Return to this vector's summary.
ID PCS19 preliminary; circular DNA; SYN; 9344 BP.
XX
AC M98046; ATCC77409;
XX
DT 01-DEC-1994 (Rel. 10, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli plasmid vector pCS19 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pCS19 from pBR322 & URA3 gene & trp terminator & CEN15 & cI gene/pR
RA Sengstag C.G., Hinnen A.;
RT "The sequence of the Saccharomyces cerevisiae gene PHO2 codes for
RT a regulatory protein with unusual aminoacid composition";
RL Nucleic Acids Res. 15:233-246(1987).
XX
RN [2]
RP 1-9344
RC pCS19 from pBR322 & URA3 gene & trp terminator & CEN15 & cI gene/pR
RA Sengstag C.G.;
RT "The sequence of Saccharomyces cerevisiae cloning vector pCS19
RT allowing direct selection for DNA inserts";
RL Gene 124:141-142(1993).
XX
CC Shuttle vector for construction of genomic libraries that permits
CC direct selection of recombinants. Insertion into the BclI site
CC inactivates the cI repressor, allowing expression of tetracycline
CC resistance in recombinants.
CC Propagation in a dam- host is necessary for cleavage at the BclI
CC site.
CC Vector contains the following restriction sites (approximate kb
CC from nt 1): BamHI--5.2, 6.4; BglII--0.6; EcoRI--0.0, 4.46;
CC HindIII--0.84, 2.0, 4.21, 4.33; PstI--0.63, 1.8, 8.59;
CC SalI--5.48; SmaI--0.9, 4.16; XbaI--2.43; XhoI--3.75.
CC The order of the major features in the plasmid is: EcoRI - ARS1 -
CC SmaI - URA3 - XbaI - CEN15 - XhoI - BclI/cI - lambda PR - tetR -
CC trp terminator - BamHI - pMB1 ori - ampR. [2]
CC Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C
CC Deposited by: C.G.Sengstag
CC NM (pCS19)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli)(Saccharomyces)
CC CP ()
CC FN (cloning)(shuttle)(positive selection for inserts)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. YRp7 5738bp
FT 2. yeast, PHO5
FT -> YRp7-PHO5
FT 1. YRp7-PHO5 HindIII 5127..5127
FT 2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT -> plasmid
FT 1. plasmid XbaI-BamHI, YRp7 306..-..5556
FT 2. yeast XbaI-BglII 1000bp, CEN15
FT -> plasmid2
FT 1. plasmid2 ClaI, CEN15
FT 2. XhoI linker 6bp ctcgag
FT -> pCS14
FT 1. pUC18 remove PstI-BamHI 14bp 416..430, MCS/2672bp
FT 2. pUN121 PstI-BamHI, lambda cI gene/pR
FT -> plasmid3
FT 1. plasmid3 PstI 416..416
FT XhoI linker 6bp ctcgag
FT -> plasmid4
FT 1. plasmid4 remove small PvuI-XhoI
FT 2. pCS14 PvuI-XhoI, CEN15
FT -> plasmid5
FT 1. plasmid5 remove small PvuI-BamHI
FT 2. pUN121 PvuI-BamHI, ori
FT -> plasmid6
FT 1. pBR322 AvaI 4361bp 1426..1426, 3' to tet gene
FT 2. E. coli HincII-SmaI 158bp, trp terminator
FT -> plasmid7 4519bp
FT 1. plasmid6 remove small PvuI-SalI, 6099bp
FT 3. plasmid7 PvuI-SalI 3245bp 652..-..3739, trp term
FT -> pCS19 9344bp"
FT rep_origin 591..601
FT /note="ORI ARS"
FT misc_feature 982..1782
FT /note="ANT URA3 gene"
FT repeat_region 3216..3465
FT /standard_name="CEN15 centromere;
FT rpt_type=other"
FT misc_feature 3980..4690
FT /gene="GEN cI gene
FT tetracycline repressor"
FT CDS 4914..6104
FT /note="ANT E. coli tetracyline resistance (tet)"
FT attenuator 6262..6401
FT /gene="trpA"
FT misc_feature 8281..9138
FT /note="ANT E. coli ampicillin resistance (amp)"
FT misc_binding 0..0
FT /note="SIT BclI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 9344 BP; 2510 A; 2234 C; 2042 G; 2558 T; 0 other;
aattccacat gttaaaatag tgaaggagca tgttcggcac acagtggacc gaacgtgggg
taagtgcact agggtccggt taaacggatc tcgcattgat gaggcaacgc taattatcaa
catatagatt gttatctatc tgcatgaaca cgaaatcttt acttgacgac ttgaggctga
tggtgtttat gcaaagaaac cactgtgttt aatatgtgtc actgtttgat attactgtca
gcgtagaaga taatagtaaa agcggttaat aagtgtattt gagataagtg tgataaagtt
tttacagcga aaagacgata aatacaagaa aatgattacg aggatacgga gagaggtatg
tacatgtgta tttatatact aagctgccgg cggttgtttg caagaccgag aaaaggctag
caagaatcgg gtcattgtag cgtatgcgcc tgtgaacatt ctcttcaaca agtttgattc
cattgcggtg aaatggtaaa agtcaacccc ctgcgatgta tattttcctg tacaatcaat
caaaaagcca aatgatttag cattatcttt acatcttgtt attttacaga ttttatgttt
agatctttta tgcttgcttt tcaaaaggcc tgcaggcaag tgcacaaaca atacttaaat
aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
ctttttcttt ccaatttttt ttttttcgtc attatagaaa tcattacgac cgagattccc
gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
