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ID   PDBPV11426 preliminary; circular DNA; SYN; 10578 BP.
XX
AC   ATCC37134;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pdBPV-1(142-6) - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pd1BPV69T(51-1) from pML2 & pBPV69T(43-1)
RC   pd1BPV69T(54-2) from pd1BPV69T(51-1)
RC   pML2d from pd1BPV69T(51-1)
RC   pdBPV-1(142-6) from pML2d & pBPV-1(8-2)
RA   Sarver N., Byrne J.C., Howley P.M.;
RT   "Transformation and replication in mouse cells of a bovine
RT   papillomavirus-pML2 plasmid vector that can be rescued in
RT   bacteria";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:7147-7151(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   A shuttle vector which replicates as a stable, nonintegrated,
CC   multicopy plasmid in transformed mouse cells and in E.coli. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pdBPV-1 142-6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   HO (C127I cell line [ATCC CRL1616])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pML2)(BPV-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI-HindIII 4015bp 376..4361..30
FT                   fill in:fill in
FT                   2. BPV-1 left end-ClaI 5475bp 1..5475, BPV-1 69% frag
FT                   \ BPV-1 = 7945bp
FT                   :blunt end
FT                   -> pBPV69T(43-1) 9490bp
FT                   1. pML2 BamHI 2970bp 306..306
FT                   2. pBPV69T(43-1) BamHI-HindIII 4442bp -3..1..4439,
FT                   \ pML2 HindIII 2930/BPV-1 HindIII 4439
FT                   -> pd1BPV69T(51-1) 7412bp
FT                   1. pd1BPV69T(51-1) HindIII 7412bp,
FT                   \ pML2 HindIII 2930
FT                   BamHI linker 6bp ggatcc
FT                   -> pd1BPV69T(54-2) 7422bp
FT                   1. pd1BPV69T(54-2) remove BamHI-BamHI 4788bp, 2634bp
FT                   -> pML2d 2634bp [unique BamHI]
FT                   1. pBR322 BamHI 4361bp 376..376
FT                   fill in:fill in
FT                   2. BPV-1 7945bp 1..7945
FT                   -> pBPV-1(8-2) 12306bp
FT                   1. pML2d BamHI 2630bp 306..2934..1bp
FT                   2. pBPV-1(8-2) BamHI-BamHI 7945bp, BPV-1
FT                   -> pdBPV-1(142-6) 10590bp"
FT   -               1..2628
FT                   /note="pML2 306..2933 2628bp"
FT   -               2629..2629
FT                   /note="g 1bp"
FT   -               2630..2633
FT                   /note="gatc 4bp
FT                   BamHI = G^GATC C
FT                   \              ctaga..."
FT   -               2634..10578
FT                   /note="BPV-1 1..7945 7945bp
FT                   \  atccgg
FT                   BamHI = G^GATCC"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 10578 BP; 2877 A; 2416 C; 2557 G; 2727 T; 1 other;
     gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     tttcgtcttc aagaattctc atgtttgaca gcttatcatc gataagctgg atcgttaaca
     ataatcacac catcaccgtt ttttcaagcg ggaaaaaata gccagctaac tataaaaagc
     tgctgacaga ccccggtttt cacatggacc tgaaaccttt tgcaagaacc aatccattct
