back Return to this vector's summary.
ID   PDELTABLUE preliminary; circular DNA; SYN; 3507 BP.
XX
AC   M88655; ATCC77334;
XX
DT   20-AUG-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector p-delta-blue - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   bases 1-3507
RC   p-delta-blue from pfXblue
RA   Brandt M.E., Vickery L.E.;
RT   "Expression and characterization of human mitochondrial ferredoxin
RT   reductase in Escherichia coli.";
RL   Arch. Biochem. Biophys. 294:735-740(1992).
XX
CC   The insert to be cloned should have a phosphorylated 5' blunt end with
CC   the first base corresponding to the first base of the first codon.
CC   (personal communication)
CC   Expression vector producing proteins with the one additional
CC   N-terminal methionine.  A modified lacZ' allows blue/white screening
CC   of colonies.[1]
CC   Constructed from pfXblue (ATCC 77333) by deletion of lambda cII and
CC   coagulation factor Xa protease recognition sequences (nt 824-943). [1]
CC   Inserts can be cloned into the vector using a filled-in NcoI site and
CC   a MCS restriction site matching the 3' end of the insert.  The vector
CC   supplies the AUG initiator codon. (personal communication)
CC   The order of the major features of the plasmid is:  pMB1 ori - ampR -
CC   lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ'. [1]
CC   Restriction digests of the plasmid give the following sizes (kb):
CC   BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063,
CC   1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25. (personal
CC   communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 208983
CC   NM (p-delta-blue)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pfXblue)
CC   BR ()
CC   OF (phFR also from pcIIhFR)(phFR18+ from pUC/AR18+)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pfXblue remove NsiI-NcoI 167bp 778..945,
FT                   \ 3460bp
FT                   2. oligo NsiI-NcoI 47bp, 824..825 from AT to CC
FT                   -> p-delta-blue 3507bp [NcoI at init]"
FT   promoter        406..466
FT                   /note="PRO bacteriophage lambda PL"
FT   misc_feature    667..688
FT                   /note="5' primer used for construction"
FT   CDS             826..1326
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ),
FT                   modified"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2630..3490)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="MCS NcoI-StyI-EcoRI-Asp718-KpnI-SmaI-XmaI-
FT                   BamHI-XbaI-AccI-SalI-BspMI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique NcoI-EcoRI-Asp718-KpnI-SmaI-XmaI-
FT                   BamHI-XbaI-AccI-SalI-SphI-HindIII"
XX
SQ   Sequence 3507 BP; 918 A; 887 C; 824 G; 878 T; 0 other;
     attatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
     aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcacttct
     ggctggatga tggggcgatt caggctggta tgagtcagca acaccttctt cacgaggcag
     acctcagcgc cggtgatgcc gacgatgcgt ccggcgtaga ggatctctca cctaccaaac
     aatgcccccc tgcaaaaaat aaattcatat aaaaaacata cagataacca tctgcggtga
     taaattatct ctggcggtgt tgacataaat accactggcg gtgatactga gcacatcagg
     caggacgcac tgaccaccat gaaggtgacg ctcttaaaat taagccctga agaagggcag
     cattcaaagc agaaggcttt ggggtgtgtg atacgaaacg aagcattggc gagctcgccc
     ctaaagatca acaaggccga aagatttttt taactataaa cgctgatgga ggcgtttatg
     cccaagaggt aaagcccttc ccgagtaaca aaaaaacaac agcataaata accccgctct
     tacacattcc agccctgaaa aagggcatca aattaaacca cacctatggt gtatgcattt
     atttgcatac attcaatcaa ttgttatcta aggaaatact tacccatggt tacgaattcg
     agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
     gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
     gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atacggtcgt cgtcccctca
     aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg
     gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
     gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttcctatt
     ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa
     ccgcggtaca tatgatggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     gaatactcat actcttcctt tttcaat
//