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ID   PCS832     preliminary; circular DNA; SYN; 7458 BP.
XX
AC   IG9969;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCS832 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pTB2 from pSC101 & B.licheniformis, amp gene
RC   [pOPI from Bacillus]
RC   [pOPIdelta6 from pOPI]
RC   pOG2110 from pTB2 & pOPIdelta6
RC   pCS832 from pC194 & pUB110
RC   pCS1006 from pCS832
RC   pOG1196 from pCS1006
RC   pOG2165 from pOG1196 & pOG2110
RC   [pOG2326 from pOG1196]
RA   Gray O., Chang S.;
RT   "Molecular cloning and expression of Bacillus licheniformis
RT   beta-lactamase gene in Eschercihia coli and Bacillus subtilis";
RL   J. Bacteriol. 145:422-428(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pCS832)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pC194)(pUB110)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pC194 MboI 2910bp 1..1, or 2005..2005, cat
FT                   2. pUB110 BamHI 4548bp 789..789, kan gene
FT                   -> pCS832 7458bp"
FT   -               1..788
FT                   /note="pUB110 1..788 788bp
FT                   BamHI =        G^GATCC
FT                   MboI = Sau3AI = ^GATC"
FT   -               789..3698
FT                   /note="pC194 1..2910 2910bp
FT                   MboI = Sau3AI = ^GATC
FT                   BamHI =        G^GATCC"
FT   -               3699..7458
FT                   /note="pUB110 789..4548 3760bp"
FT   rep_origin      0..0
FT                   /note="ORI pUB110"
XX
SQ   Sequence 7458 BP; 2278 A; 1486 C; 993 G; 2701 T; 0 other;
     gaattcctta gtgctttcat agattaaact cacatcacgc tttaaatcgc ttattttaga
     ctttaaagac ttgttttctt caagcaactc attataatca tttacatttt cattaaatcg
     ctctacaaga ccactatatt tttctttaac ttgcccatgt tctttactta attttttata
     ttctctcgcc atatcagtac tcatgagatt tctaacatgc tgttttaacc tatcgttatc
     tctcgcagca gtcactaagt ttttataatc acgctccgat ataacaacat ttttggttgg
     tttcttttct gttttcatta tttcttttcc caaaccaaac atggactttt cacccgttgg
     cacttcaaca cttttcatgt gtcgtttcgc tggtacttct aaatctgatt taactttatc
     gctataagca gtccattcat cttttttaac tgctaaattt ttttctagaa aatcaatctc
     tttttccaaa gtttgttttt taaatttagc tgtctcaata tgtttacggt cagagccacg
     ttcaccacgc ttcaactcaa aaccctgttt tttcatatgc tcggggaatt tatcttgtag
     ccataacagt tcttgacgat taaacacatt ttttccttgc agttttccat cacgcatagg
     cacaacacct aaatgcatgt gaggggtttg ctcatcatta tgaactgttg cataagcaat
     attttgcttg ccatatcgtt cggaaaataa tttataactt tcctcaaaaa atcgtttttg
     ttctcctgga tcaatttctt ttaagtaatc taaatcccca ttttttaatt tctttttagc
     ctctttaaat aatcctgaat aaactaatac ctgtttacct ttaagtgatt tataaaatgc
     atcaaagact ttttgattta tttattaaat aatcactatc tttaccagaa tacttagcca
     tttcatataa ttctttatta ttattttgtc ttattttttg aacttgaact tgtgttattt
     ctgaaatgcc cgttacatca cgccataaat ctaaccattc ttgttggcta atataatatc
     ttttatctgt gaaatacgat ttatttactg caattaacac atgaaaatga ggattataat
     catctctttt tttattatat gtaatctcta acttacgaac atatcccttt ataacactac
     ctactttttt tctctttata agttttctaa aagaattatt ataacgtttt atttcatttt
     ctaattcatc actcattaca ttaggtgtag tcaaagttaa aaagataaac tcctttttct
     cttgctgctt aatatattgc atcatcaaag ataaacccaa tgcatctttt ctagcttttc
     tccaagcaca gacaggacaa aatcgatttt tacaagaatt agctttatat aatttctgtt
     tttctaaagt tttatcagct acaaaagaca gaaatgtatt gcaatcttca actaaatcca
     tttgattctc tccaatatga cgtttaataa atttctgaaa tacttgattt ctttgttttt
     tctcagtata cttttccatg ttataacaca taaaaacaac ttagttttca caaactatga