     cagggttgga ttgtctgtgg tgcagagagc ctcttacaga agttgatgct tttaggtgca
     tggtcaaaga ctttcatgtt gtaattcggg aaggctgtag atatggtgca tgtaccattt
     gtcttgaaaa ctgtttagct actgaaagaa gactttggca aggtgttcca gtaacaggtg
     aggaagctga attattgcat ggcaaaacac ttgataggct ttgcataaga tgctgctact
     gtgggggcaa actaacaaaa aatgaaaaac atcggcatgt gctttttaat gagcctttct
     gcaaaaccag agctaacata attagaggac gctgctacga ctgctgcaga catggttcaa
     ggtccaaata cccatagaaa cttggatgat tcacctgcag gaccgttgct gattttaagt
     ccatgtgcag gcacacctac caggtctcct gcagcacctg atgcacctga tttcagactt
     ccgtgccatt tcggccgtcc tactaggaag cgaggtccca ctacccctcc gctttcctct
     cccggaaaac tgtgtgcaac agggccacgt cgagtgtatt ctgtgactgt ctgctgtgga
     aactgcggaa aagagctgac ttttgctgtg aagaccagct cgacgtccct gcttggattt
     gaacaccttt taaactcaga tttagacctc ttgtgtccac gttgtgaatc tcgcgagcgt
     catggcaaac gataaaggta gcaattggga ttcgggcttg ggatgctcat atctgctgac
     tgaggcagaa tgtgaaagtg acaaagagaa tgaggaaccc ggggcaggtg tagaactgtc
     tgtggaatct gatcggtatg atagccagga tgaggatttt gttgacaatg catcagtctt
     tcagggaaat cacctggagg tcttccaggc attagagaaa aaggcgggtg aggagcagat
     tttaaatttg aaaagaaaag tattggggag ttcgcaaaac agcagcggtt ccgaagcatc
     tgaaactcca gttaaaagac ggaaatcagg agcaaagcga agattatttg ctgaaaanga
     agctaaccgt gttcttacgc ccctccaggt acagggggag ggggagggga ggcaagaact
     taatgaggag caggcaatta gtcatctaca tctgcagctt gttaaatcta aaaatgctac
     agtttttaag ctggggctct ttaaatcttt gttcctttgt agcttccatg atattacgag
     gttgtttaag aatgataaga ccactaatca gcaatgggtg ctggctgtgt ttggccttgc
     agaggtgttt tttgaggcga gtttcgaact cctaaagaag cagtgtagtt ttctgcagat
     gcaaaaaaga tctcatgaag gaggaacttg tgcagtttac ttaatctgct ttaacacagc
     taaaagcaga gaaacagtcc ggaatctgat ggcaaacacg ctaaatgtaa gagaagagtg
     tttgatgctg cagccagcta aaattcgagg actcagcgca gctctattct ggtttaaaag
     tagtttgtca cccgctacac ttaaacatgg tgctttacct gagtggatac gggcgcaaac
     tactctgaac gagagcttgc agaccgagaa attcgacttc ggaactatgg tgcaatgggc
     ctatgatcac aaatatgctg aggagtctaa aatagcctat gaatatgctt tggctgcagg
     atctgatagc aatgcacggg cttttttagc aactaacagc caagctaagc atgtgaagga
     ctgtgcaact atggtaagac actatctaag agctgaaaca caagcattaa gcatgcctgc
     atatattaaa gctaggtgca agctggcaac tggggaagga agctggaagt ctatcctaac
     tttttttaac tatcagaata ttgaattaat tacctttatt aatgctttaa agctctggct
     aaaaggaatt ccaaaaaaaa actgtttagc atttattggc cctccaaaca caggcaagtc
     tatgctctgc aactcattaa ttcatttttt gggtggtagt gttttatctt ttgccaacca
     taaaagtcac ttttggcttg cttccctagc agatactaga gctgctttag tagatgatgc
     tactcatgct tgctggaggt actttgacac atacctcaga aatgcattgg atggctaccc
     tgtcagtatt gatagaaaac acaaagcagc ggttcaaatt aaagctccac ccctcctggt
     aaccagtaat attgatgtgc aggcagagga cagatatttg tacttgcata gtcgggtgca
     aacctttcgc tttgagcagc catgcacaga tgaatcgggt gagcaacctt ttaatattac
     tgatgcagat tggaaatctt tttttgtaag gttatggggg cgtttagacc tgattgacga
     ggaggaggat agtgaagagg atggagacag catgcgaacg tttacatgta gcgcaagaaa
     cacaaatgca gttgattgag aaaagtagtg ataagttgca agatcatata ctgtactgga
