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ID   PD4        preliminary; circular DNA; SYN; 5553 BP.
XX
AC   IG0036;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pD4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pD1 from phage f1 & pBR322]
RC   pD4 from phage f1 & pBR322
RC   pD9 from phage f1 & pBR322
RA   Dotto G.P., Horiuchi K.;
RT   "Replication of a plasmid containing two origins of bacteriophage f1";
RL   J. Mol. Biol. 153:169-176(1981).
XX
RN   [2]
RC   R219, R229 from phage f1
RC   R242 from R229 & pGL101
RC   NZ1, NZ2, NZ3 from R242
RC   pBR325::pD1 from pBR325 & pD1
RC   from R219 & pBR325::pD1
RC   pD6:pD7 from pD6 & pD7
RC   from R222 & pD6:pD7
RC   from R222 & pD8
RC   pD3 from phage f1 & pGL101
RC   pBR322:pD3 from pBR322 & pD3
RC   pD6 from phage f1 & pBR322:pD3
RC   pD4 from phage f1 & pBR322
RC   pD7, pD8 from phage f1 & pD6
RC   pGL101:pD4 from pGL101 & pD4
RC   pD5 from phage f1 & pGL101:pD4
RC   from phage f1 & pD5
RA   Dotto G.P., Enea V., Zinder N.D.;
RT   "Functional analysis of bacteriophage f1 intergenic region";
RL   Virology 114:463-473(1981).
XX
RN   [3]
RC   pD2 from phage f1
RA   Dotto G.P., Enea V., Zinder N.D.;
RT   "Gene II of phage f1: its functions and its products";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:5421-5424(1981).
XX
CC   NM (pD4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(sequencing)(mutagenesis)
CC   SE ()
CC   PA (pBR322)(f1)
CC   BR (pD9)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360
FT                   bacterial alkaline phosphatase
FT                   2. phage f1 HgaI-HindII
FT                   Klenow:Klenow
FT                   EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT                   EcoRI-EcoRI
FT                   -> pD4 5553bp"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   rep_origin      2537..2537
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(3295..4155)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      4367..5621
FT                   /note="ORI bacteriophage f1 intergenic region,
FT                   (M13IG), as HindII (base 1) to HgaI (base 5148),
FT                   inserted into the EcoRI site in pBR322"
XX
SQ   Sequence 5553 BP; 1260 A; 1484 C; 1378 G; 1431 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc
     acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
     atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
     cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac
     gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     tcattacccc catgaacaga aattccccct tacacggagg catcaagtga ccaaacagga
     aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     aggcgtatca cgaggccctt tcgtcttcaa gaattcattg agcgtcaaaa tgtaggtatt
     tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga tattaccagc
     aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt
     attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat
     tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc
     ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa
     gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta
     tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata tgattgacat
     gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct caggcaatga
     cctgatagcc tttgtagacc tctcaaaaat agctaccctc tccggcatga atttatcagc
     tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt ctcacccgtt
     tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg gttctaaaaa
     tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg gtcataatgt
     ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt ttgctaattc
     tttgccttgc ctgtatgatt tattggatgt tag
//