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ID   PDEPO      preliminary; circular DNA; SYN; 2677 BP.
XX
AC   IG9914;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pDEPO - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pDEPO from pBR322 & pUC8
RA   Pierson D., Kolter R., Yanofsky C.;
RT   ;
RL   Unpublished (1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pDEPO)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli CY15070)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove FnuDII-FnuDII 1718bp
FT                   \ 288..2006, tet gene/2643bp
FT                   2. pUC8 EcoRI-HindIII 30bp 231..261, MCS
FT                   -> pDEPO 2673bp [amp, no tet]"
FT   -               1..287
FT                   /note="pBR322 1..287 287bp
FT                   FnuDII = CG^CG
FT                   EcoRI =   G^AATTC"
FT   -               288..321
FT                   /note="pUC8 231..264 34bp
FT                   HindIII = A^AGCT T
FT                   FnuDII =      CG^CG"
FT   -               322..2677
FT                   /note="pBR322 2006..4361 2356bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-BamHI-EcoRI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli tac"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2677 BP; 664 A; 686 C; 662 G; 665 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgaat tcccggggat
     ccgtcgacct gcagccaagc tcggatgaac aggcagacat ctgtgaatcg cttcacgacc
     acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     aaataggcgt atcacgaggc cctttcgtct tcaagaa
//