     ctgctgttag aactgagaac acactgcttt atgctgcaag gaaaaaaggg gtgactgtcc
     taggacactg cagagtacca cactctgtag tttgtcaaga gagagccaag caggccattg
     aaatgcagtt gtctttgcag gagttaagca aaactgagtt tggggatgaa ccatggtctt
     tgcttgacac aagctgggac cgatatatgt cagaacctaa acggtgcttt aagaaaggcg
     ccagggtggt agaggtggag tttgatggaa atgcaagcaa tacaaactgg tacactgtct
     acagcaattt gtacatgcgc acagaggacg gctggcagct tgcgaaggct ggggctgacg
     gaactgggct ctactactgc accatggccg gtgctggacg catttactat tctcgctttg
     gtgacgaggc agccagattt agtacaacag ggcattactc tgtaagagat caggacagag
     tgtatgctgg tgtctcatcc acctcttctg attttagaga tcgcccagac ggagtctggg
     tcgcatccga aggacctgaa ggagaccctg caggaaaaga agccgagcca gcccagcctg
     tctcttcttt gctcggctcc cccgcctgcg gtcccatcag agcaggcctc ggttgggtac
     gggacggtcc tcgctcgcac ccctacaatt ttcctgcagg ctcggggggc tctattctcc
     gctcttcctc caccccgtgc agggcacggt accggtggac ttggcatcaa ggcaggaaga
     agaggagcag tcgcccgact ccacagagga agaaccagtg actctcccaa ggcgcaccac
     caatgatgga ttccacctgt taaaggcagg agggtcatgc tttgctctaa tttcaggaac
     tgctaaccag gtaaagtgct atcgctttcg ggtgaaaaag aaccatagac atcgctacga
     gaactgcacc accacctggt tcacagttgc tgacaacggt gctgaaagac aaggacaagc
     acaaatactg atcacctttg gatcgccaag tcaaaggcaa gactttctga aacatgtacc
     actacctcct ggaatgaaca tttccggctt tacagccagc ttggacttct gatcactgcc
     attgcctttt cttcatctga ctggtgtact atgccaaatc tatggtttct attgttcttg
     ggactagttg ctgcaatgca actgctgcta ttactgttct tactcttgtt ttttcttgta
     tactgggatc attttgagtg ctcctgtaca ggtctgccct tttaatgcct ttacatcact
     ggctattggc tgtgttttta ctgttgtgtg gatttgattt gttttatata ctgtatgaag
     ttttttcatt tgtgcttgta ttgctgtttg taagtttttt actagagttt gtattccccc
     tgctcagatt ttatatggtt taagctgcag caataaaaat gagtgcacga aaaagagtaa
     aacgtgccag tgcctatgac ctgtacagga catgcaagca agcgggcaca tgtccaccag
     atgtgatacc aaaggtagaa ggagatacta tagcagataa aattttgaaa tttgggggtc
     ttgcaatcta cttaggaggg ctaggaatag gaacatggtc tactggaagg gttgctgcag
     gtggatcacc aaggtacaca ccactccgaa cagcagggtc cacatcatcg cttgcatcaa
     taggatccag agctgtaaca gcagggaccc gccccagtat aggtgcgggc attcctttag
     acacccttga aactcttggg gccttgcgtc caggggtgta tgaggacact gtgctaccag
     aggcccctgc aatagtcact cctgatgctg ttcctgcaga ttcagggctt gatgccctgt
     ccataggtac agactcgtcc acggagaccc tcattactct gctagagcct gagggtcccg
     aggacatagc ggttcttgag ctgcaacccc tggaccgtcc aacttggcaa gtaagcaatg
     ctgttcatca gtcctctgca taccacgccc ctctgcagct gcaatcgtcc attgcagaaa
     catctggttt agaaaatatt tttgtaggag gctcgggttt aggggataca ggaggagaaa
     acattgaact gacatacttc gggtccccac gaacaagcac gccccgcagt attgcctcta
     aatcacgtgg cattttaaac tggttcagta aacggtacta cacacaggtg cccacggaag
     atcctgaagt gttttcatcc caaacatttg caaacccact gtatgaagca gaaccagctg
     tgcttaaggg acctagtgga cgtgttggac tcagtcaggt ttataaacct gatacactta
     caacacgtag cgggacagag gtgggaccac agctacatgt caggtactca ttgagtacta
     tacatgaaga tgtagaagca atcccctaca cagttgatga aaatacacag ggacttgcat
     tcgtaccctt gcatgaagag caagcaggtt ttgaggagat agaattagat gattttagtg
     agacacatag actgctacct cagaacacct cttctacacc tgttggtagt ggtgtacgaa
