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ID PD4 preliminary; circular DNA; SYN; 5553 BP.
XX
AC IG0036;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pD4 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC [pD1 from phage f1 & pBR322]
RC pD4 from phage f1 & pBR322
RC pD9 from phage f1 & pBR322
RA Dotto G.P., Horiuchi K.;
RT "Replication of a plasmid containing two origins of bacteriophage f1";
RL J. Mol. Biol. 153:169-176(1981).
XX
RN [2]
RC R219, R229 from phage f1
RC R242 from R229 & pGL101
RC NZ1, NZ2, NZ3 from R242
RC pBR325::pD1 from pBR325 & pD1
RC from R219 & pBR325::pD1
RC pD6:pD7 from pD6 & pD7
RC from R222 & pD6:pD7
RC from R222 & pD8
RC pD3 from phage f1 & pGL101
RC pBR322:pD3 from pBR322 & pD3
RC pD6 from phage f1 & pBR322:pD3
RC pD4 from phage f1 & pBR322
RC pD7, pD8 from phage f1 & pD6
RC pGL101:pD4 from pGL101 & pD4
RC pD5 from phage f1 & pGL101:pD4
RC from phage f1 & pD5
RA Dotto G.P., Enea V., Zinder N.D.;
RT "Functional analysis of bacteriophage f1 intergenic region";
RL Virology 114:463-473(1981).
XX
RN [3]
RC pD2 from phage f1
RA Dotto G.P., Enea V., Zinder N.D.;
RT "Gene II of phage f1: its functions and its products";
RL Proc. Natl. Acad. Sci. U.S.A. 78:5421-5424(1981).
XX
CC NM (pD4)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)(sequencing)(mutagenesis)
CC SE ()
CC PA (pBR322)(f1)
CC BR (pD9)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 EcoRI 4361bp 4360..4360
FT bacterial alkaline phosphatase
FT 2. phage f1 HgaI-HindII
FT Klenow:Klenow
FT EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT EcoRI-EcoRI
FT -> pD4 5553bp"
FT CDS 86..1276
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT rep_origin 2537..2537
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(3295..4155)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 4367..5621
FT /note="ORI bacteriophage f1 intergenic region,
FT (M13IG), as HindII (base 1) to HgaI (base 5148),
FT inserted into the EcoRI site in pBR322"
XX
SQ Sequence 5553 BP; 1260 A; 1484 C; 1378 G; 1431 T; 0 other;
agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc
acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac
gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
tcattacccc catgaacaga aattccccct tacacggagg catcaagtga ccaaacagga
aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
aggcgtatca cgaggccctt tcgtcttcaa gaattcattg agcgtcaaaa tgtaggtatt
tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga tattaccagc
aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt
attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat
tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc
ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa
gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta
tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata tgattgacat
gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct caggcaatga
cctgatagcc tttgtagacc tctcaaaaat agctaccctc tccggcatga atttatcagc
tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt ctcacccgtt
tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg gttctaaaaa
tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg gtcataatgt
ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt ttgctaattc
tttgccttgc ctgtatgatt tattggatgt tag
//