back Return to this vector's summary.
ID   PDB31      preliminary; circular DNA; SYN; 2898 BP.
XX
AC   U09942;
XX
DT   15-JUN-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Pseudomonas vector pDB31 - complete.
XX
KW   cloning vector; ampicillin resistance; lacZ gene; multicloning site;
KW   phagemid; T7 promoter.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2898
RC   pDB31 from pTZ18R
RA   Boivin R., Bellemare G., Dion P.;
RT   "Novel narrow-host-range vectors for direct cloning of foreign DNA
RT   in Pseudomonas";
RL   Curr. Microbiol. 28:41-47(1994).
XX
RN   [2]
RP   1-2898
RC   pDB31
RA   Boivin R.;
RT   ;
RL   Submitted (22-MAY-1994) by:
RL   Boivin R., Sciences Forestieres, Universite Laval,
RL   Pavillon Charles-Eugene Marchand, Cite Universitaire, Ste-Foy,
RL   Quebec, G1K 7P4, Canada.
XX
CC   NCBI gi: 498575
CC   NM (pDB31)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pTZ18R 2861bp
FT                   linker, MCS
FT                   -> pDB31 2898bp"
FT   misc_feature    165..208
FT                   /note="MCS synthetic"
FT   CDS             complement(1440..2300)
FT                   /gene="bla"
FT                   /note="ANT E.coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             complement(join(2861..2898,1..283))
FT                   /gene="lacZ"
FT                   /note="GEN E.coli beta-galactosidase gene (lacZ)"
XX
SQ   Sequence 2898 BP; 703 A; 742 C; 706 G; 747 T; 0 other;
     cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctagca ctagtgggcc
     cccgcggccg cgtcgacgcg ttaacgatcg actctagagg atccccgggt accgagctcg
     aattccctat agtgagtcgt attaaattcg taatcatggt catagctgtt tcctgtgtga
     aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacaaaa
     tattaacgtt tacaattt
//