     gaagcctcat tccaactcag gaatttagtg caacacggcc tacaggtgtt gtaacctatg
     gctcacctga cacttactct gctagcccag ttactgaccc tgattctacc tctcctagtc
     tagttatcga tgacactact actacaccaa tcattataat tgatgggcac acagttgatt
     tgtacagcag taactacacc ttgcatccct ccttgttgag gaaacgaaaa aaacggaaac
     atgcctaatt ttttttgcag atggcgttgt ggcaacaagg ccagaagctg tatctccctc
     caacccctgt aagcaaggtg ctttgcagtg aaacctatgt gcaaagaaaa agcatttttt
     atcatgcaga aacggagcgc ctgctaacta taggacatcc atattaccca gtgtctatcg
     gggccaaaac tgttcctaag gtctctgcaa atcagtatag ggtatttaaa atacaactac
     ctgatcccaa tcaatttgca ctacctgaca ggactgttca caacccaagt aaagagcggc
     tggtgtgggc agtcataggt gtgcaggtgt ccagagggca gcctcttgga ggtactgtaa
     ctgggcaccc cacttttaat gctttgcttg atgcagaaaa tgtgaataga aaagtcacca
     cccaaacaac agatgacagg aaacaaacag gcctagatgc taagcaacaa cagattctgt
     tgctaggctg tacccctgct gaaggggaat attggacaac agcccgtcca tgtgttactg
     atcgtctaga aaatggcgcc tgccctcctc ttgaattaaa aaacaagcac atagaagatg
     gggatatgat ggaaattggg tttggtgcag ccaacttcaa agaaattaat gcaagtaaat
     cagatctacc tcttgacatt caaaatgaga tctgcttgta cccagactac ctcaaaatgg
     ctgaggacgc tgctggtaat agcatgttct tttttgcaag gaaagaacag gtgtatgtta
     gacacatctg gaccagaggg ggctcggaga aagaagcccc taccacagat ttttatttaa
     agaataataa aggggatgcc acccttaaaa tacccagtgt gcattttggt agtcccagtg
     gctcactagt ctcaactgat aatcaaattt ttaatcggcc ctactggcta ttccgtgccc
     agggcatgaa caatggaatt gcatggaata atttattgtt tttaacagtg ggggacaata
     cacgtggtac taatcttacc ataagtgtag cctcagatgg aaccccacta acagagtatg
     atagctcaaa attcaatgta taccatagac atatggaaga atataagcta gcctttatat
     tagagctatg ctctgtggaa atcacagctc aaactgtgtc acatctgcaa ggacttatgc
     cctctgtgct tgaaaattgg gaaataggtg tgcagcctcc tacctcatcg atattagagg
     acacctatcg ctatatagag tctcctgcaa ctaaatgtgc aagcaatgta attcctgcaa
     aagaagaccc ttatgcaggg tttaagtttt ggaacataga tcttaaagaa aagctttctt
     tggacttaga tcaatttccc ttgggaagaa gatttttagc acagcaaggg gcaggatgtt
     caactgtgag aaaacgaaga attagccaaa aaacttccag taagcctgca aaaaaaaaaa
     aaaaataaaa gctaagtttc tataaatgtt ctgtaaatgt aaaacagaag gtaagtcaac
     tgcacctaat aaaaatcact taatagcaat gtgctgtgtc agttgtttat tggaaccaca
     cccggtacac atcctgtcca gcatttgcag tgcgtgcatt gaattattgt gctggctaga
     cttcatggcg cctggcaccg aatcctgcct tctcagcgaa aatgaataat tgctttgttg
     gcaagaaact aagcatcaat gggacgcgtg caaagcaccg gcggcggtag atgcggggta
     agtactgaat tttaattcga cctatcccgg taaagcgaaa gcgacacgct tttttttcac
     acatagcggg accgaacacg ttataagtat cgattaggtc tatttttgtc tctctgtcgg
     aaccagaact ggtaaaagtt tccattgcgt ctgggcttgt ctatcattgc gtctctatgg
     tttttggagg attagacggg gccaccagta atggtgcata gcggatgtct gtaccgccat
     cggtgcaccg atataggttt ggggctcccc aagggactgc tgggatgaca gcttcatatt
     atattgaatg ggcgcataat cagcttaatt ggtgaggaca agctacaagt tgtaacctga
     tctccacaaa gtacgttgcc ggtcggggtc aaaccgtctt cggtgctcga aaccgcctta
     aactacagac aggtcccagc caagtaggcg gatcaaaacc tcaaaaaggc gggagccaat
     caaaatgcag cattatattt taagctcacc gaaaccggta agtaaagact atgtattttt
     tcccagtgaa taattgtt
//