gctcttcgca atgtcaacag tacccttagt atattctcca gtagctaggg agcccttgca
tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ccgccgcctg
cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
gcaggttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
accacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
ctcggagatt accgaatcaa aaaaatttca aagaaaccgg aatcaaaaaa aagaacaaaa
aaaaaaaaga tgaattgaaa agctttcggg gctctcttgc cttccaaccc agtcagaaat
cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa
cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag
tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg
cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg
attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac
aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca
tataataccc agcaagtcag catcggaatc tagataaata ctatcgaaga atagttcagc
tggtgggttc ttttaacgct tcacctgata agagtagaaa ggttgattat gttctcccgc
caaatattga tatagtgagt gaaagtggct cccaaactac tcaactaaag gggtcgtcat
cacccaattc tcactcttca caaaagcgat attctgaggc aaaggacgca catatctata
acaagcgatc aaaggcagat taatgatttt tcgtggggta ttatgaaagc aatcatgatt
gagcaaaact ttccatttgt acatatatat atatatatat atatatatct gtgtgtgtat
aataagcaat tggccatcat ttggctaaaa agttcaagat ttcaggtagt atataagaga
ataacttccc tcttttgcta aagaaggata tgttctattt cagccttttt gcatcataag
agcataagcg acagaaaaca cttcgagatg agcttcgaga agttcatttc aattaacttg
ataaatacat gtagattcat atcatcactg ttcttttcag atactagttt caaaacttcc
ttgacagaac catttcatgt tgaataatga aaattacttt cacatgtttt agtggaaaaa
tactgaacaa tgaaatttca atactgcata taataatttt tgttatcaaa tatatatttt
tcggaagtca tatacattaa ataattaaaa cattattttt aaaaattaaa aaaatatatt
ttagcaaata gtttttactt ttttttaaat gcaaaataag ttcacgtgat attattaata
atattttacc caaaaatatg agggcatcgg aaaatttttc agtaatgaat attaatgttc
atctgaagat agacgaaata ggaacaacaa acagcttata agcacccaat aagtgcgtta
tgacttctga aaatccggat gtacttttat ctagggtgat aaatgtggtg agagcagcat
catcgttagc cagtcaagat gtagactttt acaagaattt agacagggga tttagtaaag
atccgaaagt tgcattcaac aagaatgcgc aaatatgtca acgtatttgg aagtcatctt
atgtgcgctg ctttaatgtt ttctcatgta agcggacgtc gtctataaac ttcaaacgaa
ggtaaaaggt tcatagcgct ttttctttgt ctgcacaaag aaatatatat taaattagca
cgttttcgca tagaacgcaa ctgcacaatg ccaaaaaagg taaaagtgat taaaagagtt
aattgaatag gcaatctcta aacgaatcgc ctcgaggtga tgattatcag ccagcagaga
attaaggaaa acagacaggt ttattgagcg cttatctttc cctttatttt tgctgcggta
agtcgcataa aaaccattct tcataattca atccatttac tatgttatgt tctgagggga
gtgaaaattc ccctaattcg atgaagattc ttgctcaatt gttatcagct atgcgccgac
cagaacacct tgccgatcag ccaaacgtct cttcaggcca ctgactagcg ataactttcc
ccacaacgga acaactctca ttgcatggga tcattgggta ctgtgggttt agtggttgta
aaaacacctg accgctatcc ctgatcagtt tcttgaaggt aaactcatca cccccaagtc
tggctatgca gaaatccccg ggctcaacag cctgctcagg gtcaacgaga attaacattc
cgtcaggaaa gcttggcttg gagcctgttg gtgcggtcat ggaattacct tcaacctcaa
gccagaatgc agaatcactg gcttttttgg ttgtgcttac ccatctctcc gcatcacctt
tggtaaaggt tctaagctta ggtgagaaca tccctgcctg aacatgagaa aaaacagggt
actcatactc acttctaagt gacggctgca tactaaccgc ttcatacatc tcgtagattt
ctctggcgat tgaagggctg aattcttcaa cgctaacttt gagaattttt gtaagcaatg
cggcgttata agcatttaat gcattgatgc cattaaataa agcaccaacg cctgactgcc
ccatccccat cttgtctgcg acagattcct gggataagcc aagttcattt ttcttttttt
cataaattgc tttaaggcga cgtgcgtcct caagctgctc ttgtgttaat ggtttctttt
ttgtgctcat acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg
actattttac ctctggcggt gataatggtt gcatgtacta aggaggttgt atggaacaac
gcataaccct gaaagattat gcaatgcgct ttgggcaaac caagacagct aaagatcagc
tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat
ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct
tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca
gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc
tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag
ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac
gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
cacgcggcgc atctcggaca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa
tcagataccc agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa
tgcaaacaca aaaaccgact ctcgaactgc taacctgcga agggatcccc tcgggcagcg
ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
aacctataaa aataggcgta tcacgaggcc ctttcgtctt caag
//