     caataaaaaa agttgctttt tcccctttct atgtatgttt tttactagtc atttaaaacg
     atacattaat aggtacgaaa aagcaacttt ttttgcgctt aaaaccagtc ataccaataa
     cttaagggta actagcctcg ccggcaatag ttacccttat tatcaagata agaaagaaaa
     ggatttttcg ctacgctcaa atcctttaaa aaaacacaaa agaccacatt ttttaatgtg
     gtctttattc ttcaactaaa gcacccatta gttcaacaaa cgaaaattgg ataaagtggg
     atatttttaa aatatatatt tatgttacag taatattgac ttttaaaaaa ggattgattc
     taatgaagaa agcagacaag taagcctcct aaattcactt tagataaaaa tttaggaggc
     atatcaaatg aactttaata aaattgattt agacaattgg aagagaaaag agatatttaa
     tcattatttg aaccaacaaa cgacttttag tataaccaca gaaattgata ttagtgtttt
     ataccgaaac ataaaacaag aaggatataa attttaccct gcatttattt tcttagtgac
     aagggtgata aactcaaata cagcttttag aactggttac aatagcgacg gagagttagg
     ttattgggat aagttagagc cactttatac aatttttgat ggtgtatcta aaacattctc
     tggtatttgg actcctgtaa agaatgactt caaagagttt tatgatttat acctttctga
     tgtagagaaa tataatggtt cggggaaatt gtttcccaaa acacctatac ctgaaaatgc
     tttttctctt tctattattc catggacttc atttactggg tttaacttaa atatcaataa
     taatagtaat taccttctac ccattattac agcaggaaaa ttcattaata aaggtaattc
     aatatattta ccgctatctt tacaggtaca tcattctgtt tgtgatggtt atcatgcagg
     attgtttatg aactctattc aggaattgtc agataggcct aatgactggc ttttataata
     tgagataatg ccgactgtac tttttacagt cggttttcta atgtcactaa cctgccccgt
     tagttgaaga aggtttttat attacagctc cagatccata tccttctttt tctgaaccga
     cttctccttt ttcgcttctt tattccaatt gctttattga cgttgagcct cggaaccctt
     aacaatccca aaacttgtcg aatggtcggc ttaatagctc acgctatgcc gacattcgtc
     tgcaagttta gttaagggtt cttctcaacg cacaataaat tttctcggca taaatgcgtg
     gtctaatttt tatttttaat aaccttgata gcaaaaaatg ccattccaat acaaaaccac
     atacctataa tcgataacca cataacagtc ataaaaccac tcctttttaa caaactttat
     cacaagaaat atttaaattt taaatgcctt tattttgaat tttaaggggc attttaaaga
     tttaggggta aatcatatag ttttatgcct aaaaacctac agaagctttt aaaaagcaaa
     tatgagccaa ataaatatat tctaattcta caaacaaaaa tttgagcaaa ttcagtgtcg
     attttttaag acactgccca gttacatgca aattaaaatt ttcatgattt tttatagttc
     ctaacagggt taaaatttgt ataacgaaag tataatgttt atataacgtt agtataataa
     agcattttaa cattatactt ttgataatcg tttatcgtcg tcatcacaat aacttttaaa
     atactcgtgc ataattcacg ctgacctccc aataactaca tggtgttatc gggaggtcag
     ctgttagcac ttatattttg ttattgttct tcctcgattt cgtctatcat tttgtgatta
     atttctcttt tttcttgttc tgttaagtca taaagttcac tagctaaata ctctttttgt
     ttccaaatat aaaaaatttg atagatatat tacggttgga tccagttgct caaaaaaatc
     tcggtcagat gttactagca actcatttac aagaacagca tctttcctcc gtttttcttg
     tacctgtttt ttgtgattca ataatttctt tgacacgttc gttgtaatca atatttttat
     catttttcaa atcataattt tcacgtgttc gctcatggtc aatatcatca ttcgttctac
     tttttcgctc tctttgatta tgaaattgca tgccttttag tccagctgat ttcacttttt
     gcattctaca aactgcataa ctcatatgta aatcgctcct ttttaggtgg cacaaatgtg
     aggcattttc gctctttccg gcaaccactt ccaagtaaag tataacacac tatactttat
     attcataaag tgtgtgctct gcgaggctgt cggcagtgcc gaccaaaacc ataaaacctt
     taagaccttt ctttttttta cgagaaaaaa gaaacaaaaa aacctgccct ctgccacctc
     agcaaagggg ggttttgctc tcgtgctcgt ttaaaaatca gcaagggaca ggtagtattt
     tttgagaaga tcactcaaaa aatctccacc tttaaaccct tgccaatttt tattttgtcc
     gttttgtcta gcttaccgaa agccagactc agcaagaata aaatttttat tgtctttcgg
     ttttctagtg taacggacaa aaccactcaa aataaaaaag atacaagaga ggtctctcgt
     atcttttatt cagcaatcgc gcccgattgc tgaacagatt aataatagat tttagctttt
     tatttgttga aaaaagctaa tcaaattgtt gtcgggatca attactgcaa agtctcgttc
