggctcaacag cctgctcagg gtcaacgaga attaacattc
     cgtcaggaaa gcttggcttg gagcctgttg gtgcggtcat ggaattacct tcaacctcaa
     gccagaatgc agaatcactg gcttttttgg ttgtgcttac ccatctctcc gcatcacctt
     tggtaaaggt tctaagctta ggtgagaaca tccctgcctg aacatgagaa aaaacagggt
     actcatactc acttctaagt gacggctgca tactaaccgc ttcatacatc tcgtagattt
     ctctggcgat tgaagggctg aattcttcaa cgctaacttt gagaattttt gtaagcaatg
     cggcgttata agcatttaat gcattgatgc cattaaataa agcaccaacg cctgactgcc
     ccatccccat cttgtctgcg acagattcct gggataagcc aagttcattt ttcttttttt
     cataaattgc tttaaggcga cgtgcgtcct caagctgctc ttgtgttaat ggtttctttt
     ttgtgctcat acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg
     actattttac ctctggcggt gataatggtt gcatgtacta aggaggttgt atggaacaac
     gcataaccct gaaagattat gcaatgcgct ttgggcaaac caagacagct aaagatcagc
     tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat
     ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct
     tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca
     gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc
     tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag
     ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac
     gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
     cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
     ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
     cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
     gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
     cacgcggcgc atctcggaca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa
     tcagataccc agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa
     tgcaaacaca aaaaccgact ctcgaactgc taacctgcga agggatcccc tcgggcagcg
     ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
     tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
     attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
     cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     aacctataaa aataggcgta tcacgaggcc ctttcgtctt caag
//