     atcccaccac tgatctttta atgatgtatt ggggtgcaaa atgcccaaag gcttaatatg
     ttgatataat tcatcaattc cctctacttc aatgcggcaa ctagcagtac cagcaataaa
     cgactccgca cctgtacaaa ccggtgaatc attactacga gagcgccagc ttcatcactt
     gcctcccata gatgaatccg aacctcatta cacattagaa ctgcgaatcc atcttcatgg
     tgaaccaaag tgaaacctag tttatcgcaa taaaaaccta tactcttttt aatatccccg
     actggcaatg ccgggataga ctgtaacatt ctcacgcata aaatcccctt tcattttcta
     atgtaaatct attaccttat tattaattca attcgctcat aattaatcct ttttcttatt
     acgcaaaatg gcccgattta agcacaccct ttattccgtt aatgcgccat gacagccatg
     ataattacta atactaggag aagttaataa atacgtaacc aacatgatta acaattatta
     gaggtcatcg ttcaaaatgg tatgcgtttt gacacatcca ctatatatcc gtgtcgttct
     gtccactcct gaatcccatt ccagaaattc tctagcgatt ccagaagttt ctcagagtcg
     gaaagttgac cagacattac gaactggcac agatggtcat aacctgaagg aagatctgat
     tgcttaactg cttcagttaa gaccgaagcg ctcgtcgtat aacagatgcg atgatgcaga
     ccaatcaaca tggcacctgc cattgctacc tgtacagtca aggatggtag aaatgttgtc
     ggtccttgca cacgaatatt acgccatttg cctgcatatt caaacagctc ttctacgata
     agggcacaaa tcgcatcgtg gaacgtttgg gcttctaccg atttagcagt ttgatacact
     ttctctaagt atccacctga atcataaatc ggcaaaatag agaaaaattg accatgtgta
     agcggccaat ctgattccac ctgagatgca taatctagta gaatctcttc gctatcaaaa
     ttcacttcca ccttccactc accggttgtc cattcatggc tgaactctgc ttcctctgtt
     gacatgacac acatcatctc aatatccgaa tagggcccat cagtctgacg accaagagag
     ccataaacac caatagcctt aacatcatcc ccatatttat ccaatattcg ttccttaatt
     tcatgaacaa tcttcattct ttcttctcta gtcattatta ttggtccatt cactattctc
     attccctttt cagataattt tagatttgct tttctaaata agaatatttg gagagcaccg
     ttcttattca gctattaata actcgtcttc ctaagcatcc ttcaatcctt ttaataacaa
     ttatagcatc taatcttcaa caaactggcc cgtttgttga actactcttt aataaaataa
     tttttccgtt cccaattcca cattgcaata atagaaaatc catcttcatc ggctttttcg
     tcatcatctg tatgaatcaa atcgccttct tctgtgtcat caaggtttaa ttttttatgt
     atttctttta acaaaccacc ataggagatt aaccttttac ggtgtaaacc ttcctccaaa
     tcagacaaac gtttcaaatt cttttcttca tcatcggtca taaaatccgt atcctttaca
     ggatattttg cagtttcgtc aattgccgat tgtatatccg atttatattt atttttcggt
     cgaatcattt gaacttttac atttggatca tagtctaatt tcattgcctt tttccaaaat
     tgaatccatt gtttttgatt cacgtagttt tctgtattct taaaataagt tggttccaca
     cataccaata catgcatgtg ctgattataa gaattatctt tattatttat tgtcacttcc
     gttgcacgca taaaaccaac aagattttta ttaatttttt tatattgcat cattcggcga
     aatccttgag ccatatctga caaactctta tttaattctt cgccatcata aacattttta
     actgttaatg tgagaaacaa ccaacgaact gttggctttt gtttaataac ttcagcaaca
     accttttgtg actgaatgcc atgtttcatt gctctcctcc agttgcacat tggacaaagc
     ctggatttac aaaaccacac tcgatacaac tttctttcgc ctgtttcacg attttgttta
     tactctaata tttcagcaca atcttttact ctttcagcct ttttaaattc aagaatatgc
     agaagttcaa agtaatcaac attagcgatt ttcttttctc tccatggtct cacttttcca
     ctttttgtct tgtccactaa aacccttgat ttttcatctg aataaatgct actattagga
     cacataatat taaaagaaac ccccatctat ttagttattt gtttagtcac ttataacttt
     aacagatggg gtttttctgt gcaaccaatt ttaagggttt tcaatacttt aaaacacata
     cataccaaca cttcaacgca cctttcagca actaaaataa aaatgacgtt atttctatat
     gtatcaagat aagaaagaac aagttcaaaa ccatcaaaaa aagacacctt ttcaggtgct
     ttttttattt tataaactca ttccctgatc tcgacttcgt tcttttttta cctctcggtt
     atgagttagt tcaaattcgt tctttttagg ttctaaatcg tgtttttctt ggaattgtgc
     tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg tttttaaagg cttttaagcc
     gtctgtacgt tccttaag
//